{"id":331,"date":"2014-02-06T11:04:41","date_gmt":"2014-02-06T08:04:41","guid":{"rendered":""},"modified":"2014-02-06T11:04:41","modified_gmt":"2014-02-06T08:04:41","slug":"","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=331","title":{"rendered":"\u0645\u062d\u0627\u0636\u0631\u0629 \u0628\u0639\u0646\u0648\u0627\u0646 ( \u0641\u0631\u0637 \u0627\u0644\u062a\u0623\u0643\u0633\u062c : \u062d\u064a\u0627\u0629 \u0627\u0648 \u0645\u0648\u062a \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 )"},"content":{"rendered":"
\n


 <\/span><\/p>\n

\u0636\u0645\u0646 \u0627\u0644\u0645\u0648\u0633\u0645 \u0627\u0644\u062b\u0642\u0627\u0641\u064a  \u0644\u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u062a\u0631\u0628\u064a\u0629 \u0644\u0644\u0639\u0644\u0648\u0645 \u0627\u0644\u0635\u0631\u0641\u0629\/\u0625\u0628\u0646 \u0627\u0644\u0647\u064a\u062b\u0645 \u0623\u0642\u064a\u0645\u062a \u0639\u0644\u0649 \u0642\u0627\u0639\u0629 \u0627\u0644\u0633\u064a\u0645\u0646\u0627\u0631\u060c \u0645\u062d\u0627\u0636\u0631\u0629 \u0628\u0639\u0646\u0648\u0627\u0646 ((  \u0641\u0631\u0637 \u0627\u0644\u062a\u0623\u0643\u0633\u062c : \u062d\u064a\u0627\u0629 \u0627\u0648 \u0645\u0648\u062a \u0627\u0644\u062e\u0644\u0627\u064a\u0627\u0627\u0644\u0631\u0626\u0648\u064a\u0629  ) \u0642\u0627\u0645 \u0628\u0625\u0644\u0642\u0627\u0621\u0647\u0627 <\/span>\u0645 . \u062f . \u0647\u0646\u0627\u062f\u064a \u0633\u0627\u0644\u0645 \u0639\u0628\u062f \u0627\u0644\u0635\u0627\u062d\u0628 \u0627\u0644\u0634\u0645\u062e\u0627\u0646\u064a<\/span><\/strong> .<\/span> <\/p>\n

\u0648\u0642\u062f \u0628\u064a\u0646 \u0627\u0644\u0645\u062d\u0627\u0636\u0631 \u0627\u0646 \u0641\u0631\u0637 \u0627\u0644\u062a\u0623\u0643\u0633\u062c  \u064a\u0633\u0627\u0647\u0645 \u0641\u064a \u0627\u0644\u062d\u0641\u0627\u0638 \u0639\u0644\u0649 \u062d\u064a\u0627\u0629 \u0627\u0644\u0645\u0631\u0636\u0649 \u0627\u0644\u0644\u0630\u064a\u0646  \u064a\u0639\u0627\u0646\u0648\u0646  \u0648\u0645\u0646 \u0641\u0634\u0644 \u0627\u0648 \u062a\u0648\u0642\u0641 \u0627\u0644\u062a\u0646\u0641\u0633 , \u0648\u0628\u0627\u0644\u0631\u063a\u0645 \u0645\u0646 \u0630\u0644\u0643 \u0641\u0623\u0646\u0647 \u064a\u0624\u062f\u064a \u0627\u0644\u0649 \u0639\u0648\u0627\u0642\u0628 \u0645\u0631\u0636\u064a\u0629 \u0645\u0646\u0647\u0627 : \u0627\u0644\u0627\u0644\u062a\u0647\u0627\u0628 \u0627\u0644\u0646\u0627\u062c\u0645 \u0639\u0646 \u062a\u0644\u0641 \u0627\u0644\u0627\u0646\u0633\u062c\u0629 \u0627\u0644\u062a\u0623\u0643\u0633\u062f\u064a , \u062a\u0644\u064a\u0641 \u0631\u0626\u0648\u064a, \u0648 \u0630\u0645\u0629 \u0627\u0644\u0631\u0626\u0629 , \u0636\u0639\u0641 \u0627\u0644\u0627\u0647\u062f\u0627\u0628 \u0648 \u0645\u0648\u062a \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0639\u0646\u062f \u0627\u0644\u062a\u0636\u0631\u0631 \u0627\u0644\u062d\u0627\u062f \u0627\u0648 \u0627\u0644\u0645\u0632\u0645\u0646 \u0644\u0644\u0631\u0626\u0629 .<\/span>
<\/span><\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

<\/span><\/div>\n

 \u0627\u0646 \u0627\u0644\u0622\u0644\u064a\u0627\u062a \u0627\u0644\u0645\u0624\u062f\u064a\u0629  \u0627\u0644\u0649 \u062d\u062f\u0648\u062b \u0647\u0630\u0647 \u0627\u0644\u0627\u0636\u0631\u0627\u0631 \u0644\u0627\u062a\u0632\u0627\u0644 \u063a\u064a\u0631 \u0645\u0641\u0647\u0648\u0645\u0629 \u0648\u0647\u064a \u0642\u064a\u062f \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0648 \u0645\u0646\u0647\u0627 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0645\u0648\u0636\u062d\u0629 \u0641\u064a \u0647\u0630\u0647 \u0627\u0644\u0645\u062d\u0627\u0636\u0631\u0629 , \u062d\u064a\u062b \u062a\u0645  \u062a\u062d\u062f\u064a\u062f \u0645\u0648\u0642\u0639 \u0648\u0622\u0644\u064a\u0629 \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0648\u0627\u0644\u0627\u0636\u0631\u0627\u0631 \u0627\u0644\u0646\u0627\u062c\u0645\u0629 \u0639\u0646 \u0641\u0631\u0637 \u0627\u0644\u062a\u0623\u0643\u0633\u062c \u0645\u0646 \u062e\u0644\u0627\u0644 \u062f\u0631\u0627\u0633\u0629   \u0627\u0644\u0622\u062a\u064a  : <\/span> <\/p>\n

<\/span><\/p>\n

– Tight  junction protein detection<\/span><\/p>\n

. <\/span>– Pro –inflammatory  cytokines  detection<\/span><\/p>\n

.<\/span>– Measurement  of  transepithelial  resistance ( TER)<\/span><\/p>\n

– Immunofluorescence  detection  of   ZO-1,polyclonal  rabbit ZO-2 , confocal microscopy, Neuron J tracing too<\/span> <\/p>\n

.<\/span>– Detection  of cytokines in the medium by  ELISA<\/span><\/p>\n

.<\/span> <\/span>– Detection  of  cytokines expression  by RT- qPCR<\/span><\/p>\n

 . <\/span>– Cytokines of interest IL-6, IL-8 and TNF-α<\/span><\/p>\n

– Detection of ZO-1 expression by RT-PCR.<\/span><\/p>\n

<\/span><\/div>\n

\n

\n

\u0648\u0642\u062f \u0628\u064a\u0646\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0627\u062a\u064a  : <\/span><\/p>\n

1. \u0642\u062f \u0644\u0627\u064a\u0624\u062f\u064a \u0641\u0631\u0637 \u0627\u0644\u062a\u0623\u0643\u0633\u062c \u0627\u0644\u0649 \u0645\u0648\u062a \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0627\u062d\u064a\u0627\u0646\u0627 , \u0628\u0644 \u062a\u0644\u0641 \u0641\u064a \u0627\u0644\u0631\u0648\u0627\u0628\u0637 \u0627\u0644\u062e\u0644\u0648\u064a\u0629 \u0645\u0645\u0627 \u064a\u0624\u062f\u064a \u0627\u0644\u0649 \u0627\u0646\u0633\u0644\u0627\u062e \u0627\u0648 \u0627\u0646\u0641\u0635\u0627\u0644 \u0647\u0630\u0647 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 .<\/span><\/p>\n

2. \u0627\u0646 \u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u0627\u0643\u0633\u062f\u0629 \u0645\u062b\u0644 \u0641\u064a\u062a\u0627\u0645\u064a\u0646\u0627\u062a  <\/span>C , E )<\/span> ) \u062a\u0639\u0645\u0644 \u0639\u0644\u0649 \u062a\u0648\u0641\u064a\u0631 \u062d\u0645\u0627\u064a\u0629 \u062c\u0632\u0626\u064a\u0629 \u0644\u062d\u0627\u0644\u0629 \u0641\u0642\u062f\u0627\u0646 \u0627\u0644\u0627\u0647\u062f\u0627\u0628 \u0627\u0644\u0646\u0627\u062c\u0645 \u0639\u0646 \u0641\u0631\u0637 \u0627\u0644\u062a\u0623\u0643\u0633\u062c .<\/span><\/p>\n

3. \u0627\u0646\u062e\u0641\u0627\u0636 \u0645\u0633\u062a\u0648\u0649 \u0628\u0631\u0648\u062a\u064a\u0646 <\/span>ZO – 1 <\/span>   \u0644\u0644\u0631\u0648\u0627\u0628\u0637 \u0627\u0644\u0645\u062d\u0643\u0645\u0629 \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 \u0648 \u0627\u0644\u0645\u0631\u062a\u0628\u0637 \u0645\u0639 \u0627\u0631\u062a\u0641\u0627\u0639 \u0646\u0633\u0628\u0629 \u0627\u0644\u0633\u0627\u064a\u062a\u0648\u0643\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0624\u062f\u064a\u0629 \u0644\u0644\u0627\u062a\u0647\u0627\u0628\u0627\u062a . <\/span><\/p>\n

4. <\/span>\u0627\u0646 \u0627\u0644\u0641\u064a\u062a\u0627\u0645\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0636\u0627\u062f\u0629\u0644\u0644\u0627\u0643\u0633\u062f\u0629 \u0644\u0639\u0628\u062a \u062f\u0648\u0631\u0627 \u0648\u0642\u0627\u0626\u064a\u0627 \u0641\u064a \u0627\u0644\u062d\u0641\u0627\u0638 \u0639\u0644\u0649 \u0633\u0644\u0627\u0645\u0629 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 .<\/span>
<\/span><\/p>\n

<\/span><\/p>\n

<\/p>\n

\"\"
<\/span><\/div>\n

\n

\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u0636\u0645\u0646 \u0627\u0644\u0645\u0648\u0633\u0645 \u0627\u0644\u062b\u0642\u0627\u0641\u064a \u0644\u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u062a\u0631\u0628\u064a\u0629\u0644\u0644\u0639\u0644\u0648\u0645 \u0627\u0644\u0635\u0631\u0641\u0629\/\u0625\u0628\u0646 \u0627\u0644\u0647\u064a\u062b\u0645 \u0623\u0642\u064a\u0645\u062a \u0639\u0644\u0649 \u0642\u0627\u0639\u0629 \u0627\u0644\u0633\u064a\u0645\u0646\u0627\u0631\u060c \u0645\u062d\u0627\u0636\u0631\u0629 \u0628\u0639\u0646\u0648\u0627\u0646 (( \u0641\u0631\u0637 \u0627\u0644\u062a\u0623\u0643\u0633\u062c : \u062d\u064a\u0627\u0629 \u0627\u0648 \u0645\u0648\u062a \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0631\u0626\u0648\u064a\u0629 ) \u0642\u0627\u0645 \u0628\u0625\u0644\u0642\u0627\u0621\u0647\u0627 \u0645 . \u062f . \u0647\u0646\u0627\u062f\u064a \u0633\u0627\u0644\u0645 \u0639\u0628\u062f \u0627\u0644\u0635\u0627\u062d\u0628 \u0627\u0644\u0634\u0645\u062e\u0627\u0646\u064a .
\u0648\u0642\u062f \u0628\u064a\u0646 \u0627\u0644\u0645\u062d\u0627\u0636\u0631 \u0627\u0646 \u0641\u0631\u0637 \u0627\u0644\u062a\u0623\u0643\u0633\u062c \u064a\u0633\u0627\u0647\u0645 \u0641\u064a \u0627\u0644\u062d\u0641\u0627\u0638 \u0639\u0644\u0649 \u062d\u064a\u0627\u0629 \u0627\u0644\u0645\u0631\u0636\u0649 \u0627\u0644\u0644\u0630\u064a\u0646 \u064a\u0639\u0627\u0646\u0648\u0646 \u0648\u0645\u0646 \u0641\u0634\u0644 \u0627\u0648 \u062a\u0648\u0642\u0641 \u0627\u0644\u062a\u0646\u0641\u0633 , \u0648\u0628\u0627\u0644\u0631\u063a\u0645 \u0645\u0646 \u0630\u0644\u0643 \u0641\u0623\u0646\u0647 \u064a\u0624\u062f\u064a \u0627\u0644\u0649 \u0639\u0648\u0627\u0642\u0628 \u0645\u0631\u0636\u064a\u0629 \u0645\u0646\u0647\u0627 : \u0627\u0644\u0627\u0644\u062a\u0647\u0627\u0628 \u0627\u0644\u0646\u0627\u062c\u0645 \u0639\u0646 \u062a\u0644\u0641 \u0627\u0644\u0627\u0646\u0633\u062c\u0629 \u0627\u0644\u062a\u0623\u0643\u0633\u062f\u064a ,….<\/i>\u0627\u0644\u0645\u0632\u064a\u062f<\/span><\/a><\/p>\n","protected":false},"author":25,"featured_media":15458,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_lmt_disableupdate":"","_lmt_disable":"","footnotes":""},"categories":[],"tags":[],"class_list":["post-331","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"modified_by":null,"_links":{"self":[{"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/331","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/users\/25"}],"replies":[{"embeddable":true,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=331"}],"version-history":[{"count":0,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/posts\/331\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=\/wp\/v2\/media\/15458"}],"wp:attachment":[{"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=331"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=331"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ihcoedu.uobaghdad.edu.iq\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=331"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}