{"id":28931,"date":"2020-01-05T09:41:22","date_gmt":"2020-01-05T06:41:22","guid":{"rendered":"http:\/\/ihcoedu.uobaghdad.edu.iq\/?p=28931"},"modified":"2020-02-13T12:33:18","modified_gmt":"2020-02-13T09:33:18","slug":"%d9%85%d9%86%d8%a7%d9%82%d8%b4%d8%a9-%d8%b1%d8%b3%d8%a7%d9%84%d8%a9-%d9%85%d8%a7%d8%ac%d8%b3%d8%aa%d9%8a%d8%b1-%d8%aa%d8%a8%d8%ad%d8%ab-%d9%81%d9%8a-%d8%a7%d8%b3%d8%aa%d8%b9%d9%85%d8%a7%d9%84-%d8%a7","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=28931","title":{"rendered":"\u0645\u0646\u0627\u0642\u0634\u0629 \u0631\u0633\u0627\u0644\u0629 \u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u062a\u0628\u062d\u062b \u0641\u064a \u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0627\u0644\u0641\u0637\u0631 Penicillium chrysogenum \u0644\u062a\u062e\u0644\u064a\u0642 \u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0627\u0644\u0641\u0636\u064a\u0629 \u0645\u062a\u0646\u0627\u0647\u064a\u0629 \u0627\u0644\u0635\u063a\u0631"},"content":{"rendered":"

<\/h4>\n

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\"\"<\/p>\n

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    \n
  1. \n

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    2-\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0628\u0646\u0627\u0621 \u0648\u062a\u0648\u0635\u064a\u0641 \u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0627\u0644\u0641\u0636\u064a\u0629 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0627\u0644\u0641\u0637\u0631Penicillium chrysogenum \u0648\u0628\u0623\u0646\u0648\u0627\u0639 \u0645\u062e\u062a\u0644\u0641\u0629 \u0645\u0646 \u0627\u0644\u0645\u062c\u0627\u0647\u0631.<\/span><\/h4>\n

    3-\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u062a\u062d\u062f\u064a\u062f \u0623\u0642\u0644 \u062a\u0631\u0643\u064a\u0632 \u0645\u062b\u0628\u0637\u00a0 (The Minimum Inhibitory Concentration (MIC \u0644\u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0627\u0644\u0641\u0636\u064a\u0629 \u0636\u062f \u062e\u0645\u064a\u0631\u0629 Candida glabrata .<\/span><\/h4>\n

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    5-\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u0623\u062b\u064a\u0631\u0627\u062a \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0627\u0644\u0641\u0636\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u062a\u063a\u064a\u0631\u0627\u062a \u0627\u0644\u0646\u0633\u064a\u062c\u064a\u0629 \u0644\u0644\u0641\u0626\u0631\u0627\u0646 \u0627\u0644\u0645\u0635\u0627\u0628\u0629 \u0628\u0627\u0644\u062e\u0645\u064a\u0631\u0629 Candida glabrata.<\/span><\/h4>\n

    \"\"<\/h4>\n

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    \n

    Synthesis, Characterization and Anti fungal activity of Silver Nano Particles Using Penicillium chrysogenum extract against Bio film formation in Candida glabrata<\/span><\/h3>\n

    By Nada Sadiq Salih<\/span><\/h3>\n

    Supervised by Asst.prof.Dr.Thamir A.A Muhsen<\/span><\/h3>\n

    Summary<\/span><\/h3>\n

    The distribution of patients with candidiasis and oral disease showed that the age group 50-65 years recorded the highest incidence of candidiasis in women and men with leukemia by 50% and 37.9 % respectively compared to the lowest incidence of candidiasis in the age group under 17 years in women and men 8.8% and 13.5%, respectively, while the age group between 5-8 years was high, reaching 18 cases of oral candidiasis and 42% of children with leukemia compared with 8-10 years, which was the least. It reached 9 cases (21%) and found very significant differences between the treatment of women and men,No significant differences were recorded among the children. The highest incidence of Candida glabrata was 59 isolates of women and men with leukemia, while C. kefyer was the least and only one isolate. C. glabrata was the most isolated from children with leukemia 13and C.kefyer was only 2.<\/span><\/h3>\n
      \n
    1. \n

      glabrata showed the highest bio film formation, 48 isolates were distributed between 21 strong and 27 weak biofilm production, The least biofilm producing isolates were C.tropicalis, which reached 6 isolates and distributed between 4strong and 2weak,There were very significant differences between the biofilm and different Candida spp. The color change was observed after the biomass of Peniclliumchrysogenum was mixed with the solution of silver nitrate from yellow to brown due to the presence of surface plasmons,\u00a0For particle characterization, an atomic force microscope was used and the mean square root value was 2.12 nm and the surface roughness rate of the membrane was 1.54 nm\u00a0this value is a guide to surface roughness, the highest peak with a wavelength of 455 nm has emerged due to the presence of surface plasmons and another peak with a wavelength of 243 nm these indicate the presence of Tyrosine and Tryptophan residues in the protein released from the fungus. when using UV spectrometer the results of the scanning electron microscope showed spherical granules of varying sizes some clusters are regularly distributed during crystalline growth and of nanoscale sizes.<\/span><\/h3>\n<\/li>\n<\/ol>\n

      The results of determining the minimum inhibitory concentration of silver nanoparticles were recorded that all concentrations used were effective in inhibiting C.glabrata, The best concentration was 20 mM, which recorded the highest inhibition zone of 13 mm, the lowest concentration was 5 mM, which showed the lowest inhibition zone at 7 mm and the differences were very significant.C. glabrata was observed for adhesion to epithelial cells in the absence of silver nanoparticles and antifungal whereas when silver nanoparticles were added, yeast lost its ability to adhere, the addition of the anti-fungal fluconazole caused reducing the yeast adhesion to the epithelial cells, when collecting silver nanoparticles and fluconzole, the C.glabrata is lost to final adhesion and caused her death.Histopathological results of mice liver showed that control group display normal liver lobules, liver sinusoids ,central vein and normal shape hepatocytes with no visible damage or histological changes.<\/span><\/h3>\n

      The group infected with Candida glabratahad obvious histological changes such as display dilated congested central vein , congestion portal vein, bi-nucleated cells are also seen and inflammatory cell infiltration.The results of a group that were given silver nano particles observed increasing amount of connective tissue fibers,chronic inflammatory cell infiltration, hepatocytes have darkly-stained nuclei,and vacuolated cytoplasm could be established. While the group of mice infected with the fungus Candida glabrata and treated with silver nanoparticles and fluconzole showed slightly dilated sinusoids, central vein with flat endothelial lining ,Mononuclear cellular infiltration,hepatocytes with vesicular nuclei and cytoplasm ,darkly-stained nuclei,less vacuolated cytoplasm and bi-nucleated cells\u00a0<\/span><\/h3>\n

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