{"id":33675,"date":"2020-10-28T01:39:07","date_gmt":"2020-10-27T22:39:07","guid":{"rendered":"http:\/\/ihcoedu.uobaghdad.edu.iq\/?p=33675"},"modified":"2020-11-10T10:18:55","modified_gmt":"2020-11-10T07:18:55","slug":"%d9%82%d8%b3%d9%85-%d8%b9%d9%84%d9%88%d9%85-%d8%a7%d9%84%d8%ad%d9%8a%d8%a7%d8%a9-%d9%8a%d9%86%d8%a7%d9%82%d8%b4-%d8%b1%d8%b3%d8%a7%d9%84%d8%a9-%d9%85%d8%a7%d8%ac%d8%b3%d8%aa%d9%8a%d8%b1-%d8%aa-20","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=33675","title":{"rendered":"\u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u064a\u0646\u0627\u0642\u0634 \u0631\u0633\u0627\u0644\u0629 \u0645\u0627\u062c\u0633\u062a\u064a\u0631 \u062a\u062f\u0631\u0633 \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u062e\u0644\u0637\u064a \u0644\u062d\u0627\u0645\u0636 \u0627\u0644\u062e\u0644\u064a\u0643 \u0648\u0645\u0636\u0627\u062f Ceftazidime\u00a0 \u0639\u0644\u0649 \u0646\u0645\u0648 \u0648\u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0641\u064a \u0628\u0643\u062a\u0631\u064a\u0627 Escherichia coli\u00a0 \u0627\u0644\u0645\u0639\u0632\u0648\u0644\u0629 \u0645\u0646 \u062d\u0627\u0644\u0627\u062a \u0633\u0631\u064a\u0631\u064a\u0629 \u0645\u062e\u062a\u0644\u0641\u0629"},"content":{"rendered":"

\"\"<\/span><\/h4>\n

<\/h4>\n

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<\/h4>\n

\u0648\u0647\u062f\u0641\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u0627\u0644\u0649 \u0627\u0644\u062a\u062d\u0631\u064a \u0639\u0646 \u062a\u0623\u062b\u064a\u0631 \u062d\u0627\u0645\u0636 \u0627\u0644\u062e\u0644\u064a\u0643 acetic acid\u00a0\u00a0\u00a0 \u0648\u0645\u0636\u0627\u062f Ceftazidime \u0639\u0644\u0649 \u0646\u0645\u0648 \u0648 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0644\u0628\u0643\u062a\u0631\u064a\u0627 E.coli\u00a0 \u0627\u0644\u0645\u0639\u0632\u0648\u0644\u0629 \u0645\u0646 \u062d\u0627\u0644\u0627\u062a \u0633\u0631\u064a\u0631\u064a\u0629 \u0645\u062e\u062a\u0644\u0641\u0629 .<\/span><\/h4>\n

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\u0632\u0631\u0639\u062a \u0627\u0644\u0645\u0633\u062a\u0639\u0645\u0631\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0627\u0644\u0645\u0646\u0642\u0627\u0629 \u0639\u0644\u0649 \u0627\u0648\u0633\u0627\u0637 MacConkey agar , Eosin Methelen Blue (EMB)\u00a0\u00a0 Blood agar, \u060c \u062b\u0645 \u0634\u062e\u0635\u062a \u0628\u0623\u0633\u062a\u0639\u0645\u0627\u0644 \u0627\u0644\u0627\u062e\u062a\u0628\u0627\u0631\u0627\u062a \u0627\u0644\u0643\u064a\u0645\u0648\u062d\u064a\u0648\u064a\u0629 \u062b\u0645 \u0627\u062c\u0631\u0649 \u0627\u0644\u062a\u0634\u062e\u064a\u0635 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062c\u0647\u0627\u0632 \u0627\u0644\u0641\u0627\u064a\u062a\u0643 . \u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u0634\u062e\u064a\u0635 \u0627\u0646 50 \u0639\u0632\u0644\u0629 \u062a\u0639\u0648\u062f \u0627\u0644\u0649 \u0628\u0643\u062a\u0631\u064a\u0627 E.coli \u0648\u062a\u0636\u0645\u0646\u062a (92%)46 \u0639\u0632\u0644\u0629 \u0645\u0646 \u0627\u0644\u0628\u0648\u0644 \u0648 (8%)4 \u0645\u0646 \u0645\u0633\u062d\u0627\u062a \u0627\u0644\u062c\u0631\u0648\u062d.<\/span><\/h4>\n

\u0627\u062c\u0631\u0649 \u0641\u062d\u0635 \u0627\u0644\u062d\u0633\u0627\u0633\u064a\u0629 \u0644\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0628\u0637\u0631\u064a\u0642\u0629 \u0627\u0646\u062a\u0634\u0627\u0631 \u0627\u0644\u0627\u0642\u0631\u0627\u0635 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 10 \u0645\u0636\u0627\u062f\u0627\u062a \u062d\u064a\u0627\u062a\u064a\u0629 \u060c \u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0646 \u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u0631\u064a\u0627 \u0643\u0627\u0646\u062a 43 \u0639\u0632\u0644\u0629 \u0645\u0646\u0647\u0627 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0645\u0636\u0627\u062f\u064a Trimethoprim\u00a0 \u0648Piperacillin \u0648\u0628\u0646\u0633\u0628\u0629 (%86) \u060c \u0648\u0627\u0646 32 \u0639\u0632\u0644\u0629 \u0645\u0642\u0627\u0648\u0645\u0629\u00a0 \u0644\u0645\u0636\u0627\u062f\u064aCeftazidime \u0648Ciprofloxacin \u0628\u0646\u0633\u0628\u0629 ( %64)\u060c \u0648\u0627\u0646 20 \u0639\u0632\u0644\u0629 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0645\u0636\u0627\u062f Cefoxitin \u0648\u0628\u0646\u0633\u0628\u0629\u00a0\u00a0 40%) ) \u064818 \u0639\u0632\u0644\u0629 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0645\u0636\u0627\u062f Amikacin \u0648\u0628\u0646\u0633\u0628\u0629 (%36) \u0648\u0627\u0646 12 \u0639\u0632\u0644\u0629 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0645\u0636\u0627\u062f\u064aGentamicin \u0648Nitrofurantoin \u0648\u0628\u0646\u0633\u0628\u0629 ( %24) \u060c \u064810 \u0639\u0632\u0644\u0629 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0645\u0636\u0627\u062f Chloramphenicol \u0648\u0628\u0646\u0633\u0628\u0629 (%20) \u060c \u06482 \u0639\u0632\u0644\u0629 \u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0645\u0636\u0627\u062f Imipenem \u0648\u0628\u0646\u0633\u0628\u0629 (%4).<\/span><\/h4>\n

\u062a\u0645 \u0627\u0644\u0643\u0634\u0641 \u0639\u0646 \u0642\u0627\u0628\u0644\u064a\u0629 \u0639\u0632\u0644\u0627\u062a \u0628\u0643\u062a\u0631\u064a\u0627 E.coli\u00a0 \u0639\u0644\u0649 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0628\u0627\u0639\u062a\u0645\u0627\u062f \u0637\u0631\u064a\u0642\u0629\u00a0 Microtiter Plate Method ( MTP)\u060c \u0648\u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0646 (%78) 39 \u0639\u0632\u0644\u0629 \u0644\u0647\u0627 \u0627\u0644\u0642\u0627\u0628\u0644\u064a\u0629 \u0639\u0644\u0649 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0648\u0628\u062f\u0631\u062c\u0627\u062a \u0645\u062a\u0641\u0627\u0648\u062a\u0629 \u060c \u0623\u0638\u0647\u0631\u062a (%8)4 \u0639\u0632\u0644\u0629 \u0644\u0647\u0627 \u0627\u0644\u0642\u0627\u0628\u0644\u064a\u0629 \u0639\u0644\u0649 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u0642\u0648\u064a \u0644\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0648 (%10)5 \u0639\u0632\u0644\u0629 \u0644\u0647\u0627 \u0627\u0644\u0642\u0627\u0628\u0644\u064a\u0629 \u0639\u0644\u0649 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u0645\u062a\u0648\u0633\u0637 \u0644\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0648\u0623\u0646 (%60)30 \u0639\u0632\u0644\u0629 \u0644\u0647\u0627 \u0627\u0644\u0642\u0627\u0628\u0644\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u0636\u0639\u064a\u0641 \u0644\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u060c \u0641\u064a\u0645\u0627 \u0623\u0638\u0647\u0631\u062a 11 \u0639\u0632\u0644\u0629 \u0628\u0646\u0633\u0628\u0629 (%22) \u063a\u064a\u0631 \u0642\u0627\u062f\u0631\u0629 \u0639\u0644\u0649 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a.<\/span><\/h4>\n

\u062f\u0631\u0633 \u062a\u0623\u062b\u064a\u0631 \u062a\u0631\u0627\u0643\u064a\u0632 \u0645\u062a\u062f\u0631\u062c\u0629 \u0645\u0646 \u062d\u0627\u0645\u0636 \u0627\u0644\u062e\u0644\u064a\u0643 (0.039 \u2013 5)% \u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 \u062a\u0623\u062b\u064a\u0631 \u0645\u0636\u0627\u062f Ceftazidime \u0628\u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 (4-512) \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644\u0644\u062a\u0631 \u0648\u0643\u0630\u0644\u0643 \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u062e\u0644\u0637\u064a \u0644\u0643\u0644\u0627\u0647\u0645\u0627 \u0639\u0644\u0649 \u062e\u0645\u0633\u0629 \u0639\u0632\u0644\u0627\u062a \u0628\u0643\u062a\u0631\u064a\u0647 \u0644 E.coli \u060c \u0627\u0646\u062a\u062e\u0628\u062a \u0648\u0641\u0642\u0627\u064b \u0644\u0642\u0627\u0628\u0644\u064a\u062a\u0647\u0627( \u0645\u0642\u0627\u0648\u0645\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0647 \u0648 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a ) \u0647\u064a (E9 , E10 , E18 , E25 , E41 ) . \u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062c\u0627\u0631\u0628 \u0623\u0646 \u062d\u0627\u0645\u0636 \u0627\u0644\u062e\u0644\u064a\u0643 \u0643\u0627\u0646 \u0644\u0647 \u062a\u0623\u062b\u064a\u0631 \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0648\u0627\u0637\u0626\u0629 \u0641\u064a\u0645\u0627 \u0628\u064a\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u062a\u0623\u062b\u064a\u0631 \u0645\u0636\u0627\u062f Ceftazidime \u0623\u0646 \u0644\u0647 \u062a\u0623\u062b\u064a\u0631 \u0645\u062b\u0628\u0637 \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0639\u0627\u0644\u064a\u0629 \u0648\u0627\u0644\u0645\u0646\u062e\u0641\u0636\u0629 \u060c \u0628\u064a\u0646\u0645\u0627 \u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u062e\u0644\u0637\u064a \u0644\u0647 \u062f\u0648\u0631 \u0645\u062b\u0628\u0637 \u0648\u0628\u062f\u0631\u062c\u0627\u062a \u0645\u062e\u062a\u0644\u0641\u0629.<\/span><\/h4>\n

\u0627\u062c\u0631\u0649 \u0627\u0644\u0643\u0634\u0641 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u0639\u0646 \u062a\u0648\u0627\u0641\u0631\u00a0 \u062c\u064a\u0646 \u0627\u0644\u0645\u0633\u0624\u0648\u0644 \u0639\u0646 \u062a\u0643\u0648\u064a\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a csgD (curli subunit gene D) \u0641\u064a20 \u0639\u0632\u0644\u0629 \u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0644 E.coli\u00a0 \u062a\u0645 \u0627\u062e\u062a\u064a\u0627\u0631\u0647\u0627 \u0648\u0641\u0642\u0627 \u0644\u0642\u062f\u0631\u062a\u0647\u0627 \u0639\u0644\u0649 \u0627\u0646\u062a\u0627\u062c \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062d\u064a\u0648\u064a \u0648\u0645\u0642\u0627\u0648\u0645\u062a\u0647\u0627 \u0644\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u060c \u0641\u0643\u0627\u0646\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0646 95% \u0645\u0646 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u062a\u062d\u0645\u0644 \u062c\u064a\u0646 csgD\u00a0 \u0648\u0628\u0648\u0632\u0646 \u062c\u0632\u064a\u0626\u064a 651 \u0632\u0648\u062c \u0642\u0627\u0639\u062f\u064a.<\/span><\/h4>\n

\u0628\u064a\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u062a\u062a\u0627\u0628\u0639\u064a Sequence \u0644\u0642\u0637\u0639 DNA \u0627\u0644\u0645\u0636\u062e\u0645\u0629 \u0641\u064a19 \u0639\u0632\u0644\u0629 \u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0644\u00a0\u00a0\u00a0 E.coli \u062a\u0639\u0648\u062f \u0644\u0644\u062c\u064a\u0646 csgD \u0648\u0630\u0644\u0643 \u0628\u0627\u0631\u0633\u0627\u0644 \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u0636\u062e\u064a\u0645 \u0628\u062a\u0642\u0627\u0646\u0629 PCR \u0628\u0627\u0644\u0627\u062a\u062c\u0627\u0647\u064a\u0646 \u0627\u0644\u0627\u0645\u0627\u0645\u064a\u0629 \u0648\u0627\u0644\u0631\u0627\u062c\u0639\u0629 .\u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u062a\u062a\u0627\u0628\u0639\u064a \u0648\u062c\u0648\u062f 7 \u0645\u062c\u0627\u0645\u064a\u0639 \u062c\u0645\u064a\u0639\u0647\u0627 \u0645\u0637\u0627\u0628\u0642\u0629 100% \u0645\u0639 \u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0639\u0627\u0644\u0645\u064a\u0629 \u0627\u0644\u0645\u0633\u062c\u0644\u0629 \u0641\u064a\u00a0\u00a0\u00a0 NCBI\u0645\u0627\u0639\u062f\u0627 \u0627\u0644\u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u062e\u0627\u0645\u0633\u0629 \u0627\u0644\u062a\u064a \u062a\u062d\u0648\u064a E20 \u062a\u0628\u064a\u0646 \u0648\u062c\u0648\u062f \u062a\u063a\u0627\u064a\u064a\u0631 \u0641\u064a \u0627\u0644\u0642\u0627\u0639\u062f\u0629 \u0627\u0644\u0646\u062a\u0631\u0648\u062c\u064a\u0646\u0629 Adenine \u0627\u0644\u0649 Guanine \u0641\u064a \u0627\u0644\u0645\u0648\u0642\u0639 46 \u0648\u0645\u0646 \u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0648\u062c\u062f \u0647\u0630\u0627 \u0627\u0644\u062a\u063a\u0627\u064a\u0631 \u0644\u0645 \u064a\u0624\u062b\u0631 \u0641\u064a \u0627\u0644\u062d\u0627\u0645\u0636 \u0644\u0630\u0627 \u0641\u0647\u0648 \u0637\u0641\u0631\u0629 \u0635\u0627\u0645\u062a\u0629 salient mutation.<\/span><\/h4>\n

<\/h4>\n

\u0648\u062e\u0644\u0635\u062a \u0627\u0644\u0637\u0627\u0644\u0628\u0629 \u0627\u0644\u0649 \u0627\u0644\u062a\u0648\u0635\u064a\u0627\u062a \u0627\u0644\u0627\u062a\u064a\u0629 :<\/span><\/h4>\n
    \n
  1. \n

    \u0627\u062c\u0631\u0627\u0621 \u062f\u0631\u0627\u0633\u0627\u062a \u062d\u0648\u0644 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u062d\u0648\u0627\u0645\u0636 \u0627\u0644\u0639\u0636\u0648\u064a\u0629 \u0627\u0644\u0627\u062e\u0631\u0649 \u0639\u0644\u0649 \u0639\u0648\u0627\u0645\u0644 \u0627\u0644\u0636\u0631\u0627\u0648\u0629 \u0641\u064a \u0627\u0644\u0628\u0643\u062a\u0631\u064a\u0627 \u0627\u0644\u0645\u0645\u0631\u0636\u0629.<\/span><\/h4>\n<\/li>\n
  2. \n

    \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a gene expression \u0644\u062c\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0644\u062d\u0648\u0627\u0645\u0636 \u0627\u0644\u0639\u0636\u0648\u064a\u0629 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062a\u0642\u0627\u0646\u0629 real time.<\/span><\/h4>\n<\/li>\n
  3. \n

    \u0636\u0631\u0648\u0631\u0629 \u0646\u0634\u0631 \u0627\u0644\u0648\u0639\u064a \u0627\u0644\u0635\u062d\u064a \u0648\u0639\u062f\u0645 \u0627\u0644\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0627\u0644\u0639\u0634\u0648\u0627\u0626\u064a \u0644\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0627\u062a\u064a\u0629 \u0648\u0628\u062f\u0648\u0646 \u0627\u0633\u062a\u0634\u0627\u0631\u0629 \u0637\u0628\u064a\u0629 \u0645\u0645\u0627 \u064a\u0624\u062f\u064a \u0627\u0644\u0649 \u0638\u0647\u0648\u0631 \u0633\u0644\u0627\u0644\u0627\u062a \u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0645\u0642\u0627\u0648\u0645\u0629.<\/span><\/h4>\n
    \n<\/li>\n<\/ol>\n

    Bacteriological and Molecular Study for The Effect of The mixture of Acetic Acid and Ceftazidime antibiotic on The growth and Biofilm Formation of Escherichia coli bacteria isolated From different clinical cases<\/span><\/h3>\n

    By\u00a0 Dua’a Rashid Humadi<\/span><\/h3>\n

    Supervised by Asst.prof.Dr.Sua’ad Khalil Ibrahim<\/span><\/h3>\n

    Abstract<\/span><\/h3>\n

    165 clinical samples, including 137 Urine samples from patients suffering from urinary tract infections, and 28 samples of Wound swabs from patients with different wounds, were screened to obtain pathological isolates of the genus E. coli.Samples were collected from different hospitals in the city of Baghdad (Alimameen Al-Kadhmin Medical City, Educational laboratories, Child Protection Education Hospital at Medical City Hospital, Al-Karkh General Hospital and Child Central Hospital) from 1\/8\/2019 to 10\/15\/2019<\/span><\/h3>\n

    The purified bacterial colonies were seeded with MacConkey agar, Eosin Methelen Blue (EMB), blood agar,Then he was diagnosed using biochemical tests and then he made the final diagnosis using the Vitek-2 Compact System. Diagnostic results showed that 50 isolates were due to E. coli bacteria, and it included (92%) 46 isolates from urine and (8%) 4 from wound swabs<\/span><\/h3>\n

    The sensitivity of the bacterial isolates was examined by the method of spreading tablets using 10 antibiotics. The results showed that the bacterial isolates were 43 isolates, including resistance to Trimethoprim and Piperacillin, at a rate of (86%),Of the 32 isolates resistant to anti-ceftazidime and ciprofloxacin (64%), 20 isolates were resistant to anti-cefoxitin (40%), 18 isolates were resistant to anti-Amikacin (36%), and 12 isolates were resistant to two anti-gentamicin and nitrofurantoin (24%).10 isolates were resistant to Chloramphenicol, at a rate (20%), and 2 isolates were resistant to anti-Imipenem, at a rate of (4%)<\/span><\/h3>\n

    The ability of E. coli isolates to form biofilm was revealed by using the Microtiter Plate Method (MTP).The results showed that (78%) 39 isolates have the ability to form a biofilm, and to varying degrees, 4 (8%) isolates showed the ability to form a strong biofilm.And (10%) 5 isolates have the ability to form the medium for the bio membrane and (60%) 30 isolates have the ability to weakly form the bio membrane, while 11 isolates showed (22%) are unable to form the bio membrane<\/span><\/h3>\n

    The effect of graduated concentrations of acetic acid (0.039 – 5)% compared to the effect of anti-ceftazidime with concentrations (4-512) g \/ ml as well as the mixture effect of both on five E. coli bacterial isolates were studied, Selected according to their ability (antibiotic resistance and biofilm formation) are (E9, E10, E18, E25, E41). The results of the experiments showed that acetic acid had an effect at low concentrations, while the results of the study showed that the anti-ceftazidime effect has an inhibitory effect at high and low concentrations, while the results of the mixture effect showed an inhibitory role and in different degrees<\/span><\/h3>\n

    Molecular detection of the availability of a gene responsible for the formation of the csgD biofilm (curli subunit gene D) was carried out in 20 isolates of E. coli selected according to their ability to produce the biofilm and their resistance to antibiotics. The results were that 95% of the bacterial isolates carry the csgD gene with a molecular weight 651 base pairs<\/span><\/h3>\n

    The results of the sequential analysis of the amplified DNA segments were shown in 19 E. coliisolates belonging to the csgD gene by sending the amplification results with PCR technology in the forward and backward directions. The results of the sequential analysis showed the existence of 7 groups, all of which were 100% identical with the global isolates registered in NCBI except for the fifth group that contains E20. It was found that there are changes in the nitrogen base Adenine to Guanine at site 46<\/span><\/h3>\n

    The present study aimed to investigate the effect of acetic acid and ceftazidime on the growth and formation of the biofilm of E. coli isolated from different clinical cases<\/span><\/h3>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

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