{"id":32853,"date":"2020-08-16T12:57:15","date_gmt":"2020-08-16T09:57:15","guid":{"rendered":"http:\/\/ihcoedu.uobaghdad.edu.iq\/?p=32853"},"modified":"2020-09-19T21:27:41","modified_gmt":"2020-09-19T18:27:41","slug":"%d9%82%d8%b3%d9%85-%d8%b9%d9%84%d9%88%d9%85-%d8%a7%d9%84%d8%ad%d9%8a%d8%a7%d8%a9-%d9%8a%d9%86%d8%a7%d9%82%d8%b4-%d8%b1%d8%b3%d8%a7%d9%84%d8%a9-%d9%85%d8%a7%d8%ac%d8%b3%d8%aa%d9%8a%d8%b1-%d8%aa-14","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=32853","title":{"rendered":"\u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u064a\u0646\u0627\u0642\u0634 \u0631\u0633\u0627\u0644\u0629 \u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u062a\u0628\u062d\u062b \u0641\u064a \u0627\u0644\u062a\u062e\u0644\u064a\u0642 \u0627\u0644\u062d\u064a\u0648\u064a \u0644\u062c\u0632\u064a\u0626\u0627\u062a \u0627\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u0648\u0645 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\/><\/strong><\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\"><strong>\u0646\u0627\u0642\u0634 \u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u062a\u0631\u0628\u064a\u0629 \u0644\u0644\u0639\u0644\u0648\u0645 \u0627\u0644\u0635\u0631\u0641\u0629 ( \u0627\u0628\u0646 \u0627\u0644\u0647\u064a\u062b\u0645 ) \u0631\u0633\u0627\u0644\u0629 \u0627\u0644\u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u0627\u0644\u0645\u0648\u0633\u0648\u0645\u0629 ( <\/strong>\u00a0\u0627\u0644\u062a\u062e\u0644\u064a\u0642 \u0627\u0644\u062d\u064a\u0648\u064a \u0644\u062c\u0632\u064a\u0626\u0627\u062a \u0627\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a \u0645\u0646 \u0628\u0643\u062a\u064a\u0631\u064a\u0627<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">\u0627\u0644\u0643\u0644\u064a\u0628\u0633\u064a\u0644\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0627\u0644\u0645\u0639\u0632\u0648\u0644\u0629 \u0645\u062d\u0644\u064a\u0627 ) \u0644\u0644\u0637\u0627\u0644\u0628 ( \u062d\u0633\u064a\u0646 \u062d\u064a\u062f\u0631 \u0645\u0638\u0641\u0631 \u0639\u0628\u062f \u0627\u0644\u062d\u0645\u064a\u062f ) \u0627\u0644\u062a\u064a \u0627\u0646\u062c\u0632\u0647\u0627 \u062a\u062d\u062a \u0627\u0634\u0631\u0627\u0641 \u0627\u0644\u062a\u062f\u0631\u064a\u0633\u064a \u0641\u064a \u0627\u0644\u0642\u0633\u0645 ( \u0623.\u0645.\u062f. \u0639\u0635\u0627\u0645 \u062c\u0627\u0633\u0645 \u0627\u0644\u062e\u0644\u064a\u0641\u0627\u0648\u064a ) \u0648\u0646\u0648\u0642\u0634\u062a \u0645\u0646 \u0642\u0628\u0644 \u0644\u062c\u0646\u0629 \u0627\u0644\u0645\u0646\u0627\u0642\u0634\u0629 \u0627\u0644\u062a\u064a \u062a\u0623\u0644\u0641\u062a \u0645\u0646 \u0627\u0644\u0627\u0639\u0636\u0627\u0621 \u0627\u0644\u0645\u062f\u0631\u062c\u0629 \u0627\u0633\u0645\u0627\u0626\u0647\u0645 \u0641\u064a\u0645\u0627 \u064a\u0623\u062a\u064a :<\/span><\/h4>\n<ol style=\"text-align: justify\">\n<li>\n<h4><span style=\"color: #000080\">\u0623.\u062f. \u0644\u0645\u0649 \u0639\u0628\u062f \u0627\u0644\u0647\u0627\u062f\u064a \u0632\u0648\u064a\u0646 \u00a0(\u0631\u0626\u064a\u0633\u0627)<\/span><\/h4>\n<\/li>\n<li>\n<h4><span style=\"color: #000080\">\u0623.\u0645.\u062f. \u0644\u064a\u062b \u0627\u062d\u0645\u062f \u064a\u0639\u0642\u0648\u0628 ( \u0639\u0636\u0648\u0627 )<\/span><\/h4>\n<\/li>\n<li>\n<h4><span style=\"color: #000080\">\u0623.\u0645.\u062f. \u0646\u0647\u0627\u064a\u0629 \u062d\u0643\u0645\u062a \u0632\u0643\u064a ( \u0639\u0636\u0648\u0627 )<\/span><\/h4>\n<\/li>\n<li>\n<h4><span style=\"color: #000080\">\u0623.\u0645.\u062f. \u0639\u0635\u0627\u0645 \u062c\u0627\u0633\u0645 \u0627\u0644\u062e\u0644\u064a\u0641\u0627\u0648\u064a ( \u0639\u0636\u0648\u0627 \u0648\u0645\u0634\u0631\u0641\u0627 )<\/span><\/h4>\n<\/li>\n<\/ol>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-32857\" src=\"https:\/\/ihcoedu.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/27\/2020\/08\/IMG_9718.jpg\" alt=\"\" width=\"800\" height=\"533\" srcset=\"https:\/\/ihcoedu.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/27\/2020\/08\/IMG_9718.jpg 800w, https:\/\/ihcoedu.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/27\/2020\/08\/IMG_9718-300x200.jpg 300w, https:\/\/ihcoedu.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/27\/2020\/08\/IMG_9718-768x512.jpg 768w\" sizes=\"auto, (max-width: 800px) 100vw, 800px\" \/><\/p>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">\u0647\u062f\u0641\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u0625\u0644\u0649 \u062f\u0631\u0627\u0633\u0629 \u062a\u0623\u062b\u064a\u0631\u0627\u062a \u0627\u0644\u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0644\u0627\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0643\u0645\u0636\u0627\u062f \u0644\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0648\u0645\u0636\u0627\u062f \u0644\u0639\u0648\u0627\u0645\u0644 \u0627\u0644\u0638\u0631\u0627\u0648\u0629 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 ( \u00a0\u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \/\u0627\u0644\u0628\u0627\u064a\u0648\u0641\u0644\u0645 ) \u00a0\u0648\u0645\u0636\u0627\u062f \u0644\u0644\u0633\u0631\u0637\u0627\u0646 \u060c \u0648\u0647\u0630\u0627 \u0627\u0644\u0647\u062f\u0641 \u064a\u062a\u0637\u0644\u0628 \u0627\u0644\u0625\u062c\u0631\u0627\u0621 \u0627\u0644\u062a\u0627\u0644\u064a: &#8211;<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">1- \u0639\u0632\u0644 \u0648\u062a\u0634\u062e\u064a\u0635 \u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0627\u0644\u0643\u0644\u064a\u0628\u0633\u064a\u0644\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0645\u0646 \u0639\u064a\u0646\u0627\u062a \u0633\u0631\u064a\u0631\u064a\u0629 \u0645\u062e\u062a\u0644\u0641\u0629 \u0648\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0623\u0633\u0627\u0644\u064a\u0628 \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">(\u0627\u0644\u0632\u0631\u0627\u0639\u0629 \u0641\u064a \u0627\u0644\u0648\u0633\u0637 \u0627\u0644\u0627\u0646\u062a\u0642\u0627\u0626\u064a \u060c \u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u0643\u064a\u0645\u0648\u062d\u064a\u0648\u064a\u0629 \u0648\u062c\u0647\u0627\u0632 \u00a0vitek-2.)<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">2- \u062a\u062d\u062f\u064a\u062f \u00a0\u062d\u0633\u0627\u0633\u064a\u0629 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0627\u062a\u062c\u0627\u0647 \u0627\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0636\u062f \u0641\u0626\u0627\u062a \u0645\u062a\u0639\u062f\u062f\u0629 \u0645\u0646 \u0627\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">3- \u062a\u062d\u062f\u064a\u062f \u00a0\u0642\u0627\u0628\u0644\u064a\u0629 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0639\u0644\u0649 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a ( \u00a0(biofilm \u00a0\u0644\u0643\u0644 \u0645\u0646 \u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0627\u0644\u0633\u0627\u0644\u0628\u0629 \u0644\u0635\u0628\u063a\u0629 \u0627\u0644\u0643\u0631\u0627\u0645<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">( \u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0627\u0644\u0643\u0644\u064a\u0628\u0633\u064a\u0644\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0648 \u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0627\u0644\u0632\u0627\u0626\u0641\u0629 \u0627\u0644\u0632\u0646\u062c\u0627\u0631\u064a\u0629 ) \u0648\u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0627\u0644\u0645\u0648\u062c\u0628\u0629 \u0644\u0635\u0628\u063a\u0647 \u0643\u0631\u0627\u0645 ( \u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0627\u0644\u0645\u0643\u0648\u0631\u0627\u062a \u0627\u0644\u0639\u0646\u0642\u0648\u062f\u064a\u0629 \u0627\u0644\u0630\u0647\u0628\u064a\u0629 \u0627\u0644\u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0645\u0636\u0627\u062f \u0627\u0644\u062d\u064a\u0648\u064a \u0627\u0644\u0627\u0648\u0643\u0633\u064a\u0644\u064a\u0646 \u0648 \u0627\u0644\u0641\u0627\u0646\u0643\u0648\u0645\u0627\u064a\u0633\u064a\u0646 ) \u0628\u0627\u0633\u062a\u062e\u0627\u062f\u0645 \u0637\u0631\u064a\u0642\u0629 \u0627\u0644\u0623\u0644\u0648\u0627\u062d \u0627\u0644\u062f\u0642\u064a\u0642\u0629.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">4- \u0627\u0644\u062a\u062e\u0644\u064a\u0642 \u0627\u0644\u062d\u064a\u0648\u064a \u0644\u0644\u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0623\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0645\u0633\u062a\u062e\u0644\u0635 \u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0627\u0644\u0643\u0644\u064a\u0628\u0633\u064a\u0644\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">5- \u062a\u0648\u0635\u064a\u0641 \u0627\u0644\u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0644\u0623\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u062c\u0647\u0632\u0629 \u0627\u0644\u0641\u062d\u0635 ( UV-vis \u2013 X.R.D<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">FE-SEM- FTIR \u2013 AFM)<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">6- \u00a0\u062f\u0631\u0627\u0633\u0629 \u0641\u0639\u0627\u0644\u064a\u0629 \u062a\u062b\u0628\u064a\u0637 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0644\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0644\u0623\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">7- \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0633\u0627\u0645 \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0644\u062c\u0633\u064a\u0645\u0627\u062a \u0627\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0636\u062f \u062e\u0637\u0648\u0637 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0628\u0634\u0631\u064a\u0629 ( \u062e\u0644\u0627\u064a\u0627 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064aMCF-7\u00a0\u00a0 \u0648\u062e\u0644\u0627\u064a\u0627 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u0628\u0631\u0648\u0633\u062a\u0627\u062a PC3 \u00a0\u0628\u0637\u0631\u064a\u0642\u0629 \u0641\u062d\u0635 \u0627\u0644\u0633\u0645\u064a\u0629) \u00a0( MTT assay<\/span><\/h4>\n<h4 style=\"text-align: justify\"><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">\u0641\u064a \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u060c \u062a\u0645 \u0627\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 71 \u0639\u0632\u0644\u0629 \u0645\u0646 \u0627\u0644\u0643\u0644\u0628\u0633\u064a\u0644\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0645\u0646 144 \u0639\u064a\u0646\u0629 \u0633\u0631\u064a\u0631\u064a\u0629 \u062a\u0645 \u062c\u0645\u0639\u0647\u0627 \u0645\u0646 \u0641\u0628\u0631\u0627\u064a\u0631 \u0627\u0644\u0649 \u0623\u0628\u0631\u064a\u0644 \u0644\u0633\u0646\u0629 2019\u00a0 \u0645\u0646 \u0633\u062a\u0629 \u0645\u0633\u062a\u0634\u0641\u064a\u0627\u062a \u0641\u064a \u0628\u063a\u062f\u0627\u062f.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">\u062a\u0645 \u062a\u0634\u062e\u064a\u0635 \u0639\u0632\u0644\u0627\u062a \u0643\u0644\u064a\u0628\u0633\u064a\u0644\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0627\u0633\u062a\u0632\u0631\u0627\u0639 \u0639\u0644\u0649 \u0627\u0644\u0627\u0648\u0633\u0627\u0637 \u0627\u0644\u0632\u0631\u0639\u064a\u0629 \u0627\u0644\u0627\u0646\u062a\u0642\u0627\u0626\u064a\u0629 \u060c \u0634\u0643\u0644 \u0627\u0644\u0645\u0633\u062a\u0639\u0645\u0631\u0627\u062a \u060c \u0635\u0628\u063a\u0629 \u062c\u0631\u0627\u0645 \u060c \u0627\u0644\u062e\u0635\u0627\u0626\u0635 \u0627\u0644\u0645\u062c\u0647\u0631\u064a\u0629. \u0648\u0623\u062e\u064a\u0631\u0627 \u0646\u0638\u0627\u0645 \u0641\u0627\u064a\u062a\u0643 -2 \u0627\u0644\u0645\u062f\u0645\u062c Vitek-2 compact system . \u0628\u0644\u063a \u0639\u062f\u062f \u0648\u0646\u0633\u0628\u0629 \u0639\u0632\u0644\u0627\u062a \u0643\u0644\u064a\u0628\u0633\u064a\u0644\u0627 42 \u0639\u0632\u0644\u0629 (59\u066a) \u0645\u0646 \u0627\u0644\u062a\u0647\u0627\u0628 \u0627\u0644\u0645\u0633\u0627\u0644\u0643 \u0627\u0644\u0628\u0648\u0644\u064a\u0629 \u0648 17 \u0639\u0632\u0644\u0629 (24\u066a) \u0645\u0646 \u0627\u0644\u062f\u0645 \u0648 4 \u0639\u0632\u0644\u0627\u062a (6\u066a) \u0645\u0646 \u0627\u0644\u0628\u0644\u063a\u0645 \u0648 3\u0639\u0632\u0644\u0627\u062a (4\u066a) \u0645\u0646 \u0645\u0631\u0636\u0649 \u0627\u0644\u062d\u0631\u0648\u0642 \u0648 3 \u0639\u0632\u0644\u0627\u062a ( 4 \u066a) \u0645\u0646 \u0627\u0644\u0642\u064a\u062d \u0648 \u0639\u0632\u0644\u062a\u0627\u0646 (2 \u066a) \u0645\u0646 \u0639\u062f\u0648\u0649 \u0627\u0644\u062c\u0631\u0648\u062d.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">\u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u062e\u062a\u0628\u0627\u0631\u0627\u062a \u0627\u0644\u062d\u0633\u0627\u0633\u064a\u0629 \u0644\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0623\u0646 \u0623\u0639\u0644\u0649 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u0643\u0644\u0628\u0633\u064a\u0644\u0627\u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0643\u0627\u0646\u062a \u0636\u062f \u0627\u0644\u0633\u064a\u0641\u0648\u062a\u0627\u0643\u0633\u064a\u0645 52 (73 \u066a) \u060c \u0648\u0623\u0642\u0644 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u0643\u0644\u0628\u0633\u064a\u0644\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0643\u0627\u0646\u062a \u0636\u062f \u0627\u0644\u0644\u064a\u0641\u0648\u0641\u0644\u0648\u0643\u0633\u0627\u0633\u064a\u0646 63 (88 \u066a). \u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c 41 (\u066a) \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u0633\u064a\u0641\u062a\u0627\u0632\u064a\u062f\u064a\u0645 \u060c 36 (\u066a) \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u062a\u0648\u0628\u0631\u0627\u0645\u064a\u0633\u064a\u0646 \u060c 33 (\u066a) \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u0628\u064a\u0628\u064a\u0631\u0627\u0633\u064a\u0644\u064a\u0646 \u060c 30 (\u066a) \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u0623\u0632\u064a\u062a\u0631\u064a\u0648\u0646\u0627\u0645 \u060c 28 (\u066a) \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644 \u062c\u064a\u0646\u062a\u0627\u0645\u0627\u064a\u0633\u064a\u0646 \u060c 12 (\u066a) \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u0633\u064a\u0641\u062a\u0631\u064a\u0627\u0643\u0633\u0648\u0646 \u060c 6 (\u066a) \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u0633\u064a\u0628\u0631\u0648\u0641\u0644\u0648\u0643\u0633\u0627\u0633\u064a\u0646 \u0648 5 (\u066a) \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u0644\u064a\u0641\u0648\u0641\u0644\u0648\u0643\u0633\u0627\u0633\u064a\u0646. \u0628\u0627\u0644\u0625\u0636\u0627\u0641\u0629 \u0625\u0644\u0649 \u0630\u0644\u0643 \u060c \u0643\u0634\u0641\u062a \u0628\u0639\u0636 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0639\u0646 \u0648\u062c\u0648\u062f \u0645\u0642\u0627\u0648\u0645\u0629 \u0645\u062a\u0648\u0633\u0637\u0629 \u0636\u062f \u0628\u0639\u0636 \u0627\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">\u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u0647\u064a \u0627\u0644\u0623\u0648\u0644\u0649 \u0627\u0644\u062a\u064a \u064a\u062a\u0645 \u0641\u064a\u0647\u0627 \u062a\u0635\u0646\u064a\u0639 \u062c\u0633\u064a\u0645\u0627\u062a \u0623\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0639\u0646 \u0637\u0631\u064a\u0642 \u062e\u0644\u0637 \u0631\u0627\u0634\u062d \u0645\u0632\u0631\u0639\u0629 \u0627\u0644\u0643\u0644\u0628\u0633\u064a\u0644\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0645\u0639 \u0645\u062d\u0644\u0648\u0644 \u0646\u062a\u0631\u0627\u062a \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u060c \u064a\u062a\u063a\u064a\u0631 \u0644\u0648\u0646 \u0627\u0644\u062e\u0644\u064a\u0637 \u0645\u0646 \u0627\u0644\u0623\u0635\u0641\u0631 \u0627\u0644\u0641\u0627\u062a\u062d \u0625\u0644\u0649 \u0627\u0644\u0623\u0628\u064a\u0636 \u060c \u0645\u0645\u0627 \u064a\u0634\u064a\u0631 \u0625\u0644\u0649 \u062a\u0643\u0648\u0646 \u062c\u0632\u064a\u0626\u0627\u062a \u0623\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 MgONPs.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">\u062f\u0631\u0633\u062a \u062e\u0635\u0627\u0626\u0635 \u062c\u0632\u064a\u0626\u0627\u062a \u0623\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 MgONPs \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0645\u0637\u064a\u0627\u0641 \u0627\u0644\u0627\u0634\u0639\u0629 \u0641\u0648\u0642 \u0627\u0644\u0628\u0646\u0641\u0633\u062c\u064a\u0629 \u0627\u0644\u0645\u0631\u0626\u064a\u0629 UV-visible spectroscopy \u060c \u0645\u0637\u064a\u0627\u0641 \u0645\u062a\u062d\u0648\u0644 \u0641\u0648\u0631\u0628\u064a\u0647 \u0644\u0644\u0627\u0634\u0639\u0629 \u062a\u062d\u062a \u0627\u0644\u062d\u0645\u0631\u0627\u0621 FTIR spectroscopy \u060c \u062a\u062d\u0644\u064a\u0644 \u062d\u064a\u0648\u062f \u0627\u0644\u0623\u0634\u0639\u0629 \u0627\u0644\u0633\u064a\u0646\u064a\u0629 X-Ray Diffraction Analysis \u060c \u0627\u0644\u0645\u062c\u0647\u0631 \u0627\u0644\u0627\u0644\u0643\u062a\u0631\u0648\u0646\u064a \u0627\u0644\u0645\u0627\u0633\u062d \u0644\u0645\u062c\u0627\u0644 \u0627\u0644\u0627\u0646\u0628\u0639\u0627\u062b FESEM \u060c \u0648 \u0645\u062c\u0647\u0631 \u0627\u0644\u0642\u0648\u0629 \u0627\u0644\u0630\u0631\u064a\u0629 AMF. \u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0637\u064a\u0641\u064a \u0644\u0644\u0623\u0634\u0639\u0629 \u0641\u0648\u0642 \u0627\u0644\u0628\u0646\u0641\u0633\u062c\u064a\u0629 \u0627\u0644\u0645\u0631\u0626\u064a\u0629 \u0623\u0646 \u0630\u0631\u0648\u0629 \u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635 \u0628\u0644\u063a\u062a \u0642\u064a\u0645\u062a\u0647\u0627 )250( \u0646\u0627\u0646\u0648\u0645\u062a\u0631 \u0645\u062b\u0628\u062a\u0647 \u0648\u062c\u0648\u062f MgONPs. \u0623\u0638\u0647\u0631 \u0645\u0637\u064a\u0627\u0641 FTIR \u0627\u0644\u062d\u0632\u0645 \u0641\u064a \u0627\u0644\u0642\u0645\u0645 3369 \u060c 2930 \u060c 1629 \u060c 1367 \u0648 434 \u0633\u0645 -1. \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u062a\u064a \u0644\u0648\u062d\u0638\u062a \u0641\u064a 434 \u0633\u0645 \u00af1 \u062a\u0645\u062b\u0644 \u062a\u0634\u0643\u064a\u0644 \u062c\u0633\u064a\u0645\u0627\u062a \u0623\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 MgONPs. \u0648\u0642\u062f \u0644\u0648\u062d\u0638\u062a \u0642\u0645\u0645 XRD \u0641\u064a \u0627\u0644\u0642\u064a\u0645 (111) \u060c (200) \u060c (220) \u060c (311) \u060c (222). \u062a\u0624\u0643\u062f \u0647\u0630\u0647 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0623\u0646 \u0627\u0644\u0645\u0648\u0627\u062f \u0627\u0644\u062a\u064a \u062a\u0645 \u0627\u062e\u062a\u0628\u0627\u0631\u0647\u0627 \u0639\u0628\u0627\u0631\u0629 \u0639\u0646 \u062c\u0633\u064a\u0645\u0627\u062a \u0623\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 MgONPs \u0648\u0630\u0627\u062a \u062f\u0631\u062c\u0629 \u0646\u0642\u0627\u0648\u0629 \u0639\u0627\u0644\u064a\u0629. \u0635\u0648\u0631\u0629 FESEM \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0640 MgONPs \u060c \u0648\u0627\u0644\u062a\u064a \u062a\u0638\u0647\u0631 \u0628\u0646\u064a\u0629 \u062a\u0634\u0628\u0647 \u0627\u0644\u0631\u0642\u0627\u0642\u0627\u062a \u0628\u0633\u0628\u0628 \u062a\u062c\u0645\u064a\u0639 \u0639\u062f\u0629 \u0622\u0644\u0627\u0641 \u0645\u0646 MgONPs. \u0648\u0642\u062f \u0644\u0648\u062d\u0638 \u062d\u062c\u0645 MgONPs \u0645\u0646 \u0642\u064a\u0627\u0633\u0627\u062a \u062a\u0635\u062d\u064a\u062d \u0627\u0644\u0642\u0645\u0629 \u0644\u0645\u062c\u0647\u0631 \u0627\u0644\u0642\u0648\u0629 \u0627\u0644\u0630\u0631\u064a\u0629 \u00a0AFM \u0648\u062a\u0645 \u062a\u062d\u062f\u064a\u062f \u0634\u0643\u0644 MgONPs.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">\u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0646\u0634\u0627\u0637 \u0627\u0644\u0645\u0636\u0627\u062f \u0644\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0644\u062c\u0633\u064a\u0645\u0627\u062a \u0623\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 MgONPs \u0639\u0646\u062f 100 \u0648 200 \u0648 400 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645 \/ \u0645\u0644 \u0643\u0627\u0646\u062a \u0646\u0634\u0637\u0629 \u0636\u062f \u0627\u0644\u062c\u0631\u0627\u0645 \u0633\u0627\u0644\u0628\u0629 \u0648\u0625\u064a\u062c\u0627\u0628\u064a\u0629 \u0627\u0644\u062c\u0631\u0627\u0645. \u0648\u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0623\u0646 MgONPs \u0644\u0647\u0627 \u0646\u0634\u0627\u0637 \u0645\u0636\u0627\u062f \u0644\u0644\u0627\u063a\u0634\u064a\u0629 \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0636\u062f \u0623\u0631\u0628\u0639\u0629 \u0623\u0646\u0648\u0627\u0639\u060c \u0627\u062b\u0646\u0627\u0646 \u062a\u0646\u062a\u0645\u064a \u0625\u0644\u0649 \u0627\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u0627\u0644\u0633\u0627\u0644\u0628\u0629 \u062c\u0631\u0627\u0645 \u0648\u0623\u062b\u0646\u0627\u0646 \u062a\u0646\u062a\u0645\u064a \u0625\u0644\u0649 \u0627\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u0627\u0644\u0627\u064a\u062c\u0627\u0628\u064a\u0629 \u062c\u0631\u0627\u0645 \u0628\u062a\u0631\u0643\u064a\u0632 200 \u0648 400 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645 \/ \u0645\u0644.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">\u0623\u0634\u0627\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0625\u0644\u0649 \u0623\u0646 MgONPs \u062a\u0633\u0628\u0628\u062a \u0641\u064a \u0627\u0646\u062e\u0641\u0627\u0636 \u0641\u064a \u062d\u064a\u0648\u064a\u0629 \u0627\u0644\u062e\u0644\u064a\u0629 \u0628\u0637\u0631\u064a\u0642\u0629 \u062a\u0639\u062a\u0645\u062f \u0639\u0644\u0649 \u0627\u0644\u062c\u0631\u0639\u0629 \u0639\u0644\u0649 \u0643\u0644 \u0645\u0646 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 MCF-7 \u0648 PC3 \u060c \u0645\u0639 \u062d\u0633\u0627\u0628 IC50 \u0645\u0646 76.87 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645 \/ \u0645\u0644 \u0648 136.0 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645 \/ \u0645\u0644 \u060c \u0639\u0644\u0649 \u0627\u0644\u062a\u0648\u0627\u0644\u064a. \u0646\u0633\u062a\u0646\u062a\u062c \u0627\u0646 \u0627\u0644\u062a\u062e\u0644\u064a\u0642 \u0627\u0644\u062d\u064a\u0648\u064a \u0644\u062c\u0632\u064a\u0626\u0627\u062a \u0623\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 MgONPs \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0631\u0627\u0634\u062d \u0645\u0632\u0631\u0639\u0629 \u0627\u0644\u0643\u0644\u0628\u0633\u064a\u0644\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0644\u0647 \u0646\u0634\u0627\u0637 \u0645\u0636\u0627\u062f \u0644\u0644\u0645\u064a\u0643\u0631\u0648\u0628\u0627\u062a \u060c \u0645\u0636\u0627\u062f \u0644\u0644\u0623\u063a\u0634\u064a\u0629 \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0648\u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u0633\u0631\u0637\u0627\u0646 \u0648\u064a\u0645\u0643\u0646 \u0627\u0633\u062a\u062e\u062f\u0627\u0645\u0647 \u0643\u0639\u0627\u0645\u0644 \u0639\u0644\u0627\u062c\u064a.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">\u0648\u062e\u0644\u0635 \u0627\u0644\u0637\u0627\u0644\u0628 \u0627\u0644\u0649 \u0627\u0644\u062a\u0648\u0635\u064a\u0627\u062a \u0627\u0644\u0627\u062a\u064a\u0629 :<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">1- \u0639\u0632\u0644 \u0648\u062a\u0634\u062e\u064a\u0635 \u0627\u0644\u0645\u0633\u062a\u062e\u0644\u0635 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a \u0648\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0645\u0648\u0627\u062f \u0627\u0644\u0641\u0639\u0627\u0644\u0629 (\u0625\u0646\u0632\u064a\u0645\u0627\u062a \u060c \u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u060c \u0625\u0644\u062e) \u0641\u064a \u062a\u062d\u0636\u064a\u0631 \u062c\u0632\u064a\u0626\u0627\u062a \u0623\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 ( MgONPs ) \u00a0<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">2- \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u062a\u0622\u0632\u0631\u064a \u0644\u062c\u0632\u0626\u064a\u0627\u062a \u0627\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 ( MgONPs ) \u00a0\u0648\u0627\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0644\u062a\u0639\u0632\u064a\u0632 \u0627\u0644\u0642\u062f\u0631\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">3- &#8211; \u00a0\u062f\u0631\u0627\u0633\u0629 \u0641\u0639\u0627\u0644\u064a\u0629 \u062a\u062b\u0628\u064a\u0637 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u062c\u0632\u0626\u064a\u0627\u062a \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0644\u0623\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0627\u0644\u0645\u0633\u062a\u0648\u0649 \u0627\u0644\u062c\u064a\u0646\u064a.<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">4- \u062f\u0631\u0627\u0633\u0629 \u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u062c\u0632\u0626\u064a\u0627\u062a \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0644\u0623\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 ( MgONPs ) \u0639\u0644\u0649 \u0639\u0648\u0627\u0645\u0644 \u0638\u0631\u0627\u0648\u0629 \u0627\u062e\u0631\u0649 \u0645\u062b\u0644 ( \u0645\u0636\u062e\u0627\u062a \u0627\u0644\u062f\u0641\u0642 , \u0627\u0646\u062a\u0627\u062c \u0627\u0644\u0647\u064a\u0645\u0648\u0644\u0627\u064a\u0633\u0646 )<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">5- \u062f\u0631\u0627\u0633\u0629 \u00a0\u0627\u0644\u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u0633\u0631\u0637\u0627\u0646 \u0644\u062c\u0632\u0626\u064a\u0627\u062a \u0627\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 ( MgONPs ) \u0639\u0644\u0649 \u062e\u0637\u0648\u0637 \u0627\u062e\u0631\u0649 \u0645\u0646 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0633\u0631\u0637\u0627\u0646\u064a\u0629 \u0627\u0644\u0628\u0634\u0631\u064a\u0629<\/span><\/h4>\n<h4 style=\"text-align: justify\"><span style=\"color: #000080\">6- \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0646\u0634\u0627\u0637 \u0627\u0644\u0633\u0627\u0645 \u0644\u062c\u0632\u0626\u064a\u0627\u062a \u0627\u0648\u0643\u0633\u064a\u062f \u0627\u0644\u0645\u063a\u0646\u064a\u0633\u064a\u0648\u0645 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 ( MgONPs ) \u0641\u064a \u0627\u0644\u062c\u0633\u0645 \u0627\u0644\u062d\u064a<\/span><\/h4>\n<hr \/>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">BIOSYNTHYSIS OF MAGNESIUN OXIDE NANOPARTICALS FROM KLEBSIELLA PNEUMONIA LOCALLY ISOLATED<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">By Hussein.H.Mudhafar<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">Supervised by Asst.Prof. Dr. Esam j. Al-kalifawi<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Objectives of the study<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">The present study aimed to studying the effects of magnesium Oxide Nanoparticles as antibacterial, anti-biofilm and anti-cancer agent \u00a0such objective required the following procedure<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">. Isolation and identification of klebsiella pneumonia from different clinical samples and using traditional methods (culture on selective media, biochemical test and vitek-2 system<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">\u00a0Determining the antibiotic susceptibility of klebsiella pneumonia isolates against multiple classes of antibiotic<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">. Determining the prevalence of biofilm formations for gram negative bacteria (klebsiella. Pneumoniae andPseudomonas aeruginosa) and Gram positive bacteria (Oxacillin resistance Staphylococcus aureus (ORSA) and Vancomycin resistance Staphylococcus aureus (VRSA) among the isolates using microtiter plates method<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">. Biosynthesis of magnesium oxide nanoparticles using k. pneumonia extract<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">Characterization of magnesium oxide nanoparticles using different techniques (UV-Vis, X.R.D, FE-SEM, AFM and FTIR)<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">. Studying the inhibition effectiveness of bacterial biofilm isolates using magnesium oxide nanoparticle<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">. Investigating the cytotoxic effect of magnesium oxide nanoparticles against human cell lines (MCF-7 and PC3) of oxide nanoparticles using MTT assay<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">Abstract<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">In the present study, 71 isolates of Klebsiellapneumoniae were obtained from 144 different clinical samples which are collected from February to April, 2019from six hospitals in Baghdad<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">Klebsiella pneumoniae isolates were diagnosed using selective media, colonial morphology, Gram staining and microscopic characteristics. Finally, diagnosed by Vitek-2 Compact system.The number and percentage of K.pneumoniaeisolates were 42(59%) isolates from urinary tract infection, 17(24%) isolates from blood, 4(6%) isolates from sputum, 3(4%) isolates from burn patients, 3(4%) isolates from pus and 2(3%) isolates from wounds infection<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">The Results of antibiotic sensitivity tests showed that the highest resistance of K. pneumoniae was against cefotaxim (52%), and the lowest resistance of K. pneumoniae was against levofloxacin (5%). The results showed 41 (%) resistant for ceftazidim, 36 (%) resistant for tobramycin, 33 (%) resistant for piperacilin, 30 (%) resistant for azetreonam, 28(%) resistant for gentamycin, 12 (%) resistant for ceftriaxone, 6 (%) resistant for cibrofloxacine and 5 (%) resistant for levofloxacin<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">The current study is the first study in which the magnesium oxide nanoparticles were synthesized by using K. pneumoniae culture filtrate with magnesium nitrate solution, the color of the mixture changed from pale yellow to white, indicating the formation of MgONPs<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">The MgONPs were characterized using UV-Visible analysis (UV-Vis),X-ray diffraction (XRD) analysis, Fourier Transform Infrared (FTIR) Spectroscopy Analysis, Field Emission Scanning Electron Microscope (FE-SEM), and Atomic Force Microscopy (AFM)<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">The results of UV-Vis spectroscopy showed that the absorption peak was at 250 nm proved the existence of MgONPs<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">The FTIR spectra showed bands at 3369, 2930, 1629, 1367and 434 cm. The peak observed at 434 cm represents the formation of MgONPs. The XRD peaks were observed at values (111), (200), (220), (311), and (222). These results confirm that the material tested are MgONPs with high purity<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">The FESEM image of MgONPs, which exhibit flakes-like structure due to the aggregation of several thousand of MgONPs. The size of MgONPs was in the range of 35\u201395 nm from tip-corrected AFM measurements and the shape of MgONPs were determined<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">The results of antibacterial activity showed that MgONPs at concentration of (100, 200 and 400) \u00b5g\/ml were active against Gram negative and Gram positive bacteria<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">The results showed that MgONPs have antibiofilm activity against four types of bacteria, two belong to Gram negative bacteria and two for Gram positive bacteria at (200) and (400) \u00b5g\/mL concentrations<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">The results indicated that MgONPs caused a reduction in cell viability in a dose-dependent manner on both MCF-7and PC3 cell lines, with a calculating IC50 of 76.87 \u00b5g\/mL and 136.0 \u00b5g\/mL respectively. It was concluded that biosynthesis of MgONPs using Klebsiella pneumoniae culture filtrate which have antibacterial, antibiofilm and anticancer activity and it can be used as therapeutic agent<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">RECOMMENDATION<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">Isolation and diagnosis of the bacterial extract and the use of active substances (enzymes , proteins, etc.) in the preparation of magnesium oxidenanoparticles<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">Study the synergistic effect of MgONPs and antibiotics to enhance antibacterial potential<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">Study the antibiofilm activity of MgONPs on genetic level<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">Study anti-virulence activity of MgONPs on other virulence factors such as swarming, hemolysin production and efflux pumps<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">Study the anticancer activity of MgONPs on other lines of cancer cells<\/span><\/h3>\n<h3 style=\"text-align: left\"><span style=\"color: #993366\">Study the cytotoxic activity of MgONPs in vivo<\/span><\/h3>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u0646\u0627\u0642\u0634 \u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u062a\u0631\u0628\u064a\u0629 \u0644\u0644\u0639\u0644\u0648\u0645 \u0627\u0644\u0635\u0631\u0641\u0629 ( \u0627\u0628\u0646 \u0627\u0644\u0647\u064a\u062b\u0645 ) \u0631\u0633\u0627\u0644\u0629 \u0627\u0644\u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u0627\u0644\u0645\u0648\u0633\u0648\u0645\u0629 ( \u00a0\u0627\u0644\u062a\u062e\u0644\u064a\u0642 \u0627\u0644\u062d\u064a\u0648\u064a \u0644\u062c\u0632\u064a\u0626\u0627\u062a&#8230;.<a class=\"cz_readmore\" href=\"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=32853\"><i class=\"fa fa-angle-left\" aria-hidden=\"true\"><\/i><span>\u0627\u0644\u0645\u0632\u064a\u062f<\/span><\/a><\/p>\n","protected":false},"author":25,"featured_media":32855,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_lmt_disableupdate":"no","_lmt_disable":"","footnotes":""},"categories":[7,16],"tags":[23,48],"class_list":["post-32853","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-7","category-16","tag-ihcoedu","tag-bio"],"modified_by":"ihcoedu","_links":{"self":[{"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/32853","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/users\/25"}],"replies":[{"embeddable":true,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32853"}],"version-history":[{"count":0,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/32853\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/media\/32855"}],"wp:attachment":[{"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32853"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32853"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32853"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}