{"id":21255,"date":"2018-12-20T14:45:41","date_gmt":"2018-12-20T11:45:41","guid":{"rendered":"http:\/\/ihcoedu.uobaghdad.edu.iq\/?p=21255"},"modified":"2018-12-24T12:33:09","modified_gmt":"2018-12-24T09:33:09","slug":"%d9%85%d9%86%d8%a7%d9%82%d8%b4%d8%a9-%d8%b1%d8%b3%d8%a7%d9%84%d8%a9-%d8%b7%d8%a7%d9%84%d8%a8%d8%a9-%d8%a7%d9%84%d9%85%d8%a7%d8%ac%d8%b3%d8%aa%d9%8a%d8%b1-%d8%b2%d9%8a%d9%86%d8%a8-%d8%b9%d8%a8","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=21255","title":{"rendered":"\u0645\u0646\u0627\u0642\u0634\u0629 \u0631\u0633\u0627\u0644\u0629 \u0637\u0627\u0644\u0628\u0629 \u0627\u0644\u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u00a0\u0632\u064a\u0646\u0628 \u0639\u0628\u0627\u0633 \u0639\u0628\u062f \u0627\u0644\u062c\u0628\u0627\u0631 \u0627\u0644\u0643\u0628\u064a\u0633\u064a \u0645\u0646 \u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629"},"content":{"rendered":"

<\/h4>\n

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\"\"<\/strong><\/span><\/h4>\n

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\u00a0<\/strong><\/span><\/h4>\n

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\u0628\u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0627\u0644\u0633\u0627\u0644\u0628\u0629.<\/strong><\/span><\/h4>\n

\u0648\u0645\u0646 \u0627\u0644\u0645\u062b\u064a\u0631 \u0644\u0644\u0627\u0647\u062a\u0645\u0627\u0645 \u0623\u0646 \u0646\u062a\u0627\u0626\u062c \u0645\u0633\u062a\u0648\u064a\u0627\u062a <\/strong>IL-6<\/strong> \u0648 <\/strong>IL-1\u03b2<\/strong> \u0648 <\/strong>IL-33<\/strong> \u0623\u0638\u0647\u0631\u062a \u0641\u0631\u0648\u0642\u0627\u064b \u0645\u0639\u0646\u0648\u064a\u0629 (<\/strong>p<0.05<\/strong>) \u0641\u064a \u0627\u0644\u0645\u062c\u0645\u0648\u0639\u0627\u062a \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0629 \u0628\u0627\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646 \u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 \u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0627\u0644\u0645\u0648\u062c\u0628\u0629 \u0628\u0637\u0631\u064a\u0642\u0629 \u062a\u0639\u062a\u0645\u062f \u0639\u0644\u0649 \u0627\u0644\u062c\u0631\u0639\u0629. \u0623\u064a\u0636\u0627 \u0648\u062c\u062f\u062a \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0641\u0631\u0648\u0642\u064b\u0627 \u0645\u0639\u0646\u0648\u064a\u0629 \u0641\u064a \u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0627\u0644\u0645\u0648\u062c\u0628\u0629 \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0627\u0644\u0633\u0627\u0644\u0628\u0629\u060c \u0643\u0630\u0644\u0643 \u0648\u062c\u062f\u062a \u0641\u0631\u0648\u0642 \u0645\u0639\u0646\u0648\u064a\u0629 \u0636\u0645\u0646 \u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0627\u0644\u0645\u0648\u062c\u0628\u0629 \u0627\u0639\u062a\u0645\u0627\u062f\u064b\u0627 \u0639\u0644\u0649 \u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646. \u0628\u0627\u0644\u0625\u0636\u0627\u0641\u0629 \u0625\u0644\u0649 \u0630\u0644\u0643\u060c \u0623\u0638\u0647\u0631\u062a \u0645\u0633\u062a\u0648\u064a\u0627\u062a <\/strong>TNF-\u03b1<\/strong> \u0641\u0631\u0648\u0642\u0627 \u0645\u0639\u0646\u0648\u064a\u0629 (<\/strong>p<0.05<\/strong>) \u0641\u064a \u0627\u0644\u0645\u062c\u0645\u0648\u0639\u0627\u062a \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0629 \u0628\u0640 5 \u0648 10 \u0645\u0644\u063a \u0645\u0646 \u0627\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646 \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0627\u0644\u0645\u0648\u062c\u0628\u0629\u061b \u0648\u0645\u0639 \u0630\u0644\u0643 \u0644\u0645 \u064a\u0644\u0627\u062d\u0638 \u0623\u064a \u0627\u062e\u062a\u0644\u0627\u0641\u0627\u062a \u0645\u0639\u0646\u0648\u064a\u0629 \u0641\u064a \u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0627\u0644\u0645\u0648\u062c\u0628\u0629. \u062a\u0645 \u0641\u062d\u0635 \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0633\u0645\u064a \u0644\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646 \u0639\u0644\u0649 \u0646\u0645\u0648 \u062e\u0637 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 <\/strong>MCF-7<\/strong> \u0628\u0639\u062f \u0627\u0644\u062a\u0639\u0631\u0636 \u0644\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646 \u0644\u0645\u062f\u0629 24 \u0633\u0627\u0639\u0629 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062a\u0631\u0627\u0643\u064a\u0632 12.5 \u0648 25 \u0648 50 \u0648 100 \u0648 200 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644 \u0645\u0646 \u0627\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646. \u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0648\u062c\u0648\u062f \u0641\u0631\u0648\u0642 \u0645\u0639\u0646\u0648\u064a\u0629 \u0641\u064a \u0627\u0644\u062a\u0631\u0643\u064a\u0632 (100 \u060c 200) \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/ \u0644 \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 (<\/strong>p<0.001<\/strong>).<\/strong><\/span><\/h4>\n

\u0643\u0627\u0646\u062a \u0647\u0646\u0627\u0643 \u0623\u064a\u0636\u0627 \u0641\u0631\u0648\u0642 \u0645\u0639\u0646\u0648\u064a\u0629 \u0628\u064a\u0646 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 200 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644 \u0645\u0639 \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0623\u062e\u0631\u0649 (12\u060c 25\u060c 25)\u060c \u0648\u0643\u0630\u0644\u0643 \u0628\u064a\u0646 \u062a\u0631\u0643\u064a\u0632 100 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644 \u0645\u0639 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 25 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644 \u0639\u0646\u062f \u0645\u0633\u062a\u0648\u0649 \u0627\u062d\u062a\u0645\u0627\u0644 <\/strong>p<0.05<\/strong> \u0648\u0639\u0646\u062f <\/strong>p<0.01<\/strong> \u0628\u062a\u0631\u0643\u064a\u0632 12.5 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644. \u0648\u0643\u0634\u0641\u062a \u0646\u062a\u0627\u0626\u062c \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0639\u0646 \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u062a\u062b\u0628\u064a\u0637\u064a \u0644\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646 \u0641\u064a \u0646\u0645\u0648 \u062e\u0637 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 <\/strong>MCF-7<\/strong> \u0648\u0627\u0644\u062a\u064a \u062a\u0645\u062a \u0645\u0639\u0627\u0645\u0644\u062a\u0647\u0627 \u0628\u0640 5 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644 \u0645\u0646 \u0627\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646 \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629. \u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0632\u0627\u0644\u0629 \u0627\u0648 \u0643\u0633\u062d \u0627\u0644\u062c\u0630\u0648\u0631 \u0627\u0644\u062d\u0631\u0629 \u0644\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646\u060c \u0648\u0627\u0644\u0645\u0639\u0631\u0648\u0641 \u0628\u0627\u0633\u0645 \u0627\u062e\u062a\u0628\u0627\u0631 <\/strong>DPPH<\/strong>\u060c \u0648\u062c\u0648\u062f \u0641\u0631\u0642 \u0645\u0639\u0646\u0648\u064a (<\/strong>p<0.05<\/strong>)\u00a0 \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0643\u064a\u0632 25 \u0645\u0644\u063a\u0645.\u0645\u0644-1 \u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 100 \u0645\u0644\u063a\u0645.\u0645\u0644-1. \u0623\u064a\u0636\u0627\u060c \u0623\u0638\u0647\u0631 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0646\u0641\u0633\u0647 \u0641\u0631\u0648\u0642\u0627\u064b \u0645\u0639\u0646\u0648\u064a\u0629 \u0628\u062a\u0631\u0643\u064a\u0632 200 \u0645\u0644\u063a\u0645.\u0645\u0644-1 \u0648\u0644\u0643\u0646 \u0639\u0644\u0649 \u0645\u0633\u062a\u0648\u0649 \u0627\u0644\u0627\u062d\u062a\u0645\u0627\u0644\u064a\u0629 <\/strong>p<0.01<\/strong>\u060c \u0641\u064a \u062d\u064a\u0646 \u0623\u0646 \u062a\u0631\u0643\u064a\u0632 600 \u0645\u0644\u063a\u0645.\u0645\u0644-1 \u0623\u0638\u0647\u0631 \u0641\u0631\u0648\u0642\u0627\u064b \u0645\u0639\u0646\u0648\u064a\u0629 \u0639\u0646\u062f \u0645\u0633\u062a\u0648\u0649 \u0627\u0644\u0627\u062d\u062a\u0645\u0627\u0644\u064a\u0629 <\/strong>p<0.001<\/strong> \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u062a\u0631\u0643\u064a\u0632 25 \u0645\u0644\u063a\u0645.\u0645\u0644-1. \u0648\u0623\u0638\u0647\u0631 \u0627\u062e\u062a\u0628\u0627\u0631 \u062a\u0634\u0643\u064a\u0644 \u0627\u0644\u0645\u0633\u062a\u0639\u0645\u0631\u0627\u062a \u0623\u0646 \u0627\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646 \u0628\u062a\u0631\u0643\u064a\u0632 5 \u0648 10 \u0645\u064a\u0643\u0631\u0648\u0645\u062a\u0631 \u0642\u062f \u062b\u0628\u0637 \u0645\u0639\u0646\u0648\u064a\u0627 \u062a\u0643\u0648\u064a\u0646 \u0645\u0633\u062a\u0639\u0645\u0631\u0627\u062a \u062e\u0644\u0627\u064a\u0627 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a <\/strong>MCF-7 (p<0.01<\/strong>) \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0627\u0644\u0633\u0627\u0644\u0628\u0629.<\/strong><\/span><\/h4>\n

\"\"<\/strong><\/span><\/h4>\n

\"\"<\/strong><\/span><\/h4>\n

\u0644\u0642\u062f \u0628\u064a\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u0625\u0644\u0649 \u0627\u0646 \u062d\u0642\u0646 \u0627\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646 \u0648\u0644\u0645\u062f\u0629 \u0642\u0635\u064a\u0631\u0629 \u0642\u0627\u062f\u0631 \u0639\u0644\u0649 \u062a\u062b\u0628\u064a\u0637 \u0627\u0644\u0627\u0644\u062a\u0647\u0627\u0628 \u0648\u0627\u0644\u0623\u0643\u0633\u062f\u0629 \u0627\u0644\u0646\u0627\u062c\u0645 \u0639\u0646 \u0627\u0644\u0639\u062f\u0648\u0649 \u0648\u0627\u0646 \u0646\u0645\u0648\u0630\u062c \u0627\u0644\u0641\u0626\u0631\u0627\u0646 \u062a\u0634\u064a\u0631 \u0625\u0644\u0649 \u062f\u0648\u0631 \u0627\u0644\u0641\u0627\u0626\u062f\u0629 \u0627\u0644\u0645\u062d\u062a\u0645\u0644\u0629 \u0644\u0644\u0643\u064a\u0648\u0631\u0633\u062a\u064a\u0646 \u0636\u062f \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a.<\/strong><\/span><\/h4>\n
\n

<\/h3>\n

The M.Sc. Thesis discussion of student ( Zainab Abbas AbdulJabar Al-Kubaiasi ) from department of Biology<\/span><\/h3>\n

On Thursday, the 20th of December 2018, at the hall of the late Dr.Salim Abdulhameed,the M.Sc. thesis discussion of student (Zainab Abbas AbdulJabar Al-Kubaiasi) from department of Biology entitled(Evaluation of the effectiveness of quercetin in anti-oxidant, inflammation and cancer cell growth)has been discussed<\/span><\/h3>\n

\u00a0The discussion committee<\/span><\/h3>\n

Prof.Dr.Nahla AbdulRidha Al-Bakri\u00a0\u00a0 Head<\/span><\/h3>\n

Prof.Dr.Ghasan Mohammed Sulaiman\u00a0\u00a0\u00a0 member<\/span><\/h3>\n

Dr.lecturer.Maha Abdulnabi Ghathwan\u00a0\u00a0\u00a0 member<\/span><\/h3>\n

Asst.prof.Dr.Hanadi Salim AbdulSahib\u00a0\u00a0 supervisor member<\/span><\/h3>\n

The Abstract<\/span><\/h3>\n

Flavonoids are considered as promising preventive and therapeutic plant agents against different diseases. Quercetin is one of the most flavonoids found in many fruits and vegetables, such as broccoli, yellow onions, and apples. Quercetin has been shown to reduce proinflammatory molecule expression in vitro and, as well as, exert anti-cancer effects through the inhibition of several intracellular pathways. However, the specific mechanisms behind this type of injury are still not completely understood. This study was undertaken with two main aims. Firstly, to evaluate the ability of quercetin to inhibit lipopolysaccharide (LPS) induced lethal toxicity and proinflammatory molecule and antioxidant expression in vivo. Secondly, the importance of the quercetin as an anti-tumor agent in breast cancer cell line MCF-7. Mice receiving LPS (Salmonella enteriditisLPS, 50 mg\/kg, intraperitoneally) for 90 min after received quercetin for 5 days at dose of 5 and 10 mg kg-1. Sixty male mice were used and divided into 6 groups.\u00a0 In vitro experiments included the effect of quercetin and LPS on the liver and spleen of male mice. In the liver, the enzyme activity was measured by estimating the concentration of malonealdehyde (MDA), glutathione (GSH), and catalase (CAT). While in the spleen, the concentration of cytokines was measured including IL-6, IL-1 beta, IL-33 and TNF-\u03b1. In vitro experiments included MTT assay, clonogenicity test and DAPI + Suforhadamine 101 to assess breast cancer cells apoptosis. The results of organs weight index (liver and spleen) exhibited\u00a0 significant differences (p<0.05) in both 5 and 10 mg kg-1\u00a0 quercetin groups compared to the positive control groups (LPS), and also significant differences were seen between positive and negative control, while there were no significant differences in the positive control group between two studied concentrations of quercetin. The results of glutathione levels showed significant differences (p<0.05) between positive control and other treatments groups. A significant increase was observed due to the treatment of quercetin either individually or in combination with LPS. Moreover, the results showed significant differences between the positive and negative control groups, while quercetin treatment alone did not found any significant differences in comparison to negative control. Levels of malonaldehyde revealed highly significant differences between quercetin treatment groups compared to positive and negative control groups. Additionally, the results showed significant differences in catalase level of in groups treated with quercetin compared to positive control group. There were also significant differences in the positive control group treated with quercetin 5 and 10 mg, while no significant effect was detected in the positive control group in comparison to negative control. Interestingly, the results of IL-6, IL-1\u03b2 and IL-33 levels showed significant differences (p<0.05) in quercetin-treated groups compared with positive control group in dose-dependent manner. Furthermore, this study found significant differences in positive control in comparison to negative control, and significant differences were found within the positive control group depending on the concentration of quercetin. Additionally, TNF-\u03b1 levels showed significant differences (p<0.05) in the groups treated with 5 and 10 mg of quercetin compared to positive control group; however no significant differences were observed in positive group. The cytotoxic effect of quercetin on MCF-7 cancer cell line was examined after the exposure to quercetin for 24 hours using concentrations of 12.5, 25, 50, 100 and 200 \u03bcg\/ml of quercetin. The results showed significant differences at concentration (100, 200) \u03bcg \/ ml compared to control group (p<0.001). There were also significant differences between 200 \u03bcg\/ml with other concentrations (12, 25, 25), and also significant differences were seen between the concentration of 100 \u03bcg\/ml with 25 \u03bcg\/ml at the probability level of p<0.05 and at p<0.01 with concentration of 12.5 \u03bcg\/ml. The findings of this study revealed the inhibition effect quercetin on the growth of the MCF7 cancer cell line that treated with 5 \u03bcg ml-1 of quercetin in comparison to control group.\u00a0 The results of the free radical scavenging activity test for quercetin, known as the DPPH test, showed a significant difference (p<0.05) at concentration of 25 mg. ml-1 compared to 100 mg. ml-1. Also, the same concentration showed significant differences compared to the concentration of 200 mg. ml-1, but at the probability level p<0.01, while the concentration 600 mg. ml-1 showed significant differences at the probability level of p <0.001 in comparison to the concentration of 25 mg. ml-1. Colony formation test showed that quercetin at 5 and 10 \u03bcM of concentrations significantly inhibited the colony formation of MCF-7 breast cancer cells (p<0.01) compared to negative control group<\/span><\/h3>\n

The data implies evidence quercetin injection for short time was capable of inhibiting inflammation and oxidative stress caused by infection and\u00a0 \u00a0mouse model suggest a possible benefit role of quercetin in breast cancer<\/span><\/h3>\n

\u00a0<\/strong><\/span><\/h4>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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