{"id":32810,"date":"2020-08-15T18:05:52","date_gmt":"2020-08-15T15:05:52","guid":{"rendered":"http:\/\/ihcoedu.uobaghdad.edu.iq\/?p=32810"},"modified":"2020-09-20T12:55:16","modified_gmt":"2020-09-20T09:55:16","slug":"32810","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=32810","title":{"rendered":"\u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u064a\u0646\u0627\u0642\u0634 \u0631\u0633\u0627\u0644\u0629 \u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u062a\u0628\u062d\u062b \u0641\u064a \u062a\u0623\u062b\u064a\u0631 \u0628\u0639\u0636 \u0645\u062b\u0628\u0637\u0627\u062a \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0627 Proteus mirabilis \u0627\u0644\u0645\u0639\u0632\u0648\u0644\u0629 \u0645\u0646 \u0627\u0644\u062d\u0631\u0648\u0642 \u0648 \u0627\u0644\u062c\u0631\u0648\u062d"},"content":{"rendered":"

\"\"<\/h4>\n

\u0646\u0627\u0642\u0634 \u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u062a\u0631\u0628\u064a\u0629 \u0644\u0644\u0639\u0644\u0648\u0645 \u0627\u0644\u0635\u0631\u0641\u0629 (\u0627\u0628\u0646 \u0627\u0644\u0647\u064a\u062b\u0645) \u0631\u0633\u0627\u0644\u0629 \u0627\u0644\u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u0627\u0644\u0645\u0648\u0633\u0648\u0645\u0629 (\u062f\u0631\u0627\u0633\u0629 \u062a\u0623\u062b\u064a\u0631 \u0628\u0639\u0636 \u0645\u062b\u0628\u0637\u0627\u062a \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0627 Proteus mirabilis<\/em> \u0627\u0644\u0645\u0639\u0632\u0648\u0644\u0629 \u0645\u0646 \u0627\u0644\u062d\u0631\u0648\u0642 \u0648 \u0627\u0644\u062c\u0631\u0648\u062d ) \u0644\u0644\u0637\u0627\u0644\u0628 ( \u0623\u062d\u0645\u062f \u0648\u0633\u0627\u0645 \u0639\u0632\u064a\u0632 ) \u0627\u0644\u062a\u064a \u0627\u0646\u062c\u0632\u0647\u0627 \u062a\u062d\u062a \u0627\u0634\u0631\u0627\u0641 \u0627\u0644\u062a\u062f\u0631\u064a\u0633\u064a\u0629 \u0641\u064a \u0627\u0644\u0642\u0633\u0645 ( \u0623.\u0645.\u062f. \u00a0\u0633\u0639\u0627\u062f \u062e\u0644\u064a\u0644 \u0625\u0628\u0631\u0627\u0647\u064a\u0645 ) \u060c \u0648\u0646\u0648\u0642\u0634\u062a \u0645\u0646 \u0642\u0628\u0644 \u0644\u062c\u0646\u0629 \u0627\u0644\u0645\u0646\u0627\u0642\u0634\u0629 \u0627\u0644\u062a\u064a \u062a\u0623\u0644\u0641\u062a \u0645\u0646 \u0627\u0644\u0630\u0648\u0627\u062a \u0627\u0644\u0645\u062f\u0631\u062c\u0629 \u0627\u0633\u0645\u0627\u0626\u0647\u0645 \u0641\u064a\u0645\u0627 \u064a\u0623\u062a\u064a :<\/span><\/h4>\n
    \n
  • \n

    \u0623.\u062f. \u0645\u062d\u0645\u062f \u0641\u0631\u062c \u0634\u0630\u0631 (\u0631\u0626\u064a\u0633\u0627)<\/span><\/h4>\n<\/li>\n
  • \n

    \u0623.\u062f. \u0631\u0646\u0627 \u0645\u062c\u0627\u0647\u062f \u0639\u0628\u062f \u0627\u0644\u0644\u0647 (\u0639\u0636\u0648\u0627)<\/span><\/h4>\n<\/li>\n
  • \n

    \u0623.\u0645.\u062f. \u0646\u0627\u062f\u0631\u0629 \u0633\u0644\u0645\u0627\u0646 \u0645\u062d\u0645\u062f (\u0639\u0636\u0648\u0627)<\/span><\/h4>\n<\/li>\n
  • \n

    \u0623.\u0645.\u062f. \u0633\u0639\u0627\u062f \u062e\u0644\u064a\u0644 \u0625\u0628\u0631\u0627\u0647\u064a\u0645 (\u0639\u0636\u0648\u0627 \u0648 \u0645\u0634\u0631\u0641\u0627)<\/span><\/h4>\n<\/li>\n<\/ul>\n

    \"\"<\/h4>\n

    \u0648\u062a\u0647\u062f\u0641 \u0627\u0644\u0631\u0633\u0627\u0644\u0629 \u0627\u0644\u0649 :<\/span><\/h4>\n
      \n
    1. \n

      \u062c\u0645\u0639 \u0639\u0632\u0644\u0627\u062a \u0628\u0643\u062a\u064a\u0631\u064a\u0627 P.mirabilis<\/em> \u0645\u0646 \u0645\u0635\u0627\u062f\u0631 \u0627\u0644\u0633\u0631\u064a\u0631\u064a\u0629 (\u0627\u0644\u062d\u0631\u0648\u0642 \u0648 \u0627\u0644\u062c\u0631\u0648\u062d) \u0648 \u062a\u0634\u062e\u064a\u0635\u0647\u0627 \u0628\u0627\u0644\u0627\u0639\u062a\u0645\u0627\u062f \u0639\u0644\u0649 \u0627\u0644\u0635\u0641\u0627\u062a \u0627\u0644\u0645\u062c\u0647\u0631\u064a\u0629 \u0648 \u0627\u0644\u0645\u0632\u0631\u0639\u064a\u0629 \u0648 \u0627\u0644\u0643\u064a\u0645\u0648\u062d\u064a\u0648\u064a\u0629.<\/span><\/h4>\n<\/li>\n
    2. \n

      \u062a\u0623\u0643\u064a\u062f \u062a\u0634\u062e\u064a\u0635 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062c\u0647\u0627\u0632 Vitek-2 Compact System \u0648 \u0627\u0644\u062a\u0634\u062e\u064a\u0635 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u0628\u0648\u0633\u0627\u0637\u0629 \u0627\u0644\u062c\u064a\u0646 SrRNA<\/em> 16.<\/em><\/span><\/h4>\n<\/li>\n
    3. \n

      \u0627\u0644\u062a\u062d\u0631\u064a \u0639\u0646 \u062d\u0633\u0627\u0633\u064a\u0629 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0644\u0645\u062c\u0627\u0645\u064a\u0639 \u0645\u062e\u062a\u0644\u0641\u0629 \u0644\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0627\u062a\u064a\u0629.\u00a0<\/span><\/h4>\n<\/li>\n
    4. \n

      \u0627\u0644\u0643\u0634\u0641 \u0627\u0644\u0645\u0638\u0647\u0631\u064a \u0648 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u0639\u0646 \u0628\u0639\u0636 \u0639\u0648\u0627\u0645\u0644 \u0627\u0644\u0636\u0631\u0627\u0648\u0629 \u0628\u0643\u062a\u064a\u0631\u064a\u0627 P.mirabilis<\/em>.<\/span><\/h4>\n<\/li>\n
    5. \n

      \u0627\u0644\u0643\u0634\u0641 \u0627\u0644\u0645\u0638\u0647\u0631\u064a \u0639\u0646 \u0642\u0627\u0628\u0644\u064a\u0629 \u0639\u0632\u0644\u0627\u062a \u0628\u0643\u062a\u064a\u0631\u064a\u0627 P.mirabilis<\/em> \u0639\u0644\u0649 \u0627\u0646\u062a\u0627\u062c \u0627\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a Biofilm \u0648 \u0628\u0637\u0631\u064a\u0642\u0629 \u0627\u0644\u0635\u0641\u064a\u062d\u0629 \u0627\u0644\u0639\u064a\u0627\u0631\u064a\u0629 \u0627\u0644\u062f\u0642\u064a\u0642\u0629.<\/span><\/h4>\n<\/li>\n
    6. \n

      \u062f\u0631\u0627\u0633\u0640\u0640\u0640\u0640\u0629 \u062a\u0640\u0640\u0623\u062b\u0640\u0640\u064a\u0640\u0640\u0640\u0640\u0631 \u0627\u0644\u0645\u0633\u0640\u062a\u0640\u0640\u062e\u0640\u0640\u0644\u0640\u0640\u0640\u0640\u0640\u0635 \u0627\u0644\u0640\u0645\u0640\u0640\u0627\u0626\u0640\u0640\u0640\u0640\u064a \u0648 \u0627\u0644\u0643\u0640\u062d\u0640\u0640\u0648\u0644\u0640\u064a \u0648 \u0627\u0644\u0640\u0632\u064a\u0640\u0640\u0640\u062a \u0627\u0644\u0640\u0637\u0640\u0640\u064a\u0640\u0640\u0640\u0640\u0640\u0627\u0631 \u0644\u0646\u0640\u0628\u0640\u0640\u0627\u062a \u0627\u0644\u0646\u0640\u0639\u0640\u0646\u0640\u0640\u0640\u0640\u0640\u0640\u0627\u0639\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Mentha sepacia<\/em> \u060c \u0627\u0644\u0645\u0631\u0643\u0628 \u0627\u0644\u0637\u0628\u064a\u0639\u064a Menthol \u0648 \u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0641\u0636\u0629 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 (AgNPs) \u00a0\u00a0\u00a0\u00a0\u0639\u0644\u0649 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a \u0648 \u0627\u0646\u062a\u0627\u062c \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a Biofilm.<\/span><\/h4>\n<\/li>\n
    7. \n

      \u0627\u0644\u0643\u0634\u0641 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u0639\u0646 \u0628\u0639\u0636 \u0627\u0644\u062c\u064a\u0646\u0627\u062a (Ure C<\/em>\u060c Mrp A<\/em>\u060c<\/em> pmr I<\/em>) \u0648 \u0627\u0644\u062a\u064a \u0644\u0647\u0627 \u0639\u0644\u0627\u0642\u0629 \u0628\u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a Biofilm.<\/span><\/h4>\n<\/li>\n
    8. \n

      \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062a\u062a\u0627\u0628\u0639\u064a Sequencing \u0644\u0640 DNA \u0627\u0644\u062c\u064a\u0646\u064a\u0646 (Mrp A<\/em>\u060c pmr I<\/em> ).<\/span><\/h4>\n<\/li>\n<\/ol>\n

      \u0648 \u0634\u0645\u0644\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u062c\u0645\u0639 200 \u0639\u064a\u0646\u0629 \u062a\u0639\u0648\u062f \u0627\u0644\u0649 \u0627\u0644\u062c\u0646\u0633 Proteus<\/em> \u0645\u0646 \u0625\u0635\u0627\u0628\u0627\u062a \u0627\u0644\u062d\u0631\u0648\u0642 \u0648 \u0627\u0644\u062c\u0631\u0648\u062d \u062a\u0636\u0645\u0646\u062a (125) \u0639\u064a\u0646\u0629 \u0645\u0646 \u0627\u0644\u062d\u0631\u0648\u0642 \u0648 (75) \u0639\u064a\u0646\u0629 \u0645\u0646 \u0627\u0644\u062c\u0631\u0648\u062d \u0648 \u0645\u0646 \u0639\u0640\u062f\u0629 \u0645\u0633\u062a\u0634\u0641\u064a\u0627\u062a \u0641\u064a \u0645\u062f\u064a\u0646\u0640\u0629 \u0628\u063a\u0640\u062f\u0627\u062f \u0647\u064a: \u0645\u0633\u062a\u0634\u0641\u0649 \u0627\u0644\u0643\u0640\u0646\u062f\u064a \u0627\u0644\u062a\u0639\u0644\u064a\u0645\u064a \u060c \u0645\u062c\u0645\u0639 \u0645\u0633\u062a\u0634\u0641\u064a\u0627\u062a \u0645\u062f\u064a\u0646\u0629 \u0627\u0644\u0637\u0628 (\u0645\u0633\u062a\u0634\u0641\u0649 \u0627\u0644\u062d\u0631\u0648\u0642 \u0627\u0644\u062a\u062e\u0635\u0635\u064a \u060c \u0645\u0633\u062a\u0634\u0641\u0649 \u063a\u0627\u0632\u064a \u0627\u0644\u062d\u0631\u064a\u0631\u064a \u0627\u0644\u062a\u0639\u0644\u064a\u0645\u064a \u060c \u0645\u0633\u062a\u0634\u0641\u0649 \u0628\u063a\u062f\u0627\u062f \u0627\u0644\u062a\u0639\u0644\u064a\u0645\u064a \u060c \u0645\u0633\u062a\u0634\u0641\u0649 \u0627\u0644\u0637\u0641\u0644 \u0627\u0644\u0645\u0631\u0643\u0632\u064a \u00a0\u0648 \u0627\u0644\u0645\u062e\u062a\u0628\u0631\u0627\u062a \u0627\u0644\u062a\u0639\u0644\u064a\u0645\u064a\u0629 ) \u060c \u0644\u0644\u0645\u0640\u062f\u0629 \u0645\u0646 1\/12\/2018 \u0627\u0644\u0649 1\/3\/2019 . \u0634\u062e\u0635\u062a \u062c\u0645\u064a\u0639 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0639\u062a\u0645\u0627\u062f\u0627\u064b \u0639\u0644\u0649 \u0635\u0641\u0627\u062a\u0647\u0627 \u0627\u0644\u0645\u0638\u0647\u0631\u064a\u0629 \u0648 \u0627\u0644\u0627\u062e\u062a\u0628\u0627\u0631\u0627\u062a \u0627\u0644\u0643\u064a\u0645\u0648\u062d\u064a\u0648\u064a\u0629 \u060c \u0648 \u062a\u0645 \u0627\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 31 \u0639\u0632\u0644\u0629 \u062a\u0639\u0648\u062f \u0644\u0628\u0643\u062a\u0631\u064a\u0627 Proteus mirabilis<\/em> \u0628\u0639\u062f \u0627\u0646 \u062a\u0645 \u062a\u0623\u0643\u064a\u062f\u0647\u0627 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0646\u0638\u0627\u0645 Vitek 2 Compact System \u0648 \u0627\u0644\u0628\u0627\u062f\u0626 \u0627\u0644\u0646\u0648\u0639\u064a \u0644\u0644\u062c\u064a\u0646 16S rRNA<\/em> \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062a\u0642\u0646\u064a\u0629 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 PCR\u00a0 \u0625\u0630 \u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u0634\u062e\u064a\u0635 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u0627\u0646 \u062c\u0645\u064a\u0639 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0627\u0645\u062a\u0644\u0643 \u0627\u0644\u062c\u064a\u0646 16S rRNA <\/em>.<\/span><\/h4>\n

      \u0628\u064a\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0641\u062d\u0635 \u062d\u0633\u0627\u0633\u064a\u0629 \u0639\u0632\u0644\u0627\u062a P.mirabilis<\/em> (31\u0639\u0632\u0644\u0629) \u0644\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0628\u0637\u0631\u064a\u0642\u0629 \u0627\u0646\u062a\u0634\u0627\u0631 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u0627\u0644\u0642\u0631\u0635 Kirby-Bauer Method \u0627\u0646\u0647\u0627 \u062a\u0645\u062a\u0644\u0643 \u0627\u0644\u0645\u0642\u0627\u0648\u0645\u0629 \u0627\u0644\u0645\u062a\u0639\u062f\u062f\u0629 \u0644\u0640\u0640 (2-12) \u0645\u0646 \u0627\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0627\u0644\u0645\u062e\u062a\u0628\u0631\u0629\u060c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0625\u0630 \u0648\u062c\u062f \u0627\u0646 \u0627\u0642\u0644 \u0645\u0639\u062f\u0644 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0639\u0640\u0640\u0640\u0640\u0640\u0632\u0644\u0627\u062a \u0628\u0643\u062a\u064a\u0631\u064a\u0627 P.mirabilis<\/em> \u0643\u0627\u0646 \u0644\u0645\u0636\u0627\u062f\u0627\u062a Norfloxacin (9.6%) \u064a\u0644\u064a\u0647 \u0645\u0636\u0627\u062f \u00a0Levofloxacin (13%) \u060c \u00a0Imipenem (16.1%) \u060c Ciprofloxacin (32.2%)\u00a0 \u0648 Cefoxitin (38.7%) \u0628\u064a\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0643\u0634\u0641 \u0627\u0644\u0645\u0638\u0647\u0631\u064a \u0639\u0646 \u0639\u0648\u0627\u0645\u0644 \u0636\u0631\u0627\u0648\u0629 \u0628\u0643\u062a\u064a\u0631\u064a\u0627 P.mirabilis<\/em> \u0625\u0630 \u0627\u0646\u0647\u0627 \u0627\u0646\u062a\u062c\u062a \u0643\u0644\u0627\u064b \u0645\u0646 \u0627\u0646\u0632\u064a\u0645 \u0627\u0644\u064a\u0648\u0631\u064a\u0632 \u0648 \u0627\u0644\u0647\u064a\u0645\u0648\u0644\u0627\u064a\u0633\u064a\u0646 \u0648 \u0627\u0644\u062c\u064a\u0644\u0627\u062a\u064a\u0646\u064a\u0632 \u0628\u0646\u0633\u0628\u0629 (100%) \u0648 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0632 (77.5%) \u0627\u0645\u0627 \u0628\u0627\u0644\u0646\u0633\u0628\u0629 \u0623\u0646\u0632\u064a\u0645 \u0627\u0644\u062f\u0646\u0627\u064a\u0632 \u0641\u0642\u062f \u0627\u0646\u062a\u062c\u062a\u0647 \u0628\u0646\u0633\u0628\u0629 (93.5%) \u0648 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0627\u0644\u062d\u0627\u0645\u0644\u0629 \u0644\u0644\u062d\u062f\u064a\u062f \u0643\u0627\u0646\u062a \u0628\u0646\u0633\u0628\u0629 (16%).<\/span><\/h4>\n

      \u00a0\u00a0\u00a0\u00a0 \u0623\u0638\u0647\u0631\u062a \u0637\u0631\u064a\u0642\u0629 \u0627\u0644\u0635\u0641\u064a\u062d\u0629 \u0627\u0644\u0639\u064a\u0627\u0631\u064a\u0629 \u0627\u0644\u062f\u0642\u064a\u0642\u0629 Microtiter Plate \u0627\u0646 \u062c\u0645\u064a\u0639 \u0627\u0644\u0639\u0632\u0644\u0627\u062a (100%) \u0643\u0648\u0646\u062a \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0648 \u0628\u062f\u0631\u062c\u0627\u062a \u0645\u062e\u062a\u0644\u0641\u0629 \u062a\u0645 \u062f\u0631\u0627\u0633\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0629 \u062e\u0635\u0640\u0627\u0626\u0640\u0640\u0640\u0640\u0635 \u0627\u0644\u0641\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0636\u0640\u0629 \u0627\u0644\u0646\u0640\u0640\u0627\u0646\u0640\u0640\u0640\u0640\u0648\u064a\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0629 Silver Nanoparticles \u0644\u0644\u062a\u0623\u0643\u062f \u0645\u0646 \u062d\u0640\u0640\u062c\u0640\u0640\u0640\u0640\u0640\u0645 \u062c\u0632\u064a\u0626\u0627\u062a\u0647\u0627 \u0628\u0627\u0644\u0627\u0639\u062a\u0645\u0627\u062f \u0639\u0644\u0649 \u0623\u062c\u0647\u0632\u0629 \u0627\u0644\u062a\u0634\u062e\u064a\u0635 \u0627\u0644\u0631\u0626\u064a\u0633\u064a\u0629 \u0627\u0644\u0645\u062a\u0645\u062b\u0644\u0629 \u0628\u062c\u0647\u0627\u0632 \u062d\u064a\u0648\u062f \u0627\u0644\u0627\u0634\u0639\u0629 \u0627\u0644\u0633\u064a\u0646\u064a\u0629 X-Ray Diffraction \u0648 \u0627\u0644\u0640\u0640\u0640\u0645\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u062c\u0640\u0640\u0640\u0640\u0640\u0647\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0631 \u0627\u0644\u0627\u0644\u0643\u0640\u0640\u0640\u0640\u0640\u062a\u0640\u0640\u0640\u0631\u0648\u0646\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u064a \u0627\u0644\u0640\u0645\u0640\u0640\u0627\u0633\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u0640\u062d \u0630\u0648 \u0627\u0644\u0627\u0646\u0628\u0640\u0640\u0640\u0639\u0640\u0640\u0627\u062b \u0627\u0644\u0643\u0640\u0640\u0627\u0645\u0640\u0640\u0640\u0644 Scanning Electron Microscopy Field Emission \u00a0\u0648 \u0645\u062c\u0647\u0631 \u0627\u0644\u0642\u0640\u0640\u0648\u0649 \u0627\u0644\u0630\u0631\u064a\u0629 Atomic Force Microscopy (AFM) \u0627\u0634\u0627\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0646 \u0627\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u0645\u0641\u062d\u0648\u0635\u0629 \u0647\u064a \u062c\u0632\u064a\u0626\u0627\u062a \u0627\u0644\u0641\u0636\u0629 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 (AgNPs) \u0628\u0635\u0648\u0631\u0629 \u0646\u0642\u064a\u0629 \u0648 \u0628\u0623\u0634\u0643\u0627\u0644 \u0645\u062e\u062a\u0644\u0641\u0629 \u0648 \u0627\u062d\u062c\u0627\u0645 \u062a\u062a\u0631\u0627\u0648\u062d \u0628\u064a\u0646 18-41 \u0646\u0627\u0646\u0648\u0645\u064a\u062a\u0631. \u062f\u0631\u0633 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0645\u0633\u062a\u062e\u0644\u0635 \u0627\u0644\u0645\u0627\u0626\u064a \u0648 \u0627\u0644\u0643\u062d\u0648\u0644\u064a \u0644\u0646\u0628\u0627\u062a \u0627\u0644\u0646\u0639\u0646\u0627\u0639 Mentha sepacia<\/em> \u0648 \u0627\u0644\u0645\u0631\u0643\u0628 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u00a0 \u0644\u0644\u0646\u0639\u0646\u0627\u0639 Menthol \u0648 \u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0641\u0636\u0629 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 (AgNPs) \u0644\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0645\u0646\u062a\u062e\u0628\u0629 (\u0627\u0631\u0628\u0639 \u0639\u0632\u0644\u0627\u062a)\u00a0 \u0627\u0644\u0623\u0643\u062b\u0631 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0648 \u0627\u0644\u0634\u062f\u064a\u062f\u0629 \u0627\u0644\u062a\u0643\u0648\u064a\u0646 \u0644\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0639\u0644\u0649 \u062a\u062b\u0628\u064a\u0637 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a \u0648 \u062a\u062b\u0628\u064a\u0637 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a Biofilm \u060c \u0625\u0630 \u0628\u064a\u0646\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0639\u062f\u0645 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0645\u0633\u062a\u062e\u0644\u0635 \u0627\u0644\u0645\u0627\u0626\u064a \u0648 \u0627\u0644\u0643\u062d\u0648\u0644\u064a \u0644\u0646\u0628\u0627\u062a \u0627\u0644\u0646\u0639\u0646\u0627\u0639 \u0641\u064a \u062a\u062b\u0628\u064a\u0637 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a \u0648 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a Biofilm \u0641\u064a \u062d\u064a\u0646 \u0623\u0638\u0647\u0631\u062a \u0627\u0646 \u0627\u0644\u0632\u064a\u062a \u0627\u0644\u0637\u064a\u0627\u0631 \u0644\u0646\u0628\u0627\u062a Mentha sepacia<\/em> \u00a0\u0648 \u0627\u0644\u0645\u0631\u0643\u0628 \u0627\u0644\u0637\u0628\u064a\u0639\u064a Menthol \u0648 \u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0641\u0636\u0629 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 (AgNPs) \u0643\u0627\u0646\u062a \u0641\u0639\u0627\u0644\u0629 \u062c\u062f\u0627\u064b \u0641\u064a \u062a\u062b\u0628\u064a\u0637 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a \u0648 \u062a\u062b\u0628\u064a\u0637 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a Biofilm \u0648 \u0628\u0646\u0633\u0628 \u0645\u062e\u062a\u0644\u0641\u0629 \u062a\u0628\u0639\u0627\u064b \u0627\u0644\u0649 \u0645\u0635\u062f\u0631 \u0627\u0644\u0639\u0632\u0644\u0629 \u0648\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0645\u0627\u062f\u0629. \u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0641\u062d\u0635 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u0644\u0644\u062c\u064a\u0646\u0627\u062a (pmr I , mrp A ,Ure C <\/em>) \u0627\u0644\u0645\u0633\u0624\u0648\u0644\u0629 \u0627\u0648 \u0627\u0644\u062a\u064a \u0644\u0647\u0627 \u062f\u0648\u0631 \u0641\u064a \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a Biofilm Formation \u0627\u0646 \u0639\u0632\u0644\u0627\u062a \u0628\u0643\u062a\u064a\u0631\u064a\u0627 P.mirabilis<\/em> \u0627\u0645\u062a\u0644\u0643\u062a\u0647\u0627 \u0628\u0646\u0633\u0628\u0629 ( 93.5% , 42% \u060c 90.3%) \u0639\u0644\u0649 \u0627\u0644\u062a\u0648\u0627\u0644\u064a \u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u062a\u062a\u0627\u0628\u0639\u064a DNA sequencing \u0644\u0644\u0628\u0648\u0627\u062f\u0626 \u0627\u0644\u0627\u0645\u0627\u0645\u064a\u0629 Forward\u00a0 \u00a0\u0648 \u0627\u0644\u0639\u0643\u0633\u064a\u0629\u00a0 Reverse\u00a0 \u0644\u0644\u062c\u0640\u064a\u0646\u0640\u0640\u0627\u062a (pmr I , mrp A<\/em>) \u0644\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u062b\u0644\u0627\u062b\u0629 \u0627\u0644\u0645\u0646\u062a\u062e\u0628\u0629 \u060c \u0625\u0630 \u0643\u0627\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u062a\u062a\u0627\u0628\u0639\u064a \u0645\u062a\u0637\u0627\u0628\u0642\u0629 \u0644\u0644\u062c\u064a\u0646 mrp A<\/em> \u0644\u062c\u0645\u064a\u0639 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0645\u0646\u062a\u062e\u0628\u0629 \u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0628\u0627\u0644\u0633\u0644\u0627\u0644\u0627\u062a \u0627\u0644\u0645\u0631\u062c\u0639\u064a\u0629 \u0628\u064a\u0646\u0645\u0627 \u0628\u0627\u0644\u0646\u0633\u0628\u0629 \u0627\u0644\u062c\u064a\u0646 pmr I<\/em> \u0641\u0642\u062f \u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0648\u062c\u0648\u062f \u0637\u0641\u0631\u062a\u064a\u0646 \u0645\u0646 \u0627\u0644\u0646\u0648\u0639 \u0627\u0644\u0635\u0627\u0645\u062a\u0629 Silent Mutation.<\/span><\/h4>\n

      \u00a0\u00a0\u00a0 \u0648\u062e\u0644\u0635 \u0627\u0644\u0637\u0627\u0644\u0628 \u0641\u064a \u062e\u062a\u0627\u0645 \u0631\u0633\u0627\u0644\u062a\u0647 \u0627\u0644\u0649 \u0627\u0644\u062a\u0648\u0635\u064a\u0627\u062a \u0627\u0644\u0627\u062a\u064a\u0629 :<\/span><\/h4>\n
        \n
      • \n

        \u0627\u062c\u0631\u0627\u0621 \u062f\u0631\u0627\u0633\u0627\u062a \u0644\u0640\u0642\u064a\u0627\u0633 \u0645\u0633\u062a\u0648\u0649 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0627\u0644\u0646\u0627\u062a\u062c \u0639\u0646 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0632\u064a\u062a \u0627\u0644\u0637\u064a\u0627\u0631 \u0644\u0646\u0628\u0627\u062a \u0627\u0644\u0646\u0639\u0646\u0627\u0639 sepatia<\/em> \u0648 \u0627\u0644\u0645\u0631\u0643\u0628 \u0627\u0644\u0637\u0628\u064a\u0639\u064a Menthol \u0648 \u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0641\u0636\u0629 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 AgNPs .<\/span><\/h4>\n<\/li>\n
      • \n

        \u0627\u062c\u0631\u0627\u0621 \u062f\u0631\u0627\u0633\u0627\u062a \u062d\u0648\u0644 \u0627\u0644\u0627\u0644\u064a\u0627\u062a \u0627\u0644\u062a\u064a \u062a\u062b\u0628\u0637 \u0627\u0648 \u062a\u062d\u0641\u0632 \u0646\u0645\u0648 \u0628\u0643\u062a\u064a\u0631\u064a\u0627 mirabilis<\/em> .<\/span><\/h4>\n<\/li>\n
      • \n

        \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u064a\u0629 \u062a\u0634\u0643\u0644 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a Biofilm \u0648 \u062a\u062b\u0628\u064a\u0637\u0647 \u062f\u0627\u062e\u0644 \u062c\u0633\u0645 \u0627\u0644\u0643\u0627\u0626\u0646 In Vivo<\/em> .<\/em><\/span><\/h4>\n<\/li>\n
      • \n

        \u0627\u062c\u0631\u0627\u0621 \u062f\u0631\u0627\u0633\u0627\u062a \u0645\u0638\u0647\u0631\u064a\u0629 \u0648 \u062c\u0632\u064a\u0626\u064a\u0629 \u0639\u0646 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0632\u064a\u062a \u0627\u0644\u0637\u064a\u0627\u0631 \u0644\u0646\u0628\u0627\u062a \u0627\u0644\u0646\u0639\u0646\u0627\u0639 sepatia<\/em> \u0648 \u0627\u0644\u0645\u0631\u0643\u0628 \u0627\u0644\u0637\u0628\u064a\u0639\u064a Menthol \u0648 \u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0641\u0636\u0629 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 AgNPs \u0639\u0644\u0649 \u0639\u0648\u0627\u0645\u0644 \u0636\u0631\u0627\u0648\u0629 \u0628\u0643\u062a\u064a\u0631\u064a\u0627 P.mirabilis<\/em> \u0627\u0644\u0627\u062e\u0631\u0649 \u0645\u062b\u0644 \u0627\u0644\u0647\u064a\u0645\u0648\u0644\u0627\u064a\u0633\u064a\u0646 Hemolysin \u0648 \u0627\u0646\u0632\u064a\u0645 \u0627\u0644\u064a\u0648\u0631\u064a\u0632 Urease \u0648 \u0627\u0646\u0632\u064a\u0645 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0632 Protease.<\/span><\/h4>\n<\/li>\n
      • \n

        \u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0627\u0644\u0645\u0636\u0627\u062f \u0627\u0644\u062d\u064a\u0648\u064a Norfloxacin \u0641\u064a \u0639\u0644\u0627\u062c \u0627\u0644\u0625\u0635\u0627\u0628\u0627\u062a \u0627\u0644\u0646\u0627\u062a\u062c\u0629 \u0639\u0646 \u0628\u0643\u062a\u064a\u0631\u064a\u0627 P.mirabilis <\/em>\u0627\u0644\u0645\u0639\u0632\u0648\u0644\u0629 \u0645\u0646 \u0627\u0644\u062d\u0631\u0648\u0642 \u0648 \u0627\u0644\u062c\u0631\u0648\u062d \u060c \u0627\u0630 \u0623\u0638\u0647\u0631\u062a \u0627\u0642\u0644 \u0645\u0639\u062f\u0644 \u0645\u0642\u0627\u0648\u0645\u0629 (9.6%).<\/span><\/h4>\n
        \n

        Thesis title: study effect for some biofilm inhibitors in Proteus mirabilisBacteria isolated from Burns and Wounds<\/span><\/h3>\n

        By Amed.W.Aziz<\/span><\/h3>\n

        Supervised by Souad Khalil Ibrahim<\/span><\/h3>\n

        Aim of study<\/span><\/h3>\n

        -Collections of P.mirabilis isolates were collected from clinical sources (burns and wounds) and diagnosed based on microbiological characteristic, culture and biochemical characteristics\u00a0 \u00a0<\/span><\/h3>\n

        Confirmation of the diagnosis of bacterial isolates using the Vitek-2 Compact System and the molecular diagnosis by gene 16SrRNA<\/span><\/h3>\n

        – Investigate the sensitivity of bacterial isolates to different groups of antibiotics.<\/span><\/h3>\n

        – Phenotypic and molecular detection of some virulence factors for P.mirabilis.<\/span><\/h3>\n

        <\/h3>\n

        – Phenotypic detection of susceptibility to isolates of P.mirabilis bacteria on biofilm production by Microtiter plate method.<\/span><\/h3>\n

        – Study the effect of aqueous and alcoholic extracts and volatile oils of mint plant Mentha sepacia, natural compound Menthol and silver nanoparticles (AgNPs) on bacterial growth and biofilm production.<\/span><\/h3>\n

        <\/h3>\n

        -Molecular detection of some genes (Ure C, Mrp A, pmrI) that are related to Biofilm formation.<\/span><\/h3>\n

        <\/h3>\n

        – Study of Sequencing of the tow genes DNA (Mrp A, pmr I).<\/span><\/h3>\n

        Abstract<\/span><\/h3>\n

        \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 The study included the collection of 200 specimens belonging to the genus Proteus from burns and wounds infection. It included (125) samples of burns and (75) samples of wounds and from several hospitals in Baghdad governorate: the Al-Kindy Teaching Hospital, the Medical City Hospital Complex (the Burns Specialist Hospital, Ghazi Al – Hariri Teaching Hospital, Baghdad Teaching Hospital, Central Child Hospital and Educational Laboratories), for the period from 1\/12\/2018 to 1\/3\/2019.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/h3>\n

        \u00a0\u00a0\u00a0\u00a0\u00a0 All isolates were diagnosed based on their phenotypic and biochemical tests, and (31 isolates) belonging to Proteus mirabilis were obtained after they were confirmed using the Vitek 2 Compact System and the 16S rRNA gene using the PCR technique, as the results of the molecular diagnosis showed that all Bacterial isolates possessed the 16S rRNA gene.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/h3>\n

        \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 The results of the of the sensitivity test of P.mirabilis isolates (31) isolates to the antibiotics using the Kirby-Bauer Method diffusion showed that they possessed the multiple resistance of (2-12) of the tested antibiotics, as it was found that the lowest rate of resistance to the isolates of P.mirabilis was to Norfloxacin ( 9.6%) is followed by Levofloxacin (13%), Imipenem, Ciprofloxacin (32.2%), and Cefoxitin(16.1%) .\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0(38.7%)<\/span><\/h3>\n

        The results of the phenotypic detection showed the virulence factors of P.mirabilis bacteria as they produced both urease enzyme, Hemolysin and Gelatinase by (100%) and Protease (77.5%). As for the enzyme DNase it produced at a rate (93.5%) and Siderophores compounds were at a rate (16%).\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/h3>\n

        \u00a0\u00a0\u00a0 As for the production of the biofilm, the method of culture in Congo Red agar (CRD) showed that 27 isolates (87%) formed the biofilm in different degrees, while the Microtiter plate (MTP) showed that all isolates (100%) formed the Biofilm and to varying degrees.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/h3>\n

        \u00a0\u00a0\u00a0\u00a0 The characteristics of Silver Nanoparticles (AgNPs) have been studied to confirm the size of their particles by relying on the main diagnostic devices represented by the X-Ray Diffraction and the Electron-magnetic field (AFM) and Field Emission Scanning Electron Microscopy (FESEM). The results indicated that the examined material is pure silver nanoparticles (AgNPs) various forms and sizes ranging from 18-41 Nanometers.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/h3>\n

        \u00a0\u00a0\u00a0\u00a0 Study the effect of the Aqueous and Alcoholic extract of the Mentha sepacia plant, the\u00a0 Natural active compound of the Menthol mint and the silver nanoparticles (AgNPs) of the selected (4 isolates) that are more resistant to the antibiotic and highly-formed biofilm on the inhibition of bacterial growth and the inhibition of the biofilm, as the results showed The ineffectiveness of the aqueous and alcoholic extract of the mint plant on the inhibition of bacterial growth and the biofilm membrane, while it showed that the volatile oil of Mentha sepacia plant, Natural compound Menthol and silver nanoparticles (AgNPs) were very effective in inhibiting bacterial growth and inhibition of the Biofilm and in proportions different depending on the source of isolation and substance concentration\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/span><\/h3>\n

        \u00a0\u00a0\u00a0\u00a0 The results of molecular detection of genes (pmr I, mrp A, Ure C) responsible or having a role in the formation of Biofilm showed that\u00a0\u00a0\u00a0\u00a0 isolates of P.mirabilis bacteria possessed it by (90.3% ,42% ,90.3%) respectively. \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/h3>\n

        \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 The results of the DNA sequencing of the forward and Reverse genes of the genes (pmr I, mrp A) for the three selected isolates were shown, as the results of the analysis were identical to the pmr I gene for all the selected isolates compared to the reference strains while for the mrp A gene, the results showed that there is one mutation of the type Silent Mutation\u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/h3>\n

        Recommendation<\/span><\/h3>\n

        -Conducting studies to measure the level of gene expression resulting from the effect of the Volatile oil of the mint plant, M.spatcia, the natural compound Menthol and the silver nanoparticles (AgNPs<\/span>\u00a0 \u00a0\u00a0<\/span><\/h3>\n

        -Conducting studies on mechanisms that inhibit or stimulate the growth of P.mirabilis bacteria<\/span><\/h3>\n

        -study the mechanism of biofilm formation and inhibition inside the body of the object In Vivo<\/span><\/h3>\n

        – Conduct studies Molecular and phenotypic properties of the effect ofM. sepatcia, Menthol, and AgNPs on other virulence factors P.mirabilis bacteria such as Hemolysin, Urease and Protease.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/h3>\n

        -The use of the antibiotic Norfloxacin in treating infections caused by bacteria P.mirabilis isolated from burns and wounds, as it showed the lowest resistance rate (9.6%<\/span><\/h3>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"

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