{"id":28643,"date":"2019-12-10T21:23:46","date_gmt":"2019-12-10T18:23:46","guid":{"rendered":"http:\/\/ihcoedu.uobaghdad.edu.iq\/?p=28643"},"modified":"2020-02-13T12:36:38","modified_gmt":"2020-02-13T09:36:38","slug":"28643","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=28643","title":{"rendered":"\u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u064a\u0646\u0627\u0642\u0634 \u0627\u0637\u0631\u0648\u062d\u0629 \u062f\u0643\u062a\u0648\u0631\u0627\u0647 \u062a\u0628\u062d\u062b \u0641\u064a \u062f\u0648\u0631 \u0641\u064a\u062a\u0627\u0645\u064a\u0646 C \u0648\u0647\u0631\u0645\u0648\u0646 \u0627\u0644\u0628\u0631\u0627\u0633\u064a\u0646\u0648\u0644\u0627\u064a\u062f \u0641\u064a \u062a\u062d\u0645\u0644 \u0646\u0628\u0627\u062a \u0627\u0644\u0645\u0627\u0634 \u0644\u0644\u0627\u062c\u0647\u0627\u062f \u0627\u0644\u0645\u0644\u062d\u064a"},"content":{"rendered":"

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    • \n

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    • \n

      \u062a\u0641\u0648\u0642 \u0627\u0644\u0633\u0648\u064a\u0642\u062a\u064a\u0646 \u0627\u0644\u062c\u0646\u064a\u0646\u062a\u064a\u0646 \u0627\u0644\u0639\u0644\u064a\u0627(<\/strong>Epicotyle<\/strong>) \u0648\u0627\u0644\u0633\u0641\u0644\u0649(<\/strong>Hypocotyl<\/strong>) \u0641\u064a \u0627\u0639\u0637\u0627\u0621 \u0627\u0639\u0644\u0649 \u0646\u0633\u0628\u0629 \u0644\u0627\u0633\u062a\u062d\u062b\u0627\u062b \u0627\u0644\u0643\u0627\u0644\u0633 \u0639\u0646\u062f \u0627\u0644\u062a\u0648\u0644\u064a\u0641\u0629 \u0627\u0644\u0647\u0631\u0645\u0648\u0646\u064a\u0629 0.5\u0645\u0644\u063a\u0645.\u0644\u062a\u0631-1<\/sup> \u0645\u0646 <\/strong>NAA<\/strong> + 2.0\u0645\u0644\u063a\u0645.\u0644\u062a\u0631-1<\/sup> \u0645\u0646<\/strong>BA<\/strong>.<\/strong><\/span><\/h4>\n<\/li>\n
    • \n

      \u0627\u0646 \u0627\u0644\u0643\u0627\u0644\u0633 \u0627\u0644\u0645\u0632\u0631\u0648\u0639 \u0641\u064a \u0648\u0633\u0637 <\/strong>MS)<\/strong>) <\/strong>Murashige and Skoog<\/strong> \u0627\u0644\u0645\u0636\u0627\u0641 \u0627\u0644\u064a\u0647 \u062a\u0631\u0627\u0643\u064a\u0632 <\/strong>NaCl<\/strong>(6, 9, 12, 15) \u062f\u064a\u0633\u064a\u0633\u0645\u0646\u0632.\u0645-1<\/sup> \u0627\u0646\u062e\u0641\u0636 \u062a\u062f\u0631\u064a\u062c\u064a\u0627 \u0648\u0645\u0639\u0646\u0648\u064a\u0627 \u0641\u064a \u0645\u0624\u0634\u0631\u0627\u062a\u0647 \u0627\u0644\u0645\u062f\u0631\u0648\u0633\u0629 (\u0627\u0644\u0648\u0632\u0646\u064a\u0646 \u0627\u0644\u0637\u0631\u064a \u0648\u0627\u0644\u062c\u0627\u0641, \u062f\u0644\u064a\u0644 \u062a\u062d\u0645\u0644 \u0627\u0644\u0627\u062c\u0647\u0627\u062f \u0627\u0644\u0645\u0644\u062d\u064a, \u0645\u0639\u062f\u0644\u064a \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0645\u0637\u0644\u0642 \u0648\u0627\u0644\u0646\u0633\u0628\u064a, \u0627\u0633\u062a\u062f\u0627\u0645\u0629 \u0627\u0644\u0643\u062a\u0644\u0629 \u0627\u0644\u062d\u064a\u0648\u064a\u0629, \u0645\u062d\u062a\u0648\u0649 \u0627\u0644\u0639\u0646\u0627\u0635\u0631(<\/strong>N<\/strong>,<\/strong>P<\/strong>,<\/strong>K<\/strong>, <\/strong>Ca<\/strong>, <\/strong>Mg<\/strong>) \u0648\u0646\u0633\u0628\u0629 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646) \u0644\u0627\u0633\u064a\u0645\u0627 \u0641\u064a \u0627\u0644\u062a\u0631\u0643\u064a\u0632 15\u062f\u064a\u0633\u064a\u0633\u0645\u0646\u0632.\u0645-1 <\/sup>\u0648\u0628\u0646\u0633\u0628\u0629 (52.41, 55.91, 55.91, 79.84, 79.78, 38.51, 68.95, 54.21, 65.63, 57.21, 71.90 , 29.51)% \u0628\u0627\u0644\u062a\u062a\u0627\u0628\u0639, \u0648\u0632\u064a\u0627\u062f\u0629 \u0645\u0639\u0646\u0648\u064a\u0629 \u0641\u064a \u0627\u0644\u0646\u0636\u062d \u0627\u0644\u0627\u0644\u0643\u062a\u0631\u0648\u0644\u064a\u062a\u064a \u0627\u0644\u0646\u0633\u0628\u064a \u0648\u0645\u062d\u062a\u0648\u0649 \u0627\u0644\u0635\u0648\u062f\u064a\u0648\u0645 \u0648\u0627\u0644\u0643\u0644\u0648\u0631 \u0648\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0628\u0631\u0648\u0644\u064a\u0646 \u0648\u0628\u0646\u0633\u0628\u0629 (43.21, 41.48, 37.09, 333.67)% \u0628\u0627\u0644\u062a\u062a\u0627\u0628\u0639 \u0642\u064a\u0627\u0633\u0627 \u0628\u0627\u0644\u062a\u0631\u0643\u064a\u0632 6 \u062f\u064a\u0633\u064a\u0633\u0645\u0646\u0632.\u0645-1<\/sup>.<\/strong><\/span><\/h4>\n<\/li>\n
    • \n

      \u0644\u0648\u062d\u0638 \u0627\u0646 \u062a\u0631\u0627\u0643\u064a\u0632 \u0641\u064a\u062a\u0627\u0645\u064a\u0646<\/strong>C<\/strong> (0, 20, 40)\u0645\u0644\u063a\u0645.\u0644\u062a\u0631-1 <\/sup>\u0648\u0647\u0631\u0645\u0648\u0646 \u0627\u0644\u0628\u0631\u0627\u0633\u064a\u0646\u0648\u0644\u0627\u064a\u062f (0, 0.02, 0.04) \u0645\u0644\u063a\u0645.\u0644\u062a\u0631-1 <\/sup>\u0633\u0628\u0628\u062a \u0632\u064a\u0627\u062f\u0629 \u0641\u064a \u0645\u0624\u0634\u0631\u0627\u062a \u0627\u0644\u0643\u0627\u0644\u0633 \u0627\u0639\u0644\u0627\u0647 \u0644\u0627\u0633\u064a\u0645\u0627 \u0627\u0644\u062a\u0631\u0643\u064a\u0632\u064a\u0646 40\u0645\u0644\u063a\u0645.\u0644\u062a\u0631-1<\/sup> \u0648 0.04\u0645\u0644\u063a\u0645.\u0644\u062a\u0631-1<\/sup> \u0645\u0646 \u0627\u0644\u0641\u064a\u062a\u0627\u0645\u064a\u0646 \u0648\u0627\u0644\u0647\u0631\u0645\u0648\u0646 \u0628\u0627\u0644\u062a\u062a\u0627\u0628\u0639.<\/strong><\/span><\/h4>\n<\/li>\n
    • \n

      \u0644\u0648\u062d\u0638 \u0627\u0646 \u0627\u0644\u062a\u062f\u0627\u062e\u0644\u0627\u062a \u0627\u0644\u062b\u0646\u0627\u0626\u064a\u0629 \u0648\u0627\u0644\u062b\u0644\u0627\u062b\u064a\u0629 \u0628\u064a\u0646 \u0639\u0648\u0627\u0645\u0644 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062b\u0644\u0627\u062b \u0645\u0648\u062b\u0631\u0629 \u0628\u0635\u0648\u0631\u0629 \u0645\u0639\u0646\u0648\u064a\u0629, \u0648\u0627\u0639\u0637\u062a \u0627\u0644\u062a\u0648\u0644\u064a\u0641\u0629 \u0627\u0644\u0645\u0631\u0643\u0628\u0629 \u0645\u0646 40\u0645\u0644\u063a\u0645.\u0644\u062a\u0631-1 <\/sup>\u0641\u064a\u062a\u0627\u0645\u064a\u0646<\/strong>C<\/strong> \u06480.02\u0645\u0644\u063a\u0645.\u0644\u062a\u0631-1<\/sup> \u0628\u0631\u0627\u0633\u064a\u0646\u0648\u0644\u0627\u064a\u062f \u0627\u0641\u0636\u0644 \u0645\u0639\u0627\u0644\u062c\u0629 \u0644\u0627\u0636\u0631\u0627\u0631 \u0627\u0644\u0627\u062c\u0647\u0627\u062f \u0627\u0644\u0645\u0644\u062d\u064a \u0644\u0627\u0633\u064a\u0645\u0627 \u0641\u064a \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0645\u0644\u062d\u064a 15\u062f\u064a\u0633\u064a\u0633\u0645\u0646\u0632.\u0645-1<\/sup>. <\/strong><\/span><\/h4>\n<\/li>\n
    • \n

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    • \n

      \u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u0627\u062f\u0627\u0621 \u0627\u0644\u062d\u0642\u0644\u064a \u0644\u0644\u0646\u0628\u0627\u062a\u0627\u062a \u0627\u0644\u0646\u0627\u062a\u062c\u0629 \u0645\u0646 \u0627\u0644\u0643\u0627\u0644\u0633 \u0627\u0644\u0645\u0639\u0631\u0636 \u0644\u0627\u062c\u0647\u0627\u062f \u0627\u0644\u0645\u0644\u062d\u064a, \u0645\u0639 \u062a\u0641\u0648\u0642 \u0627\u0644\u0646\u0628\u0627\u062a\u0627\u062a \u0627\u0644\u0646\u0627\u062a\u062c\u0629 \u0645\u0646 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 6\u062f\u064a\u0633\u064a\u0633\u0645\u0646\u0632.\u0645-1<\/sup> \u0641\u064a \u0645\u0639\u0638\u0645 \u0627\u0644\u0635\u0641\u0627\u062a \u0627\u0644\u0645\u062f\u0631\u0648\u0633\u0629. <\/strong><\/span><\/h4>\n<\/li>\n<\/ul>\n

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        \n
      • \n

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      • \n

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      • \n

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      • \n

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        \u00a0<\/strong><\/span><\/h4>\n

        \u00a0<\/strong><\/span><\/h4>\n

        \u0648 \u0627\u0648\u0635\u0649 \u0627\u0644\u0637\u0627\u0644\u0628 \u0641\u064a \u062e\u062a\u0627\u0645 \u0627\u0637\u0631\u0648\u062d\u062a\u0647 \u0628\u0627\u0644\u062a\u0648\u0635\u064a\u0627\u062a \u0627\u0644\u0627\u062a\u064a\u0629 :<\/strong><\/span><\/h4>\n
          \n
        • \n

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        • \n

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        • \n

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        • \n

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        • \n

          \u0627\u062c\u0631\u0627\u0621 \u062f\u0631\u0627\u0633\u0627\u062a \u0645\u0643\u062b\u0641\u0629 \u0639\u0644\u0649 \u0627\u0644\u0645\u0648\u0627\u062f \u0627\u0644\u0641\u0639\u0627\u0644\u0629(\u0646\u0648\u0639\u064a\u0629 \u0648\u0643\u0645\u064a\u0629) \u0644\u0646\u0628\u0627\u062a \u0627\u0644\u0645\u0627\u0634 \u0648\u0627\u062f\u0631\u0627\u062c\u0647 \u0636\u0645\u0646 \u0627\u0644\u0646\u0628\u0627\u062a\u0627\u062a \u0627\u0644\u0637\u0628\u064a\u0629 \u0641\u064a \u0627\u0644\u0643\u062a\u0628 \u0648\u0627\u0644\u0628\u062d\u0648\u062b.<\/strong><\/span><\/h3>\n
          \n

          Role of Vitamin C and Brassinolide Hormone in Tolerance of Mung bean plant (Vigna radiata L.) for Salt Stress in Tissue Culture and Field\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0<\/span><\/h3>\n

          Hassan Abdul Razaq Ali Hussein<\/span><\/h3>\n

          Supervised by Wifak A.M. Al-Kasiay and Ekhlas A.J. El-Kaaby\u00a0<\/span><\/h3>\n

          Abstract<\/span><\/h3>\n

          In vitro experiment was conducted at the Genetic Engineering Department, Directorate of Agricultural Research \/ Ministry of Science and Technology according to completely randomized design and In vivo experiment was carried out in the College of Education for pure sciences (Ibn al-Haitham) \/ University of Baghdad during the growing season 2018 according to randomized completely block design to study the effect the concentrations of sodium chloride(NaCl), vitamin C and brassinolide hormone on the mungbean plant (Vigna radiata L.).<\/span><\/h3>\n

          The tissue culture experiment included: seed sterilizing, selecting the appropriate plant part to induce the callus with different hormonal combinations, study some of the callus parameters and estimate the active compounds as well as regeneration, rooting and acclimatization.<\/span><\/h3>\n

          The results showed that surface sterilization of seeds was achieved using 70% ethanol for 30second followed by 15min with 2% NaOCl.<\/span><\/h3>\n

          \u00a0The best callus induction in epicotyle and hypocotyle within the hormonal combination 0.5mg.l-1 of NAA and 2.0mg.l-1 of BA.<\/span><\/h3>\n

          The\u00a0 results also showed that the callus cultured in MS media with NaCl concentrations (6, 9, 12 and 15)dS.m-1 was significantly reduced\u00a0 and gradated in studied parameters (fresh and dry weight, salt stress index, absolute and relative rates, biomass duration, elements content\u00a0 (N, P, K, Ca and Mg) and protein percentage especially at the level of 15dS.m-1 with (52.41, 55.91, 55.91, 79.84, 79.78, 38.51, 68.95, 54.21, 65.63, 57.21, 71.90 and 29.51)% respectively and a significant increase in relative electrolyte leakage, content of sodium\u00a0 and chloride and proline concentration with (43.21, 41.48, 37.09, 333.67)% respectively compared to the concentration of 6dS.m-1.<\/span><\/h3>\n

          \u00a0The vitamin C concentrations(0, 20, and 40)mg.l-1 and brassinolide hormone concentrations(0, 0.02, and 0.04)mg.l-1 caused an increase in the above callus parameters especially 40mg.l-1 and 0.04mg.l-1 concentrations of vitamin and hormone respectively. \u00a0<\/span><\/h3>\n

          The two and triple interactions were significant and the treatment (40mg.l-1 vitamin C and 0.02mg.l-1 hormone) was best to treat the stress (15dS.m-1).<\/span><\/h3>\n

          The regenerated shoots result from callus exhibition to salt concentrations (6, 9, and 12) dS.m-1 and cultured on MS medium containing three different types of hormonal combinations (0.2 TDZ, 1.01ZE5, BAP +20Ads)mg.l-1 and then were sub cultured on MS medium supplemented with IBA 1mg.l-1 for rooting. Regenerated plantlets and were acclimatized and grown in poly-house condition to compare with plants resulting from seed which were grown in the MS media. Morphology parameters (plant height, leaf area, branches No., dry weight and chlorophyll content) and yield contents (pods No., seed No. in pod and 100seed weight) were studied.<\/span><\/h3>\n

          Concerning to field performance evaluation of stressed callus derivative plants, the results revealed that plants produced from 6dS.m-1 were superior in most of parameters under studied.<\/span><\/h3>\n

          Moreover, using the Random Amplified Polymorphic-DNA(RAPD) molecular marker to detect the genetic variations of the plants resulting from callus induced after salt stress. The RAPD marker results showed, a genetic variation in regenerated plants which conformed by the number and size of bands.<\/span><\/h3>\n

          The field experiment included studying the use of salted water at the concentrations (5.5, 10 and 15)dS.m-1 as well as control treatment 0.9dS.m-1 (tap water) in addition to foliar application at three concentrations of vitamin C (0, 60, and 120)mg.l-1 and brassinolide (0, 2 and 4)mg.l-1 and their interaction on morphology and physiology parameters, flower growth, yield contents and active compound in seed.<\/span><\/h3>\n

          Results indicated that a gradual decrease and\u00a0 significant effect on studied parameters(plant height, leaf area, branches No., dry weight, chlorophyll content, carbohydrates percentage, inflorescences No., flower\u00a0 No., pod No., seed No. in pod, 100seed weight, seed yield and protein percentage) with increase of salt concentrations especially at 15dS.m-1 compare to the control treatment with (31.09, 23.88, 49.74, 33.61, 25.49, 38.82, 36.50, 35.20, 37.09, 23.28. 32.86, 29.40 and 28.16)% respectively, While a significant increase in enzymatic(catalase and peroxidase) and non enzymatic antioxidants(proline, salicylic acid, vitamin C and \u03b1-tocopherol) reached (124.22, 123.03, 83.01, 282.87, 29.04 and 13.10)% respectively.<\/span><\/h3>\n

          The foliar application with vitamin C and brassinolide especially the concentrations (120 and 4)mg.l-1 respectively affected significantly on the increase of all characteristic, enzymatic and non-enzymatic antioxidants which mentioned above.<\/span><\/h3>\n

          The two and triple interactions showed a significant effect on the studied parameters and antioxidants, with the superiority of plants irrigated with tap water and sprayed with 120mg.l-1 of\u00a0 vitamin C and 2mg.l-1 of brassinolide to other plants, while the lowest values in the plants irrigated with salt water (15dS.m-1) only. The foliar application of vitamin and hormone reduction of the negative effects of salt stress, especially in the upper concentrations of the above factors, with a synergy between vitamin and the hormone in most of the studied characteristic and antioxidants.<\/span><\/h3>\n

          High Performance Liquid Chromatography (H.P.L.C) was used to detect the presence of active compounds in callus and seeds of mungbean, the results revealed (15dS.m-1 + 40mg.l-1vitamin + 0.04 mg.l-1 hormone) and (15dS.m-1 + 120mg.l-1vitamin + 4mg.l-1hormone) treatments were superior in most of active compounds for callus and seed respectively.<\/span><\/h3>\n

           <\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"

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