{"id":28221,"date":"2019-11-13T00:47:31","date_gmt":"2019-11-12T21:47:31","guid":{"rendered":"http:\/\/ihcoedu.uobaghdad.edu.iq\/?p=28221"},"modified":"2020-02-13T12:39:35","modified_gmt":"2020-02-13T09:39:35","slug":"%d9%82%d8%b3%d9%85-%d8%b9%d9%84%d9%88%d9%85-%d8%a7%d9%84%d8%ad%d9%8a%d8%a7%d8%a9-%d9%8a%d9%86%d8%a7%d9%82%d8%b4-%d8%b1%d8%b3%d8%a7%d9%84%d8%a9-%d9%85%d8%a7%d8%ac%d8%b3%d8%aa%d9%8a%d8%b1-%d8%aa%d8%af-3","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=28221","title":{"rendered":"\u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u064a\u0646\u0627\u0642\u0634 \u0631\u0633\u0627\u0644\u0629 \u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u062a\u062f\u0631\u0633 \u062a\u0623\u062b\u064a\u0631 \u0628\u0639\u0636 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 \u0639\u0644\u0649 \u0628\u0643\u062a\u0631\u064a\u0627 \u00a0Cronobacter sakazakii"},"content":{"rendered":"

\u0646\u0627\u0642\u0634 \u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u062a\u0631\u0628\u064a\u0629 \u0644\u0644\u0639\u0644\u0648\u0645 \u0627\u0644\u0635\u0631\u0641\u0629 (\u0627\u0628\u0646 \u0627\u0644\u0647\u064a\u062b\u0645) \u0631\u0633\u0627\u0644\u0629 \u0627\u0644\u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u0627\u0644\u0645\u0648\u0633\u0648\u0645\u0629 (\u062f\u0631\u0627\u0633\u0629 \u0645\u0638\u0647\u0631\u064a\u0629 \u0648\u062c\u0632\u064a\u0626\u064a\u0629 \u0644\u062a\u0623\u062b\u064a\u0631 \u0628\u0639\u0636 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 \u0639\u0644\u0649 \u0628\u0643\u062a\u0631\u064a\u0627 \u00a0Cronobacter sakazakii) \u0644\u0644\u0637\u0627\u0644\u0628\u0629 (\u0643\u0627\u0631\u062f\u064a\u0646\u064a\u0627 \u0639\u0627\u0645\u0631 \u0627\u0633\u0645\u0627\u0639\u064a\u0644 ) \u0627\u0644\u062a\u064a \u0627\u0646\u062c\u0632\u062a\u0647\u0627 \u0628\u0627\u0634\u0631\u0627\u0641 \u0627\u0644\u062a\u062f\u0631\u064a\u0633\u064a\u0629 \u0641\u064a \u0627\u0644\u0642\u0633\u0645 (\u0627.\u0645.\u062f. \u0644\u0645\u0649 \u0639\u0628\u062f \u0627\u0644\u0647\u0627\u062f\u064a \u0632\u0648\u064a\u0646).<\/span><\/h4>\n

\"\"<\/span><\/h4>\n

\u0623\u062c\u0631\u064a\u062a \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0644\u062a\u062b\u0628\u064a\u0637 \u0646\u0645\u0648 \u0628\u0643\u062a\u0631\u064a\u0627 Cronobacter \u00a0sakazakii\u00a0 \u00a0\u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0645\u0631\u0643\u0628\u0627\u062a Citral) \u060c Curcumin\u060cInulin \u060cResveratrol \u060c Trans-cinnamaldhyde)\u060c \u0627\u0630 \u062a\u0645 \u0627\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 17 \u0639\u0632\u0644\u0629 \u0645\u0646 \u0645\u0635\u0627\u062f\u0631 \u0633\u0627\u0628\u0642\u0629\u060c \u0627\u0630 \u0632\u0631\u0639\u062a \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0639\u0644\u0649 \u0648\u0633\u0637 \u00a0\u0627\u0644\u0627\u0643\u0627\u0631 \u0627\u0644\u0645\u0627\u0643\u0648\u0646\u0643\u064a \u0648\u0648\u0633\u0637 \u0627\u0644\u062a\u0631\u0628\u062a\u0648\u0646 \u0635\u0648\u064a\u0627 \u0627\u0644\u0635\u0644\u0628 \u062b\u0645 \u0634\u062e\u0635\u062a \u062c\u0645\u064a\u0639 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062c\u064a\u0646 \u0627\u0644\u062a\u0634\u062e\u064a\u063516S rRNA \u00a0\u0648\u0643\u0627\u0646\u062a \u062c\u0645\u064a\u0639\u0647\u0627 \u062a\u0639\u0648\u062f \u0644\u0628\u0643\u062a\u0631\u064a\u0627C. sakazakii \u00a0\u0648\u062a\u0636\u0645\u0646\u062a 6 \u0639\u0632\u0644\u0627\u062a \u0645\u0646 \u0639\u064a\u0646\u0627\u062a \u0627\u0644\u062f\u0645 \u00a0Blood (B)\u06486 \u0639\u0632\u0644\u0627\u062a \u0645\u0646 \u0633\u0627\u0626\u0644 \u0627\u0644\u0646\u062e\u0627\u0639 \u0627\u0644\u0634\u0648\u0643\u064a Cerobrospinal fluid (CSF) \u00a0\u06483 \u0639\u0632\u0644\u0627\u062a \u0645\u0646 \u0639\u064a\u0646\u0627\u062a \u062d\u0644\u064a\u0628 Dialak (A1) \u00a0\u0648\u0639\u0632\u0644\u0629 \u0648\u0627\u062d\u062f\u0629 \u0645\u0646 \u062d\u0644\u064a\u0628 Novolac Allernova (C1) \u0648\u0639\u0632\u0644\u0629 \u0648\u0627\u062d\u062f\u0629 \u0645\u0646 \u0639\u064a\u0646\u0629 \u0627\u0644\u062d\u0644\u064a\u0628Novolac AD (C2).<\/span><\/h4>\n

\u0628\u064a\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062d\u0631\u064a \u0639\u0646 \u0628\u0639\u0636 \u0639\u0648\u0627\u0645\u0644 \u0627\u0644\u0636\u0631\u0627\u0648\u0629 \u0644\u0628\u0643\u062a\u0631\u064a\u0627C. sakazakii \u00a0\u0645\u0638\u0647\u0631\u064a\u0627\u064b\u060c \u0627\u0646 \u062c\u0645\u064a\u0639 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0644\u0647\u0627 \u0627\u0644\u0642\u062f\u0631\u0629 \u0639\u0644\u0649 \u0627\u0644\u062d\u0631\u0643\u0629 Motility \u00a0\u060c \u0643\u0645\u0627 \u0623\u0638\u0647\u0631\u062a \u062c\u0645\u064a\u0639 \u0639\u0632\u0644\u0627\u062a \u0628\u0643\u062a\u0631\u064a\u0627 C. sakazakii \u00a0\u0642\u062f\u0631\u062a\u0647\u0627 \u0639\u0644\u0649 \u0627\u062d\u062f\u0627\u062b \u062d\u0631\u0643\u0629 \u0627\u0644\u0633\u0628\u0627\u062d\u0629 Swimming \u00a0\u0628\u0642\u0637\u0631 \u064a\u062a\u0631\u0627\u0648\u062d (17-79) \u0645\u0644\u0645\u060c \u0648\u062a\u0643\u0648\u064a\u0646 \u0638\u0627\u0647\u0631\u0629 \u0627\u0644\u0627\u0646\u062b\u064a\u0627\u0644Swarming \u00a0\u0628\u0642\u0637\u0631 \u064a\u062a\u0631\u0627\u0648\u062d (4-46) \u0645\u0644\u0645\u060c \u0643\u0645\u0627 \u0644\u0648\u062d\u0638 \u0642\u062f\u0631\u062a\u0647\u0627 \u0639\u0644\u0649 \u0627\u0646\u062a\u0627\u062c \u0627\u0646\u0632\u064a\u0645 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0632 \u0628\u0642\u0637\u0631 \u064a\u062a\u0631\u0627\u0648\u062d (8-30) \u0645\u0644\u0645\u060c \u0648 \u0642\u062f\u0631\u062a\u0647\u0627 \u0639\u0644\u0649 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0628\u0637\u0631\u064a\u0642\u0629 Microtiter Plate .<\/span><\/h4>\n

\u062f\u064f\u0631\u0650\u0633 \u062a\u0623\u062b\u064a\u0631 \u0643\u0644 \u0645\u0646 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 (Citral\u060cCurcumin \u060cInulin \u060c Resveratrol\u060c Trans-cinnamaldhyde) \u0641\u064a \u00a0\u0646\u0645\u0648 \u0628\u0643\u062a\u0631\u064a\u0627C. sakazakii\u00a0 \u00a0\u00a0\u0644\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0645\u0646\u062a\u062e\u0628\u0629 (A1b\u060c B4\u060c CSF4) \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0643\u0644 \u0645\u0646 \u00a0\u0627\u0644\u062a\u0631\u0643\u064a\u0632 (1\u060c 10\u060c 100)\u0645\u0627\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645 \u00a0\u0641\u0642\u062f \u0623\u0638\u0647\u0631\u062a \u062c\u0645\u064a\u0639 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0641\u0639\u0644\u0627\u064b \u062a\u062b\u0628\u064a\u0637\u064a\u0627\u064b \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0639\u0627\u0644\u064a\u0629\u060c \u0627\u0645\u0627 \u0645\u0631\u0643\u0628Trans-cinnamaldhyde \u00a0\u0641\u0642\u062f \u0627\u0638\u0647\u0631 \u0641\u0639\u0644\u0627\u064b \u062a\u062b\u0628\u064a\u0637\u064a\u0627\u064b \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0645\u0646\u062e\u0641\u0636\u0629 \u0648\u0627\u0644\u0639\u0627\u0644\u064a\u0629.<\/span><\/h4>\n

\u062a\u0645 \u062f\u0631\u0627\u0633\u0629 \u062a\u0623\u062b\u064a\u0631 \u0643\u0644 \u0645\u0646 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 (Citral\u060cCurcumin \u060c Inulin \u060cResveratrol \u060c Trans-cinnamaldhyde) \u0641\u064a \u0638\u0627\u0647\u0631\u062a\u064a \u0627\u0644\u0633\u0628\u0627\u062d\u0629Swimming \u00a0\u0648\u0627\u0644\u0627\u0646\u062b\u064a\u0627\u0644<\/span><\/h4>\n

Swarming \u00a0\u0639\u0644\u0649 \u0643\u0644 \u0645\u0646 \u0627\u0644\u0639\u0632\u0644\u0629A1b) \u060c B4 \u060c CSF4) \u0641\u0642\u062f \u0623\u0638\u0647\u0631\u062a \u0643\u0644 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u062a\u0623\u062b\u064a\u0631\u0627\u064b \u062a\u062b\u0628\u064a\u0637\u064a\u0627\u064b\u00a0\u00a0 \u0648\u0628\u0646\u0633\u0628 \u0645\u062a\u0641\u0627\u0648\u062a\u0629 \u0627\u0639\u062a\u0645\u0627\u062f\u0627\u064b \u0639\u0644\u0649 \u0646\u0648\u0639 \u0627\u0644\u0645\u0631\u0643\u0628 \u0648\u062a\u0631\u0643\u064a\u0632\u0647.<\/span><\/h4>\n

\u0643\u0645\u0627 \u062f\u0631\u0633 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 \u0641\u064a \u0639\u0648\u0627\u0645\u0644 \u0636\u0631\u0627\u0648\u0629 \u0623\u062e\u0631\u0649 \u0644\u0628\u0643\u062a\u0631\u064a\u0627C. sakazakii \u00a0\u0645\u062b\u0644 \u0627\u0646\u062a\u0627\u062c \u0627\u0646\u0632\u064a\u0645 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0632 \u0641\u064a \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0645\u0646\u062a\u062e\u0628\u0629\u060c \u0641\u0642\u062f \u0627\u0638\u0647\u0631 \u062a\u0623\u062b\u064a\u0631 \u0645\u0631\u0643\u0628 Trans-cinnamaldhyde \u0641\u0639\u0644\u0627\u064b \u062a\u062b\u0628\u064a\u0637\u064a\u0627\u064b \u0644\u0627\u0646\u062a\u0627\u062c \u0627\u0646\u0632\u064a\u0645 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0632\u00a0 \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0643\u064a\u0632 100 \u0645\u0627\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645 \u0628\u0646\u0633\u0628\u0629 100% \u0641\u064a \u0643\u0644 \u0645\u0646 \u0627\u0644\u0639\u0632\u0644\u0629 A1b\u060c B4\u060c CSF4 \u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0628\u0645\u0631\u0643\u0628Citral \u060cInulin \u00a0\u0625\u0630 \u0644\u0648\u062d\u0638 \u062a\u0623\u062b\u064a\u0631 \u0645\u062a\u0648\u0633\u0637 \u0627\u0645\u0627 \u00a0\u0645\u0631\u0643\u0628 Curcumin \u0648 Resveratrol \u0641\u0642\u062f \u0643\u0627\u0646 \u0645\u0646\u062e\u0641\u0636\u0627\u064b \u00a0\u0641\u064a \u0643\u0644 \u0645\u0646\u00a0 \u0627\u0644\u0639\u0632\u0644\u0629 A1b\u060c B4\u060c \u0627\u0645\u0627 \u0641\u064a \u00a0\u0627\u0644\u0639\u0632\u0644\u0629 CSF4 \u0641\u0642\u062f \u0644\u0648\u062d\u0638 \u062a\u0623\u062b\u064a\u0631 \u0645\u0631\u0643\u0628 (Citral\u060c Curcumin\u060c Inulin \u060c Resveratrol) \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0643\u064a\u0632 100\u0645\u0627\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\u00a0 \u0627\u0643\u062b\u0631 \u0645\u0646 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 50 \u0645\u0627\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645 \u00a0\u0648\u0643\u0627\u0646 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 \u0641\u064a \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a Biofilm \u00a0\u0644\u0639\u0632\u0644\u0627\u062a \u0628\u0643\u062a\u0631\u064a\u0627C. sakazakii \u00a0\u0641\u0642\u062f \u0623\u0638\u0647\u0631\u062a \u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0641\u064a \u062a\u062b\u0628\u064a\u0637 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0645\u0646\u062e\u0641\u0636\u0629 \u0648\u0627\u0644\u0639\u0627\u0644\u064a\u0629.<\/span><\/h4>\n

\u062f\u0631\u0633 \u062a\u0623\u062b\u064a\u0631 \u062f\u0631\u062c\u0627\u062a \u0627\u0644\u062d\u0631\u0627\u0631\u0629 \u0627\u0644\u0645\u062e\u062a\u0644\u0641\u0629 \u0641\u064a \u0646\u0645\u0648 \u0628\u0643\u062a\u0631\u064a\u0627 C. sakazakii \u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u062f\u0645 \u0648\u0633\u0627\u0626\u0644 \u0627\u0644\u0646\u062e\u0627\u0639 \u0627\u0644\u0634\u0648\u0643\u064a \u0648\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u062d\u0644\u064a\u0628 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062c\u0647\u0627\u0632Spectrophotometer \u00a0\u0627\u0630 \u0623\u0638\u0647\u0631\u062a \u062f\u0631\u062c\u0629 \u0627\u0644\u062d\u0631\u0627\u0631\u0629 55\u00ba\u0645 \u0627\u0646\u062e\u0641\u0627\u0636\u0627\u064b \u0641\u064a \u0646\u0645\u0648 \u0627\u0644\u0628\u0643\u062a\u0631\u064a\u0627 \u00a0\u00a0\u0648\u0642\u062f \u062a\u0631\u0627\u0648\u062d\u062a (0.02-0.21) \u00a0\u0627\u0645\u0627 \u0639\u0646\u062f \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 25\u00ba\u0645 \u0641\u0642\u062f \u062a\u0631\u0627\u0648\u062d\u062a (0.14-0.31) \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u062f\u0631\u062c\u0629 37 \u0645\u00ba \u0627\u0644\u062a\u064a \u062a\u0631\u0627\u0648\u062d\u062a (0.38-0.66).<\/span><\/h4>\n

\u062f\u0631\u0633 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0627\u0633 \u0627\u0644\u0647\u064a\u062f\u0631\u0648\u062c\u064a\u0646\u064a pH \u00a0\u0641\u064a \u0646\u0645\u0648 \u0628\u0643\u062a\u0631\u064a\u0627 C. sakazakii \u00a0\u0641\u064a \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0645\u0646\u062a\u062e\u0628\u0629 \u00a0\u00a0\u0641\u0642\u062f \u0644\u0648\u062d\u0638 \u0627\u0646\u062e\u0641\u0627\u0636 \u0646\u0645\u0648 \u0627\u0644\u0628\u0643\u062a\u0631\u064a\u0627 \u0639\u0646\u062f \u0627\u0644\u0627\u0633 \u0627\u0644\u0647\u064a\u062f\u0631\u0648\u062c\u064a\u0646\u064a 4 \u0627\u0630 \u0643\u0627\u0646 \u064a\u062a\u0631\u0627\u0648\u062d (0.02- 0.29)\u060c \u0627\u0645\u0627 \u0639\u0646\u062f \u0627\u0644\u0627\u0633 \u0627\u0644\u0647\u064a\u062f\u0631\u0648\u062c\u064a\u0646\u064a \u0627\u0644\u0642\u0627\u0639\u062f\u064a \u00a09\u0641\u0642\u062f \u0628\u064a\u0646\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u00a0\u0627\u0631\u062a\u0641\u0627\u0639 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a \u0641\u0642\u062f \u062a\u0631\u0627\u0648\u062d (0.05-0.67) \u00a0\u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0627\u0644\u0627\u0633 \u0627\u0644\u0647\u064a\u062f\u0631\u0648\u062c\u064a\u0646\u064a \u0627\u0644\u0645\u062a\u0639\u0627\u062f\u0644\u00a0 \u0627\u0630 \u062a\u0631\u0627\u0648\u062d (0.29-1.42).<\/span><\/h4>\n

\u062a\u0645 \u0627\u0644\u062a\u062d\u0631\u064a \u0639\u0646 \u0628\u0639\u0636 \u062c\u064a\u0646\u0627\u062a \u0627\u0644\u0636\u0631\u0627\u0648\u0629 \u0644\u0628\u0643\u062a\u0631\u064a\u0627 C. sakazakii \u0648\u062a\u0636\u0645\u0646\u062a \u0647\u0630\u0647 \u0627\u0644\u062c\u064a\u0646\u0627\u062a \u0643\u0644\u0627\u064b \u0645\u0646\u00a0<\/span>zpx \u0627\u0644\u0630\u064a \u064a\u0634\u0641\u0631 \u0627\u0644\u0649 \u0627\u0646\u062a\u0627\u062c \u0627\u0646\u0632\u064a\u0645 Proteases \u0648\u062c\u064a\u0646 flgJ \u0627\u0644\u0630\u064a \u064a\u0634\u0641\u0631 \u0627\u0644\u0649 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u0633\u0648\u0637 \u0648\u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0648\u062c\u0648\u062f \u0647\u0630\u0647 \u0627\u0644\u062c\u064a\u0646\u0627\u062a \u0628\u0646\u0633\u0628\u0629 100% \u0641\u064a \u0627\u0644\u0639\u0632\u0644\u0629 CSF4 .<\/span><\/h4>\n

\u062f\u0631\u0633 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 \u0641\u064a \u0645\u0642\u062f\u0627\u0631 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u062c\u064a\u0646 flgJ\u060c \u0627\u0630 \u062a\u0634\u064a\u0631 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0649 \u0648\u062c\u0648\u062f \u062a\u0628\u0627\u064a\u0646 \u0641\u064a \u0645\u0633\u062a\u0648\u064a\u0627\u062a \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 flgJ \u0628\u0639\u062f \u0645\u0639\u0627\u0645\u0644\u0629 \u0628\u0643\u062a\u0631\u064a\u0627sakazakii . C \u0641\u064a \u0627\u0644\u0639\u0632\u0644\u0629 CSF4 \u060c \u0627\u0630 \u0627\u062f\u0649 \u0645\u0631\u0643\u0628Curcumin \u00a0\u0628\u062a\u0631\u0643\u064a\u0632 Sub minimum inhibation concentration (SMIC) \u00a0(\u0627\u0646\u062a\u062e\u0628 \u0639\u0644\u0649 \u0627\u0633\u0627\u0633 \u062a\u062c\u0627\u0631\u0628 \u0645\u0646\u0641\u0631\u062f\u0629) \u0627\u0644\u0649 \u062e\u0641\u0636 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0627\u0644\u0649 0.2Folding Change \u00a0\u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0645\u0639 \u0645\u0639\u0627\u0645\u0644\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0627\u0645\u0627 \u0645\u0631\u0643\u0628 (Citral\u060c Inulin\u060c \u00a0Resveratrol \u00a0Trans-cinnamaldhyde). \u0641\u0642\u062f \u0623\u062f\u0649 \u0627\u0644\u0649 \u0632\u064a\u0627\u062f\u0629\u00a0 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 flgJ \u0644\u062a\u0628\u0644\u063a\u00a0 (12.6 \u060c 20.7\u060c 19.4\u060c 6.3) Folding Change \u0639\u0644\u0649 \u0627\u0644\u062a\u0648\u0627\u0644\u064a \u00a0\u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0645\u0639 \u0645\u0639\u0627\u0645\u0644\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629.<\/span><\/h4>\n

\"\"<\/h4>\n

\u062a\u0645 \u0641\u064a \u0647\u0630\u0647\u0650 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 \u0641\u064a \u0645\u0642\u062f\u0627\u0631 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u062c\u064a\u0646 zpx\u060c \u0648\u0642\u062f \u0623\u0634\u0627\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0649 \u0648\u062c\u0648\u062f \u062a\u0628\u0627\u064a\u0646 \u0641\u064a \u0645\u0633\u062a\u0648\u064a\u0627\u062a \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 zpx \u0628\u0639\u062f \u0645\u0639\u0627\u0645\u0644\u0629 \u0628\u0643\u062a\u0631\u064a\u0627 C. sakazakii\u00a0 \u0644\u0644\u0639\u0632\u0644\u0629 (CSF4)\u060c \u0627\u0630 \u0623\u062f\u0649 \u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0643\u0644 \u0645\u0646 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a Citral) \u060c Curcumin\u060c Inulin\u060c Resveratrol\u060c Trans-cinnamaldhyde) \u00a0\u0628\u062a\u0631\u0643\u064a\u0632 \u00a0Sub minimum inhibition concentration (SMIC) \u0627\u0644\u0649 \u0632\u064a\u0627\u062f\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 zpx \u0644\u064a\u0628\u0644\u063a (21.3\u060c 18.7\u060c 29.9\u060c 34.1\u060c 10.9) Folding Change \u0639\u0644\u0649 \u0627\u0644\u062a\u0648\u0627\u0644\u064a \u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0628\u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629.<\/span><\/h4>\n

\u062f\u0631\u0633 \u062a\u0623\u062b\u064a\u0631 \u0645\u0631\u0643\u0628 Trance-cinnamaldhyde \u0641\u064a \u0645\u0642\u062f\u0627\u0631 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u062c\u064a\u0646 flgJ \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0648\u0633\u0637 Swimming \u00a0\u0648Swarming \u0627\u0630 \u062a\u0634\u064a\u0631 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0649 \u0648\u062c\u0648\u062f \u0641\u0631\u0648\u0642\u0627\u062a \u0641\u064a \u0645\u0633\u062a\u0648\u064a\u0627\u062a \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u062c\u064a\u0646 flgJ \u0628\u0639\u062f \u0645\u0639\u0627\u0645\u0644\u0629 \u0628\u0643\u062a\u0631\u064a\u0627C. Sakazakii \u00a0\u0644\u0644\u0639\u0632\u0644\u0629CSF4 \u00a0\u0627\u0644\u0645\u0646\u062b\u0627\u0644\u0629 \u0628\u0645\u0631\u0643\u0628 Trance-cinnamaldhyde \u0628\u062a\u0631\u0643\u064a\u0632( SMIC )Sub minimum inhibation concentration \u00a0\u060c \u0641\u0642\u062f \u0644\u0648\u062d\u0638 \u00a0\u0627\u0646\u062e\u0641\u0627\u0636 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 flgJ \u00a0\u0644\u0644\u0639\u0632\u0644\u0629 \u0627\u0644\u0645\u0646\u062b\u0627\u0644\u0629 \u0627\u0644\u0649 0.14 Folding change \u00a0\u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0645\u0639 \u0645\u0639\u0627\u0645\u0644\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629\u00a0 \u0641\u064a \u062d\u064a\u0646 \u0643\u0627\u0646 \u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0645\u0631\u0643\u0628 Trance-cinnamaldhyde \u0628\u062a\u0631\u0643\u064a\u0632 SMIC \u0623\u062f\u0649 \u0627\u0644\u0649 \u0632\u064a\u0627\u062f\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 flgJ \u0641\u064a \u0627\u0644\u0639\u0632\u0644\u0629 \u0627\u0644\u0633\u0627\u0628\u062d\u0629 \u0627\u0644\u0649 13.65Folding Change \u00a0\u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0645\u0639\u0627\u0645\u0644\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629.<\/span><\/h4>\n

\u0627\u0645\u0627 \u062a\u0623\u062b\u064a\u0631 \u0645\u0631\u0643\u0628 Trance-cinnamaldhyde \u0641\u064a \u0645\u0642\u062f\u0627\u0631 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u062c\u064a\u0646 zpx \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0648\u0633\u0637 Swarming \u00a0\u0648Swimming \u00a0\u0641\u0642\u062f \u0627\u0634\u0627\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0649 \u0638\u0647\u0648\u0631 \u0645\u0633\u062a\u0648\u064a\u0627\u062a \u0645\u062a\u0628\u0627\u064a\u0646\u0629 \u0641\u064a \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 zpx \u0628\u0639\u062f \u0645\u0639\u0627\u0645\u0644\u0629 \u0628\u0643\u062a\u0631\u064a\u0627C. sakazakii \u00a0\u00a0\u0644\u0644\u0639\u0632\u0644\u0629CSF4 \u00a0\u0627\u0630 \u0623\u062f\u0649 \u0627\u0644\u0649 \u0632\u064a\u0627\u062f\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 zpx \u0641\u064a \u0638\u0627\u0647\u0631\u0629 Swarming \u0639\u0646\u062f \u0627\u0633\u062a\u062e\u062f\u0627\u0645\u00a0 \u062a\u0631\u0643\u064a\u0632 Sub minimum inhibation concentration SMIC)) \u0644\u062a\u0635\u0644 \u0627\u0644\u0649 3.0Folding change \u00a0\u0641\u064a \u0627\u0644\u0639\u0632\u0644\u0629 \u0627\u0644\u0645\u0646\u062b\u0627\u0644\u0629 \u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0645\u0639 \u0645\u0639\u0627\u0645\u0644\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0627\u0645\u0627 \u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062a\u0631\u0643\u064a\u0632 SMIC \u0645\u0646 \u0645\u0631\u0643\u0628 Trance-cinnamaldhyde \u0623\u062f\u0649 \u0627\u0644\u0649 \u0632\u064a\u0627\u062f\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u00a0\u062c\u064a\u0646 zpx \u0641\u064a \u0627\u0644\u0639\u0632\u0644\u0629 \u0627\u0644\u0633\u0627\u0628\u062d\u0629 \u0627\u0644\u0649 44.8 folding change \u00a0\u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0628\u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u0633\u064a\u0637\u0631\u0629.<\/span><\/h4>\n

<\/h4>\n

\u0648\u0623\u0638\u0647\u0631\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0642\u062f\u0631\u0629 \u0627\u0644\u0628\u0643\u062a\u0631\u064a\u0627C.sakazakii\u00a0 \u00a0\u0639\u0644\u0649 \u0627\u0644\u062d\u0631\u0643\u0629 \u0648\u0627\u062d\u062f\u0627\u062b \u0638\u0627\u0647\u0631\u062a\u064a \u0627\u0644\u0627\u0646\u062b\u064a\u0627\u0644 Swarming \u00a0\u0648\u0627\u0644\u0633\u0628\u0627\u062d\u0629 Swimming \u00a0\u0641\u0636\u0644\u0627\u064b \u0639\u0646 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a Biofilm \u00a0\u0648\u0625\u0646\u062a\u0627\u062c \u0627\u0646\u0632\u064a\u0645 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0632 Protease.<\/span><\/h4>\n

\u0623\u0638\u0647\u0631\u062a \u0642\u062f\u0631\u0629 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 (Citral\u060cCurcumin \u060cInulin \u060c Resveratrol) \u00a0\u0641\u0639\u0627\u0644\u064a\u062a\u0647\u0627 \u0641\u064a \u062a\u062b\u0628\u064a\u0637 \u0646\u0645\u0648 \u0628\u0643\u062a\u0631\u064a\u0627 C. sakazakii \u00a0\u0648\u0628\u0646\u0633\u0628 \u0645\u062a\u0641\u0627\u0648\u062a\u0629 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0645\u0631\u062a\u0641\u0639\u0629 \u060c \u0627\u0645\u0627 \u0645\u0631\u0643\u0628 Trans-cinnamaldhyde \u00a0\u0641\u0642\u062f \u0623\u0638\u0647\u0631\u062a \u0643\u0641\u0627\u0621\u062a\u0647 \u0641\u064a \u062a\u062b\u0628\u064a\u0637 \u0646\u0645\u0648 \u0627\u0644\u0628\u0643\u062a\u0631\u064a\u0627 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0639\u0627\u0644\u064a\u0629 \u0648\u0627\u0644\u0645\u0646\u062e\u0641\u0636\u0629.<\/span><\/h4>\n

\u0623\u0638\u0647\u0631\u062a \u0643\u0641\u0627\u0621\u0629 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 (Citral\u060cCurcumin \u060cInulin \u060c Resveratrol \u060c Trans-cinnamaldhyde) \u0641\u064a \u062a\u062b\u0628\u064a\u0637 \u0638\u0627\u0647\u0631\u0629 \u0627\u0644\u0633\u0628\u0627\u062d\u0629 \u0627\u0643\u062b\u0631 \u0645\u0646 \u0638\u0627\u0647\u0631\u0629 \u0627\u0644\u0627\u0646\u062b\u064a\u0627\u0644.<\/span><\/h4>\n

\u0623\u0638\u0647\u0631\u062a \u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a (Trans-cinnamaldhyde\u060c Citral \u060c Inulin) \u0641\u064a \u062a\u062b\u0628\u064a\u0637\u00a0 \u0628\u0639\u0636 \u0639\u0648\u0627\u0645\u0644 \u0627\u0644\u0636\u0631\u0627\u0648\u0629 (\u0627\u0646\u062a\u0627\u062c \u0627\u0646\u0632\u064a\u0645 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0632 \u0648\u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a) \u0627\u0643\u062b\u0631 \u0645\u0646 \u0645\u0631\u0643\u0628 Curcumin \u00a0\u0648.Resveratrol<\/span><\/h4>\n

\u062a\u0623\u062b\u0631 \u0646\u0645\u0648 \u00a0\u0628\u0643\u062a\u0631\u064a\u0627C. sakazakii\u00a0 \u00a0\u0639\u0646\u062f \u062f\u0631\u062c\u0627\u062a \u0627\u0644\u062d\u0631\u0627\u0631\u0629 \u0627\u0644\u0639\u0627\u0644\u064a\u0629 \u0648\u0627\u0644\u0645\u0646\u062e\u0641\u0636\u0629 \u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0645\u0639 \u062f\u0631\u062c\u0629 \u0627\u0644\u062d\u0631\u0627\u0631\u0629 \u0627\u0644\u0645\u062b\u0644\u0649 37 \u00ba\u0645.<\/span><\/h4>\n

\u062a\u0623\u062b\u0631 \u0646\u0645\u0648 \u00a0\u0628\u0643\u062a\u0631\u064a\u0627 C. sakazakii \u00a0\u0641\u064a \u0627\u0644\u0648\u0633\u0637 \u0627\u0644\u062d\u0627\u0645\u0636\u064a \u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0628\u0627\u0644\u0627\u0633 \u0627\u0644\u0647\u064a\u062f\u0631\u0648\u062c\u064a\u0646\u064a \u0627\u0644\u0645\u062a\u0639\u0627\u062f\u0644 \u0648\u0627\u0644\u0642\u0627\u0639\u062f\u064a.<\/span><\/h4>\n

\u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0643\u0634\u0641 \u0639\u0646 \u062c\u064a\u0646\u0627\u062a \u0627\u0644\u0636\u0631\u0627\u0648\u0629 \u0627\u0645\u062a\u0644\u0627\u0643 \u0628\u0643\u062a\u0631\u064a\u0627C .sakazakii\u00a0 \u00a0\u0643\u0644 \u0645\u0646 \u062c\u064a\u0646 zpx \u00a0\u0648flgJ \u00a0\u0648\u0628\u0646\u0633\u0628\u0629 100%.<\/span><\/h4>\n

\u0623\u0638\u0647\u0631\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u062a\u062d\u062a \u0627\u0644\u0645\u062b\u0628\u0637 SMIC \u00a0\u0644\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 (Citral\u060c Curcumin \u060c Inulin \u060c Resveratrol \u060c Trans-cinnamaldhyde) \u0641\u064a \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0643\u0644 \u0645\u0646 \u0627\u0644\u062c\u064a\u0646 flgJ \u0648zpx \u00a0\u00a0\u0641\u064a \u0627\u0644\u0639\u0632\u0644\u0629 ((CSF4 \u0648 \u0632\u064a\u0627\u062f\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 zpx.<\/span><\/h4>\n

\u0623\u0638\u0647\u0631\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u00a0\u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u062a\u0623\u062b\u064a\u0631 \u0645\u0631\u0643\u0628 Trans-cinnamaldhyde\u0641\u064a \u0645\u0633\u062a\u0648\u0649 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0643\u0644 \u0645\u0646 \u062c\u064a\u0646flgJ \u00a0\u0648 zpx\u00a0 \u0641\u064a \u0638\u0627\u0647\u0631\u062a\u064a \u0627\u0644\u0633\u0628\u0627\u062d\u0629 Swimming \u00a0\u0648\u0627\u0644\u0627\u0646\u062b\u064a\u0627\u0644 Swarming \u00a0\u0641\u0642\u062f \u0643\u0627\u0646 \u0644\u062a\u0623\u062b\u064a\u0631 \u0645\u0631\u0643\u0628 Trans-cinnamaldhyde \u062f\u0648\u0631 \u0641\u064a \u062a\u0642\u0644\u064a\u0644 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u062c\u064a\u0646 flgJ \u0627\u0643\u062b\u0631 \u0645\u0646 \u0627\u0644\u062c\u064a\u0646 zpx \u00a0\u060c \u0643\u0645\u0627 \u0643\u0627\u0646 \u062a\u0623\u062b\u064a\u0631\u0647 \u0641\u064a \u0638\u0627\u0647\u0631\u0629 \u0627\u0644\u0627\u0646\u062b\u064a\u0627\u0644 \u0627\u0643\u062b\u0631 \u0645\u0646 \u0638\u0627\u0647\u0631\u0629 \u0627\u0644\u0633\u0628\u0627\u062d\u0629.<\/span><\/h4>\n
\n

Morphology and Molecular\u00a0 Study of Effect\u00a0 Natural Compounds on\u00a0 Cronobacter sakazakii <\/span><\/h3>\n

By Gardinea Amir Ismail<\/span><\/h3>\n

Supervised by Assist-Professor Dr- Luma Abd alhadi Zewien<\/span><\/h3>\n

Abstract<\/span><\/h3>\n

In the present study aimed to detect the effect (Citral,Curcumin, Inulin,Resveratrol,Trans-cinnamaldhyde) compound on C. Sakazakii growth. Seventeen isolate was calculated from previous study and cultured on MacConkey and Trypton soy agar (TSA), as well as dignostics using 16S rRNA gene and result showed that all isolate was rturned to C. sakazakii and its included (6 from blood isolate (B), 6 from cerebrospinal fluid isolate (CSF),3 from Dialak milk (A),1 from NovolacAllernova \u00a0milk, (C1)1 from Novolac AD milk \u00a0(C2)).<\/span><\/h3>\n

This study todetectesome virulence factor of C. sakazakiiThe result\u00a0 showedthat all isolate could motility and swimming \u00a0on diameter range (17-79)mm and \u00a0swarming on diameter rang (4-64)mm , and to produce protease on diameter range\u00a0 (8-30) mm, and forming biofilm by microtiter plate method<\/span><\/h3>\n

The study effect of ( Citral,Curcumin, Inulin,Resveratrol, Trans-cinnamaldhyde) compound on C. sakazakii (A1b, B4 , CSF4) isolate\u00a0 at (1,10,100) microgram concentration \u00a0the result showed that all compounds could to inhibiteisolate by uses high concentration, while Trans-cinnamaldhyde usedcoulde to inhibate isolate by high and low concentration.<\/span><\/h3>\n

And the study of effect (Citral,Curcumin, Inulin,Resveratrol, Trans-cinnamaldhyde) compound on swimming and swarming motility of C. sakazakii (A1b,B4,CSF4) isolate, the result showed that all compound could to inhibite\u00a0 isolate\u00a0 on different ratio depended on type of compound and concentration.<\/span><\/h3>\n

And was studied\u00a0 effect of (Citral, Curcumin, Inulin,Resveratrol, Trans-cinnamaldhyde) compound on other virulence factor such as protease production on \u00a0(A1b, B4, CSF4) isolate,\u00a0 and the result showed inhibition ratio of Trans-cinnamaldhyde\u00a0 at 100 microgram concentration on (A1b, B4,CSF4) isolatein ratio100 % while\u00a0 medium inhibition ratio \u00a0\u00a0byCitral and \u00a0Inulin \u00a0\u00a0compound , and \u00a0low inhibition ratio \u00a0\u00a0by Curcumin and Resveratrol \u00a0compound on (A1b, B4) isolate, and on CSF4 isolate showed the inhibition ratio (Citral,Curcumin, Inulin,Resveratrol) at 100 microgram concentration more than 50microgram .<\/span><\/h3>\n

And \u00a0\u00a0result showed effect of all compound on C. sakazakii (A1b, B4,CSF4) isolate bio film formation\u00a0 at low and high concentration. And was studied \u00a0effect of different environment factors such as \u00a0temperature \u00a0and PH .The different \u00a0temperature degrees showed effect on C. sakazakii (Blood, Cerebrospinal fluid, milk) isolate \u00a0by use spectrophotometer\u00a0 and result showed\u00a0 at 55 C\u00ba\u00a0 was effect\u00a0\u00a0 to reduce growth bacteria compared on control the range (0.02-0.21)while on 25 \u00baCthe range was (0.14-0.31)compared with control 37 C the range was(0.38-0.66).<\/span><\/h3>\n

While study effect of pH on C. sakazakii was studied isolates result showed reduce growth at PH: 4 compared with control media\u00a0 and it was range of (0.02-0.29) and \u00a0range was (0.05-0.67)at\u00a0\u00a0 pH : 9compared with control \u00a0the range was (0.29-1.42) at PH 7 .<\/span><\/h3>\n

Some of the virulence genes of C. sakazakii were diagnosed \u00a0\u00a0such as zpxgene which incodes\u00a0 protease enzyme and flg J which incodes flagella formation and the result the precence\u00a0 of these genes on 100% in CSF4 isolate.<\/span><\/h3>\n

This study effect of (Citral,Curcumin,Inulin, Resveratrol, Trans-cinnamaldhyde) in amount gene expression of flgJ gene\u00a0 and showed the result to\u00a0 found variation in flgJ gene expression \u00a0in C. sakazakii (CSF4) isolate result showed\u00a0 the curcuminreduced flgJ gene expression to 0.2 folding change compared with control, while (Citral,Inulin,Resveratrol,Trans-cinnamaldhyde) at SMIC caused to increased flgJ gene expression to (12.6,20.7,19.4,6.3) folding change\u00a0 respectively compared with control.<\/span><\/h3>\n

The result of effect (Citral,Curcumin, Inulin , Resveratrol, Trans-cinnamaldhyde) at SMIC on zpxgene expression was variation amount gene after C. sakazakii (CSF4) isolate \u00a0treatment \u00a0and caused to increase zpx gene expression to ( 21.3, 18.7, 29.9, 34.1, 10.9) folding change respectively compared with control.<\/span><\/h3>\n

And the study effect of Trans-cinnamaldhyde at SMIC on\u00a0 flgJ gene expression\u00a0 of swimming and swarming media , the result showed to found variation in flgJ gene expression after C. sakazakii (CSF4) treatment \u00a0it was caused to reduce flgJ gene expression on swarming isolate to 0.14 folding change compared with control , and result showed to increase swimming isolate to 13.65 folding change compared with control .<\/span><\/h3>\n

While effect Trans-cinnamaldhyde at SMIC on zpx gene expression on swarming and swimming media, the result showed to found variasion zpxgene expression afterC. sakazakii (CSF4) isolate treatment\u00a0 it was increased zpx gene expression on swarming isolate \u00a0to 3.0 \u00a0compared with control , while result showed\u00a0 effect of Trans-cinnamaldhyde\u00a0 at\u00a0 SMIC \u00a0\u00a0causes to increased zpxgene expression in swimming isolate\u00a0 \u00a0to 44.8 folding change compared with control.<\/span><\/h3>\n","protected":false},"excerpt":{"rendered":"

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