{"id":21628,"date":"2018-12-28T16:34:58","date_gmt":"2018-12-28T13:34:58","guid":{"rendered":"http:\/\/ihcoedu.uobaghdad.edu.iq\/?p=21628"},"modified":"2018-12-30T13:39:06","modified_gmt":"2018-12-30T10:39:06","slug":"%d9%85%d9%86%d8%a7%d9%82%d8%b4%d8%a9-%d8%b1%d8%b3%d8%a7%d9%84%d8%a9-%d8%b7%d8%a7%d9%84%d8%a8%d8%a9-%d8%a7%d9%84%d9%85%d8%a7%d8%ac%d8%b3%d8%aa%d9%8a%d8%b1-%d8%b1%d8%b4%d8%a7-%d8%ad%d8%a8%d9%8a%d8%a8","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=21628","title":{"rendered":"\u0645\u0646\u0627\u0642\u0634\u0629 \u0631\u0633\u0627\u0644\u0629 \u0637\u0627\u0644\u0628\u0629 \u0627\u0644\u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u0631\u0634\u0627 \u062d\u0628\u064a\u0628 \u0641\u0627\u0636\u0644 \u0645\u0646 \u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629"},"content":{"rendered":"

<\/h4>\n

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\"\"<\/h4>\n
\n

The MSc. Thesis discussion of student (Rasha Habib Fadhil) from department of Biology<\/span><\/h3>\n

On Thursday, the 27th of December 2018, the MSc. Thesis discussion of student (Rasha Habib Fadhil) from department of Biology entitled (Evaluation of the Effectiveness of Hesperidin<\/span><\/h3>\n

In anti-oxidant, Inflammation<\/span><\/h3>\n

And Cancer cell growth) has been discussed<\/span><\/h3>\n

The discussion committee<\/span><\/h3>\n

Asst.prof.Dr.Mohammed Mahmood Farhan\u00a0\u00a0 member<\/span><\/h3>\n

Asst.prof.Dr.Zahra’a Hussein Mohammed\u00a0\u00a0 member<\/span><\/h3>\n

Asst.prof.Dr.Jinan Adnan Abdulatif\u00a0\u00a0 member<\/span><\/h3>\n

Asst.prof.Dr.Hanadi Salim Abdulsahib\u00a0\u00a0 supervisor member<\/span><\/h3>\n

The Abstract<\/span><\/h3>\n

Flavonoids are considered as promising preventive and therapeutic plant agents against different diseases. Hesperidin is one of the most flavonoids found in many citrus fruits. Hesperidin has been shown to reduce proinflammatory molecule expression in vitro and, as well as, exert anti-cancer effects through the inhibition of several intracellular pathways. However, the specific mechanisms behind this type of injury are still not completely understood. This study was undertaken with two main aims. Firstly, to evaluate the ability of hesperidin to inhibit lipopolysaccharide (LPS) induced lethal toxicity and proinflammatory molecule and antioxidant expression in vivo. Secondly, the importance of the hesperidin as an anti-tumor agent in breast cancer cell line MCF-7. Mice receiving LPS (Esherichia coli LPS, 50 mg\/kg, intraperitoneally) for 90 min after received hesperidin for 5 days at dose of 5 and 10 mg kg-1. Sixty male mice were used and divided into 6 groups.\u00a0 In vitro experiments included the effect of hesperidin and LPS on the liver and spleen of male mice. In the liver, the enzyme activity was measured by estimating the concentration of malonealdehyde (MDA), glutathione (GSH), and catalase (CAT). While in the spleen, the concentration of cytokines was measured including IL-6, IL-1 beta, IL-33 and TNF-\u03b1. In vitro experiments included MTT assay, clonogenicity test and DAPI + Suforhadamine 101 to assess breast cancer cells apoptosis. The results of organs weight index (liver and spleen) exhibited\u00a0 significant differences (p<0.05) in both 5 and 10 mg kg-1\u00a0 hesperidin groups compared to the positive control groups (LPS), and also significant differences were seen between positive and negative control, while there were no significant differences in the positive control group between two studied concentrations of hesperidin. The results of glutathione levels showed significant differences (p<0.05) between positive control and other treatments groups. A significant increase was observed due to the treatment of hesperidin either individually or in combination with LPS. Moreover, the results showed significant differences between the positive and negative control groups, while hesperidin treatment alone did not found any significant differences in comparison to negative control. Levels of malonaldehyde revealed highly significant differences between hesperidin treatment groups compared to positive and negative control groups. Additionally, the results showed significant differences in catalase level of in groups treated with hesperidin compared to positive control group. There were also significant differences in the positive control group treated with hesperidin 5 and 10 mg, while no significant effect was detected in the positive control group in comparison to negative control. Interestingly, the results of IL-6, IL-1\u03b2 and IL-33 levels showed significant differences (p<0.05) in hesperidin-treated groups compared with positive control group in dose-dependent manner. Furthermore, this study found significant differences in positive control in comparison to egative control, and significant differences were found within the positive control group depending on the concentration of hesperidin. Additionally, TNF-\u03b1 levels showed significant differences (p<0.05) in the groups treated with 5 and 10 mg of hesperidin compared to positive control group; however no significant differences were observed in positive group. The cytotoxic effect of hesperidin on MCF-7 cancer cell line was examined after the exposure to hespeidin for 24 hours using concentrations of 12.5, 25, 50, 100 and 200 \u03bcg\/ml of hesperdien. The results showed significant differences at concentration (100, 200) \u03bcg \/ ml compared to control group (p<0.001). There were also significant differences between the of 200 \u03bcg\/ml with other concentrations (12, 5, 25), and also significant differences were seen between the concentration of 100 \u03bcg\/ml with 25 \u03bcg\/ml at the probability level of p<0.05 and at p<0.01 with concentration of 12.5 \u03bcg\/ml. The findings of this study revealed the inhibition effect hesperidin on the growth of the MCF7 cancer cell line that treated with 5 \u03bcg ml-1 of hesperidin in comparison to control group.\u00a0 The results of the free radical scavenging activity test for hesperidin, known as the DPPH test, showed a significant difference (p<0.05) at concentration of 10 mg. ml-1 compared to 40 mg. ml-1. Also, the same concentration showed significant differences compared to the concentration of 60 mg. ml-1, but at the probability level p<0.01, while the concentration 100 mg. ml-1 showed significant differences at the probability level of p <0.001 in comparison to the concentration of 10\u00a0 mg. ml-1.colony formation test showed that hesperidin at 20 and 40 \u03bcM of concentrations significantly inhibited the colony formation of MCF-7 breast cancer cells (p<0.01) compared to negative control group.<\/span><\/h3>\n

The data implies evidence hesperidin injection for short time was capable of inhibiting inflammation and oxidative stress caused by infection and inhibiting Colony formation in MCF-7 breast cancer cells a possible benefit role of hesperidin as a preventive treatment<\/span><\/h3>\n

<\/h3>\n

<\/h3>\n","protected":false},"excerpt":{"rendered":"

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