{"id":28537,"date":"2019-12-02T14:43:22","date_gmt":"2019-12-02T11:43:22","guid":{"rendered":"http:\/\/ihcoedu.uobaghdad.edu.iq\/?p=28537"},"modified":"2020-02-13T12:37:30","modified_gmt":"2020-02-13T09:37:30","slug":"%d9%82%d8%b3%d9%85-%d8%b9%d9%84%d9%88%d9%85-%d8%a7%d9%84%d8%ad%d9%8a%d8%a7%d8%a9-%d9%8a%d9%86%d8%a7%d9%82%d8%b4-%d8%a7%d8%b7%d8%b1%d9%88%d8%ad%d8%a9-%d8%af%d9%83%d8%aa%d9%88%d8%b1%d8%a7%d9%87-%d8%aa-3","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=28537","title":{"rendered":"\u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u064a\u0646\u0627\u0642\u0634 \u0627\u0637\u0631\u0648\u062d\u0629 \u062f\u0643\u062a\u0648\u0631\u0627\u0647 \u062a\u0628\u062d\u062b \u0641\u064a \u062a\u0648\u0635\u064a\u0641 \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0627\u0644\u0643\u0628\u062f\u064a\u0629 (HCAM)"},"content":{"rendered":"

\u0646\u0627\u0642\u0634 \u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u062a\u0631\u0628\u064a\u0629 \u0644\u0644\u0639\u0644\u0648\u0645 \u0627\u0644\u0635\u0631\u0641\u0629 (\u0627\u0628\u0646 \u0627\u0644\u0647\u064a\u062b\u0645) \u0627\u0637\u0631\u0648\u062d\u0629 \u0627\u0644\u062f\u0643\u062a\u0648\u0631\u0627\u0647 \u0627\u0644\u0645\u0648\u0633\u0648\u0645\u0629 (\u062a\u0648\u0635\u064a\u0641 \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0627\u0644\u0643\u0628\u062f\u064a\u0629 ((HCAM \u0648\u0627\u0644\u0645\u0639\u0632\u0648\u0644\u0629 \u0645\u062d\u0644\u064a\u0627\u064b \u0645\u0646 \u0627\u0648\u0631\u0627\u0645 \u0627\u0644\u062c\u0647\u0627\u0632 \u0627\u0644\u0647\u0636\u0645\u064a \u0644\u0644\u0641\u0626\u0631\u0627\u0646 ) \u0644\u0644\u0637\u0627\u0644\u0628 ( \u0627\u064a\u0645\u0646 \u0631\u0627\u0636\u064a \u062d\u0628\u064a\u0628 \u0631\u0627\u0636\u064a ) \u0627\u0644\u062a\u064a \u0627\u0646\u062c\u0632\u0647\u0627 \u062a\u062d\u062a \u0627\u0634\u0631\u0627\u0641 \u0627\u0644\u062a\u062f\u0631\u064a\u0633\u064a\u0629 \u0641\u064a \u0627\u0644\u0642\u0633\u0645 (\u0623. \u0645. \u062f. \u0628\u064a\u062f\u0627\u0621 \u062d\u0633\u064a\u0646 \u0645\u0637\u0644\u0643 ) \u0648 (\u0623. \u0645. \u062f.\u00a0 \u0627\u062d\u0645\u062f \u0645\u062c\u064a\u062f \u0627\u0644\u0634\u0645\u0631\u064a ).<\/span><\/h4>\n

\"\"<\/p>\n

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\u0627. \u0645. \u062f \u0628\u064a\u062f\u0627\u0621 \u062d\u0633\u064a\u0646 \u0645\u0637\u0644\u0643\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0639\u0636\u0648\u0627 \u0648 \u0645\u0634\u0631\u0641\u0627<\/span><\/h4>\n

\u0627. \u0645. \u062f \u0627\u062d\u0645\u062f \u0645\u062c\u064a\u062f \u0627\u0644\u0634\u0645\u0631\u064a \u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0639\u0636\u0648\u0627 \u0648 \u0645\u0634\u0631\u0641\u0627<\/span><\/h4>\n

\"\"<\/p>\n

\u0648\u062a\u0647\u062f\u0641 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0649 \u062f\u0631\u0627\u0633\u0629 \u062e\u0635\u0627\u0626\u0635 \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a \u0644\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u0643\u0628\u062f \u0648\u0627\u0644\u0645\u0639\u0632\u0648\u0644 \u0645\u062d\u0644\u064a\u0627\u064b \u0645\u0646 \u0627\u0648\u0631\u0627\u0645 \u0627\u0644\u062c\u0647\u0627\u0632 \u0627\u0644\u0647\u0636\u0645\u064a.<\/span><\/h4>\n

\u0648\u0627\u0634\u0627\u0631 \u0627\u0644\u0637\u0627\u0644\u0628 \u0627\u0644\u0628\u0627\u062d\u062b \u0627\u0644\u0649 \u0627\u0646 \u062f\u0631\u0627\u0633\u0629 \u062e\u0635\u0627\u0626\u0635 \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a \u0644\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u0643\u0628\u062f \u0641\u064a \u0627\u0644\u0641\u0626\u0631\u0627\u0646 Hepatic Cancer Ahmed Majeed \u00a0\u00a0\u00a0(HCAM) \u062a\u0639\u062f \u0648\u0633\u064a\u0644\u0629 \u0645\u0647\u0645\u0629 \u0641\u064a \u0623\u0628\u062d\u0627\u062b \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u0643\u0628\u062f. \u0644\u0642\u062f \u0623\u0646\u0634\u0626 \u0647\u0630\u0627 \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a \u0645\u0646 \u0648\u0631\u0645 \u0627\u0635\u0627\u0628 \u0643\u0628\u062f \u0627\u0644\u0641\u0626\u0631\u0627\u0646 \u0627\u0644\u0628\u064a\u0636 \u0627\u0644\u0633\u0648\u064a\u0633\u0631\u064a\u0629\u00a0 Mus Musculus. \u0644\u0642\u062f \u0647\u062f\u0641\u062a \u0627\u0644\u062a\u062c\u0627\u0631\u0628 \u0627\u0644\u0645\u062e\u062a\u0644\u0641\u0629 \u0644\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u0627\u0644\u0649 \u0648\u0635\u0641 \u062e\u0637 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0627\u0644\u0643\u0628\u062f\u064a\u0629 HCAM \u060c \u0648\u0627\u0644\u062a\u064a \u062a\u0634\u0645\u0644 \u0627\u0644\u0627\u062a\u064a:<\/span><\/h4>\n

\u00a0\u00a0\u00a0\u00a0\u00a0 \u062a\u0646\u0645\u064a\u0629 \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a \u0644\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u0643\u0628\u062f \u0641\u064a \u0627\u062d\u062f\u0649 \u0627\u0644\u0627\u0648\u0633\u0627\u0637 \u0627\u0644\u0632\u0631\u0639\u064a\u0629 RPMI-1640 \u00a0\u0648\u062a\u0645\u062a \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u0632\u0631\u0639 \u0627\u0644\u062b\u0627\u0646\u0648\u064a \u0648\u0627\u062c\u0631\u0627\u0621 \u0627\u0644\u062a\u0645\u0631\u064a\u0631\u0627\u062a \u0627\u0644\u0644\u0627\u0632\u0645\u0629 \u0643\u0644 48- 72 \u0633\u0627\u0639\u0629. \u062a\u0645\u062a \u062f\u0631\u0627\u0633\u0629 \u0645\u0646\u062d\u0646\u0649 \u0627\u0644\u0646\u0645\u0648 \u0648\u0644\u062a\u0645\u0631\u064a\u0631\u0627\u062a \u0645\u062e\u062a\u0644\u0641\u0629 (5 -9 \u00a0– 12)\u00a0 \u0648\u0644\u0627\u0639\u062f\u0627\u062f \u0627\u0644\u062e\u0644\u0627\u064a\u0627 (10000 \u062e\u0644\u064a\u0629 \/ \u0645\u0644) \u0627\u0630 \u0643\u0627\u0646 \u0637\u0648\u0631 \u0627\u0644\u0633\u0643\u0648\u0646 lag phase \u00a0\u0641\u064a 24 \u0633\u0627\u0639\u0629 \u0628\u064a\u0646\u0645\u0627 \u0627\u0644\u0637\u0648\u0631 \u0627\u0644\u0644\u0648\u063a\u0627\u0631\u062a\u0645\u064a Log phase \u00a0\u0628\u064a\u0646 72 – 144 \u0633\u0627\u0639\u0629 \u0648\u0648\u0635\u0644\u062a \u0627\u0639\u062f\u0627\u062f \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0649 \u0637\u0648\u0631 \u0627\u0644\u0627\u0646\u062d\u062f\u0627\u0631 \u062a\u062f\u0631\u064a\u062c\u064a\u0623 \u0645\u0646 168 \u00a0– 240 \u0633\u0627\u0639\u0629 \u0641\u0636\u0644\u0623 \u0639\u0646 \u062d\u0633\u0627\u0628 \u0632\u0645\u0646 \u062a\u0636\u0627\u0639\u0641 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 Population Doubling Time (PDT) \u0627\u0630 \u0643\u0627\u0646 17 \u0633\u0627\u0639\u0629.<\/span><\/h4>\n

\u00a0\u00a0\u00a0\u00a0\u00a0 \u0627\u0638\u0647\u0631\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0648\u0631\u0627\u062b\u064a\u0629 \u0627\u0644\u062e\u0644\u0648\u064a\u0629 \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0648\u0644\u0644\u062a\u0645\u0631\u064a\u0631\u0627\u062a \u0627\u0644\u0627\u062a\u064a\u0629 (7- 8-12 )\u00a0 \u062a\u0634\u0648\u0647\u0627\u062a \u0643\u0631\u0648\u0645\u0648\u0633\u0648\u0645\u064a\u0629 \u0648\u062a\u063a\u064a\u0631\u0627\u062a \u0639\u062f\u062f\u064a\u0629 \u0641\u064a\u0645\u0627 \u0628\u064a\u0646\u0647\u0627, \u0648\u0645\u0646 \u0647\u0630\u0647 \u0627\u0644\u062a\u063a\u064a\u0631\u0627\u062a \u0627\u0644\u062a\u0631\u0643\u064a\u0628\u064a\u0629 \u0647\u064a :\u0627\u0644\u0637\u0648\u0644 \u0627\u0644\u063a\u064a\u0631 \u0637\u0628\u064a\u0639\u064a , \u062a\u0643\u0633\u0631 \u0627\u0630\u0631\u0639 \u0627\u0644\u0643\u0631\u0648\u0645\u0648\u0633\u0648\u0645\u0627\u062a \u0648\u063a\u064a\u0631\u0647\u0627.<\/span><\/h4>\n

\u00a0\u00a0\u00a0\u00a0\u00a0 \u0627\u0638\u0647\u0631\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0645\u0638\u0647\u0631\u064a\u0629 \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0648\u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0627\u0644\u0645\u062c\u0647\u0631 \u0645\u0642\u0644\u0648\u0628 \u0627\u0644\u0639\u062f\u0633\u0629 Inverted microscope \u0648\u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0645\u0644\u0648\u0646 \u0627\u0644\u0647\u064a\u0645\u0627\u062a\u0648\u0643\u0633\u0644\u064a\u0646 \u2013 \u0627\u064a\u0648\u0633\u064a\u0646 \u0648 \u0627\u0644\u0635\u0628\u063a\u0629 \u0627\u0644\u0628\u0646\u0641\u0633\u062c\u064a\u0629 \u0627\u0644\u0628\u0644\u0648\u0631\u064a\u0629 crystal violet \u00a0\u0648\u0644\u0644\u062a\u0645\u0631\u064a\u0631\u0627\u062a (6 , \u00a09 , 14) \u0627\u0646 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u062a\u0643\u0648\u0646 \u0628\u0634\u0643\u0644 \u0645\u062a\u0639\u062f\u062f \u0627\u0644\u0627\u0636\u0644\u0627\u0639 \u0645\u0634\u0627\u0628\u0647\u0629 \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0638\u0647\u0627\u0631\u064a\u0629 \u0641\u064a \u0627\u0644\u0646\u0645\u0648 \u062f\u0627\u062e\u0644 \u0642\u0627\u0631\u0648\u0631\u0629 \u0627\u0644\u0632\u0631\u0639 \u0627\u0644\u0646\u0633\u062c\u064a, \u0643\u0630\u0644\u0643 \u0627\u0638\u0647\u0631 \u0627\u0644\u0641\u062d\u0635 \u0627\u0644\u0645\u062c\u0647\u0631\u064a \u0627\u0646 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u062a\u0645\u062a\u0644\u0643 \u0646\u0648\u0627\u0629 \u0645\u0631\u0643\u0632\u064a\u0629 \u0648\u0646\u0648\u064a\u0627\u062a \u0645\u062a\u0639\u062f\u062f\u0629 \u0641\u0636\u0644\u0627 \u0639\u0646 \u0646\u0633\u0628\u0629 \u0627\u0644\u0633\u0627\u064a\u062a\u0648\u0628\u0644\u0627\u0632\u0645 \u0627\u0644\u0649 \u0627\u0644\u0646\u0648\u0627\u0629.<\/span><\/h4>\n

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0645\u0646 \u0646\u0627\u062d\u064a\u0629 \u0627\u062e\u0631\u0649 \u0627\u0638\u0647\u0631\u062a \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0621 \u0627\u0644\u062e\u0644\u0648\u064a\u0629 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 ICC))Immunocytochemistry \u00a0\u0648\u0644\u0644\u062a\u0645\u0631\u064a\u0631\u0627\u062a \u0627\u0644\u0627\u062a\u064a\u0629\u00a0 (17, 15 , 13) \u0641\u064a \u0634\u0631\u0627\u0626\u062d \u0627\u0644\u0632\u0631\u0639 \u0627\u0644\u0646\u0633\u062c\u064a \u0627\u0644\u0643\u0634\u0641 \u0639\u0646 \u0628\u0639\u0636 \u0627\u0644\u0627\u0646\u0632\u064a\u0645\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629. \u0645\u0646\u0647\u0627 : \u0627\u0644\u0627\u0646\u0632\u064a\u0645 P53 \u00a0\u0648\u0627\u0644\u0630\u064a \u0627\u0639\u0637\u0649 \u0646\u062a\u064a\u062c\u0629 \u0627\u064a\u062c\u0627\u0628\u064a\u0629 \u0648\u0627\u0636\u062d\u0629 ,\u0627\u0644\u0627\u0646\u0632\u064a\u0645 Epidermal growth factor receptor (EGFR) \u00a0\u0627\u0639\u0637\u0649 \u0646\u062a\u064a\u062c\u0629 \u0627\u064a\u062c\u0627\u0628\u064a\u0629 , \u0627\u0645\u0627 \u0627\u0644\u0627\u0646\u0632\u064a\u0645 (Human epidermal growth factor receptor (HER2\/neu \u0627\u0639\u0637\u0649 \u0646\u062a\u064a\u062c\u0629 \u0627\u064a\u062c\u0627\u0628\u064a\u0629 \u0636\u0639\u064a\u0641\u0629.<\/span><\/h4>\n

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u062a\u0645 \u062a\u0639\u0631\u064a\u0636 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0644\u0644\u0643\u0628\u062f \u0648\u0644\u0644\u062a\u0645\u0631\u064a\u0631\u0627\u062a \u0627\u0644\u0627\u062a\u064a\u0629 (16 , 19 , 21) \u0627\u0644\u0649 \u0646\u0648\u0639\u064a\u0646 \u0645\u0646 \u0627\u0644\u0639\u0644\u0627\u062c\u0627\u062a \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0626\u064a\u0629 ( \u0627\u0644\u0633\u064a\u0633\u0628\u0644\u0627\u062a\u064a\u0646 50 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/ \u0645\u0644 , \u0627\u0644\u062f\u0648\u0633\u064a\u062a\u0627\u0643\u0633\u0644 100 \u0645\u0627\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645 \/ \u0645\u0644 ) \u0648\u0644\u0645\u062f\u0629 72 \u00a0\u0633\u0627\u0639\u0629 \u0648\u0642\u062f \u0627\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0646 \u0627\u0644\u0633\u0645\u064a\u0629 \u0627\u0644\u062e\u0644\u0648\u064a\u0629 \u0644\u062e\u0644\u0627\u064a\u0627 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u0643\u0628\u062f \u0643\u0627\u0646\u062a \u0627\u0643\u062b\u0631 \u062d\u0633\u0627\u0633\u064a\u0629 \u0644\u0644\u0645\u0627\u062f\u062a\u064a\u0646 \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0626\u064a\u0629 \u0628\u0639\u062f \u0630\u0644\u0643 \u062a\u0645 \u062a\u0639\u0631\u064a\u0636 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062c\u0646\u064a\u0646\u064a\u0629 \u0627\u0644\u0644\u064a\u0641\u064a\u0629 \u0644\u0644\u0641\u0626\u0631\u0627\u0646 \u0644\u0644\u0645\u0627\u062f\u062a\u064a\u0646 \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0626\u064a\u0629 \u0648\u0644\u0646\u0641\u0633 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0648\u0627\u0644\u0645\u062f\u0629 \u0648\u0642\u062f \u0628\u064a\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0643\u0648\u0646 \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a \u0644\u0644 Mouse embryonic fibroblast (MEF) \u0643\u0627\u0646 \u0627\u0643\u062b\u0631 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u0645\u0627\u062f\u062a\u064a\u0646 \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0626\u064a\u0629.<\/span><\/h4>\n

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0627\u0638\u0647\u0631\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0628\u0639\u0636 \u0627\u0644\u062a\u063a\u064a\u0631\u0627\u062a \u0627\u0644\u0645\u0638\u0647\u0631\u064a\u0629 \u0639\u0646 \u0637\u0631\u064a\u0642 \u062a\u0635\u0628\u064a\u063a\u0647\u0627 \u0628\u0645\u0644\u0648\u0646 \u0627\u0644\u0647\u064a\u0645\u0627\u062a\u0648\u0643\u0633\u0644\u064a\u0646 \u0627\u064a\u0648\u0633\u064a\u0646 \u0641\u0642\u062f \u0644\u0648\u062d\u0638\u062a \u0628\u0639\u0636 \u0627\u0644\u062a\u063a\u064a\u0631\u0627\u062a \u0627\u0644\u0645\u0638\u0647\u0631\u064a\u0629 \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0627\u0644\u0643\u0628\u062f\u064a\u0629 \u0628\u0639\u062f \u062a\u0639\u0631\u0636\u0647\u0627 \u0644\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0626\u064a\u0629 \u0648\u0628\u062a\u0631\u0643\u064a\u0632 IC50\u00a0 (\u0627\u0644\u0633\u064a\u0633\u0628\u0644\u0627\u062a\u064a\u064610.74 \u00b5g\/ ml \u00a0\u0648\u0627\u0644\u062f\u0648\u0633\u064a\u062a\u0627\u0643\u0633\u064412.82 \u00b5g\/ ml\u00a0 \u00a0) \u0648\u0644\u0645\u062f\u0629 72 \u00a0\u0633\u0627\u0639\u0629. \u0648\u0645\u0646 \u0647\u0630\u0647 \u0627\u0644\u062a\u063a\u064a\u0631\u0627\u062a \u0647\u0648 \u062a\u062d\u0644\u0644 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062e\u0644\u0648\u064a \u0644\u0644\u062e\u0644\u0627\u064a\u0627 ,\u0627\u0646\u0643\u0645\u0627\u0634 \u0627\u0644\u0646\u0648\u0627\u0629 \u0648\u0636\u0645\u0648\u0631 \u0628\u0639\u0636 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0648\u0627\u0644\u0634\u0643\u0644 \u063a\u064a\u0631 \u0627\u0644\u0645\u0646\u062a\u0638\u0645 \u0644\u0628\u0639\u0636 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0648\u062a\u0641\u062c\u064a \u0627\u0644\u0633\u0627\u064a\u062a\u0648\u0628\u0644\u0627\u0632\u0645 \u0641\u0636\u0644\u0627 \u0639\u0646 \u0648\u062c\u0648\u062f \u0641\u0633\u062d \u0643\u0628\u064a\u0631\u0629 \u0628\u064a\u0646 \u0627\u0644\u062e\u0644\u0627\u064a\u0627.<\/span><\/h4>\n

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0648\u0628\u064a\u0646\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0648\u0631\u0627\u062b\u064a\u0629 \u0627\u0644\u062c\u0632\u064a\u0626\u064a\u0629 \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0627\u0644\u0645\u0632\u0631\u0648\u0639\u0629 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a \u0646\u062a\u064a\u062c\u0629 \u0627\u064a\u062c\u0627\u0628\u064a\u0629 \u0644\u0644\u0645\u0648\u0631\u062b \u00a0p53 \u0648\u03b2-actin \u00a0\u0648\u0633\u0644\u0628\u064a\u0629 \u0644\u0644\u0645\u0648\u0631\u062b \u0627\u0644\u0643\u0644\u0648\u0628\u064a\u0646\u03b2-globin .<\/span><\/h4>\n

\u00a0\u00a0\u00a0\u00a0\u00a0 \u0643\u0630\u0644\u0643 \u0628\u064a\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0644\u0645\u0648\u0631\u062b \u00a0p53 \u0641\u064a \u062e\u0644\u0627\u064a\u0627 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u0643\u0628\u062f \u0644\u0644\u0641\u0626\u0631\u0627\u0646 \u0627\u0644\u0628\u064a\u0636 \u0648\u0645\u0646 \u062e\u0644\u0627\u0644 \u0627\u0644\u0645\u0631\u0643\u0632 \u0627\u0644\u0648\u0637\u0646\u064a \u0644\u0645\u0639\u0644\u0648\u0645\u0627\u062a \u0627\u0644\u062a\u0642\u0627\u0646\u0629 \u0627\u0644\u0627\u062d\u064a\u0627\u0626\u064a\u0629 NCBI \u00a0\u0627\u0646 \u0647\u0646\u0627\u0644\u0643 \u062a\u0637\u0627\u0628\u0642 \u0628\u0646\u0633\u0628\u0629 94-99 % \u0628\u064a\u0646 \u0627\u062b\u0646\u064a\u0646 \u0645\u0646 \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0644\u0645\u0648\u0631\u062b .p53\u00a0<\/span><\/h4>\n

<\/h4>\n

\u0627\u0644\u062a\u0648\u0635\u064a\u0627\u062a<\/span><\/h4>\n

1- \u0627\u0646\u0634\u0627\u0621 \u062e\u0637 \u062e\u0644\u0648\u064a \u0644\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u0643\u0628\u062f \u0639\u0646 \u0627\u0644\u0627\u0646\u0633\u0627\u0646 \u0627\u0648 \u062e\u0637\u0648\u0637 \u062e\u0644\u0648\u064a\u0629 \u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0644\u0627\u062d\u062f \u0627\u0639\u0636\u0627\u0621 \u0627\u0644\u062c\u0647\u0627\u0632 \u0627\u0644\u0647\u0636\u0645\u064a \u0644\u0644\u0645\u0631\u0636\u0649 \u0627\u0644\u0639\u0631\u0627\u0642\u064a\u064a\u0646.<\/span><\/h4>\n

2- \u062f\u0631\u0627\u0633\u0629 \u062a\u0634\u062e\u064a\u0635\u064a\u0629 \u0644\u062e\u0637 \u062e\u0644\u0648\u064a \u0633\u0631\u0637\u0627\u0646\u064a \u062c\u062f\u064a\u062f \u0645\u062b\u0644 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u0645\u0631\u0626 , \u0627\u0644\u0645\u0639\u062f\u0629 \u0648\u0627\u0644\u0642\u0648\u0644\u0648\u0646 \u0645\u0646 \u062e\u0644\u0627\u0644 \u0627\u062c\u0631\u0627\u0621 \u062f\u0631\u0627\u0633\u0629 \u0648\u0631\u0627\u062b\u0629 \u062e\u0644\u0648\u064a\u0629 \u0648\u0645\u0646\u0627\u0639\u064a\u0629 \u0641\u0636\u0644\u0627\u064b \u0639\u0646 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0648\u0631\u0627\u062b\u064a\u0629 \u0627\u0644\u062c\u0632\u064a\u0626\u064a\u0629.<\/span><\/h4>\n

3- \u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0646\u064a\u0627\u062a \u062c\u062f\u064a\u062f\u0629 \u0644\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0648\u0631\u0627\u062b\u064a\u0629 \u0627\u0644\u062e\u0644\u0648\u064a\u0629 \u0645\u062b\u0644 FISH, SKY, GCH .<\/span><\/h4>\n

4- \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0628\u0639\u0636 \u0627\u0646\u0648\u0627\u0639 \u0627\u0644\u062c\u064a\u0646\u0627\u062a \u0628\u0648\u0633\u0627\u0637\u0629 \u062a\u0642\u0646\u064a\u0629 RT-PCR \u0628\u0639\u062f \u0645\u0639\u0627\u0645\u0644\u0629 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0628\u0627\u0644\u0639\u0644\u0627\u062c \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0626\u064a.<\/span><\/h4>\n

5- \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0633\u0645\u064a\u0629 \u0627\u0644\u062e\u0644\u0648\u064a\u0629 \u0644\u0627\u0646\u0648\u0627\u0639 \u0627\u062e\u0631\u0649 \u0644\u0644\u062e\u0637\u0648\u0637 \u0627\u0644\u062e\u0644\u0648\u064a\u0629 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629.\u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/h4>\n
\n

Characteristics study of cancer cell line isolated locally from digestive system tumors<\/span><\/h3>\n

By Ayman Radhi Habeeb<\/span><\/h3>\n

Supervised by Asst.prof.Baida’a H.Mutlak and Ahmed Majeed Al-Shumari<\/span><\/h3>\n

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 The characterization study of murineHepatic Cancer Ahmed Majeed(HCAM) is considers as a useful tool in research of liver cancer. This cell line was established from tumor of a white Swiss albino mouseMusMusculus. The different experiments of present study targeted to characterize HCAM cancer cell line, which are:<\/span><\/h3>\n

The continuous subculture characterization of the grown cell cultured in tissue culture flask each 48- 72 hours. studying the growth kinetics for different cells number of HCAM (10,000 cells \/1ml) at several passages 5, 9 and 12, and calculating population doubling time that have been 17 hours .The lag phase was identical for the curves 24hr, while the ranging of log phase from 72- 144 h, where the decline phase beginning at 168 h<\/span><\/h3>\n

The cytogenetic results of HCAM cell with passages 7, 8 and 12 revealed chromosomal variation with many numerical changes among the cancer cells along with structural changes including telocentric, abnormal length, and breaks in the arm of chromosomes<\/span><\/h3>\n

\u00a0The morphological study was carried out by using crystal violet, and hematoxylin&eosin with passages (6, 9 and 14). The cells were elongated multi-polar epithelial- like with central nucleus, multi-nuclei and clear vacuoles in cytoplasm<\/span><\/h3>\n

Epidermal growth factor receptor (EGFR), HER2-neu and P53 protein were detected using immunocyto chemistry in HCAM tumor cells at 13, 15 and 17 passage and the results were positive compared to negative control\u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/span><\/h3>\n

HCAM cells at several passages 16, 19 and 21 were exposed to chemotherapy agents with serial dilutions, as the following Cisplatin 50, 25, 12.5, 6.25 and 3.125 \u00b5g\/ml and Docetaxel 100, 50, 25, 12.5, 6.25 \u00b5g\/ml for 72 h to determine the inhibition rate of cancer cells and compared with MEF cell line after exposure to Cisplatin and Docetaxel at the same concentrations and duration.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/h3>\n

The inhibition rate measured all treated and untreated cells which were 65.4, 60.1, 56.4, 52.6 and 47.0 % by Cisplatin and 59.6, 57.2, 51.4, 42.9 and 37.5% by Docetaxel.All these concentrations of chemotherapy agent were had inhibitory effect on HCAM cells and the results revealed that HCAM cell were sensitive to Cisplatin and Docetaxel, While MEF cell after being exposed to Cisplatin and Docetaxel, showed resistance to chemotherapy compared with HCAM cells<\/span><\/h3>\n

Analysis of changes in cell morphology was evaluated using hematoxylin and eosin staining .Many morphological changes were characterized on HCAM cancer cells after exposure to IC50 concentration of chemotherapy (Cisplatin&Doetaxel), the morphological changes represented by the volume and shape of the nucleus\/cytoplasm ratio ,degeneration, and Irregular shape of cells and shrink of nucleus<\/span><\/h3>\n

In addition, the present study conducted the PCR and DNA sequencing of some genes, The P53 gene product exhibited specific band at 500 bp and \u03b2-actin gene product exhibited band at 400 bp on gel electrophoresis whereas, the \u03b2-globin gene product showed no specific band on the gel electrophoresis. Based on P53 gene product partial sequencing NCBI blast result, it showed similarity with two partials sequenced P53<\/span><\/h3>\n

Establishment of human hepatocellular carcinoma cell line or other digestive tumor cell lines from Iraqi patients<\/span><\/h3>\n

– Characterization study for new digestive cancer cell lines such as esophagus, stomach and colon cell lines by the detection of ICC, cytogenetic, cytotoxicity and molecular studies<\/span><\/h3>\n

– Using other techniques to observe the chromosomal alterations\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 such as Spectral Karyotyping(SKY),Fluorescence in situ hybridization(FISH) and Comparative Genomic hybridization(GCH).<\/span><\/h3>\n

– Measure the level of some genes expression in cancer cell after treatment with chemotherapy agents by RT-PCR.<\/span><\/h3>\n

– Conducting further cytotoxic in-vitro studies on the other digestive\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 tumor cell lines.<\/span><\/h3>\n

–\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Studying the angiogenesis process in-vivo by initiating tumors by implantation of (HCAM) cells in the laboratory animals, then examin the effects of angiogenesis inhibiter by tested many anti-angiogenesis therapies according to tumor size and distance metastasis in laboratory animals.<\/span><\/h3>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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