{"id":38748,"date":"2021-09-12T12:46:30","date_gmt":"2021-09-12T09:46:30","guid":{"rendered":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=38748"},"modified":"2021-09-14T12:20:36","modified_gmt":"2021-09-14T09:20:36","slug":"%d9%82%d8%b3%d9%85-%d8%b9%d9%84%d9%88%d9%85-%d8%a7%d9%84%d8%ad%d9%8a%d8%a7%d8%a9-%d9%8a%d9%86%d8%a7%d9%82%d8%b4-%d8%a3%d8%b7%d8%b1%d9%88%d8%ad%d8%a9-%d8%af%d9%83%d8%aa%d9%88%d8%b1%d8%a7%d9%87-%d8%aa-4","status":"publish","type":"post","link":"https:\/\/ihcoedu.uobaghdad.edu.iq\/?p=38748","title":{"rendered":"\u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u064a\u0646\u0627\u0642\u0634 \u0623\u0637\u0631\u0648\u062d\u0629 \u062f\u0643\u062a\u0648\u0631\u0627\u0647 \u062a\u062f\u0631\u0633 \u062c\u064a\u0646\u0627\u062a \u0639\u0627\u0645\u0644 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0645\u062d\u0648\u0651\u0644 – \u0628\u064a\u062a\u0627 TGF-\u03b2 \u0648\u0645\u0633\u062a\u0642\u0628\u0644\u0647 \u0627\u0644\u062b\u0627\u0644\u062b TGF-\u03b2RIII \u0648\u0645\u0633\u062a\u0636\u062f \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062f\u0645 \u0627\u0644\u0628\u064a\u0636 \u0627\u0644\u0628\u0634\u0631\u064a\u0629 \u0646\u0648\u0639 -\u062c – HLA-G \u0641\u064a \u0627\u0644\u0646\u0633\u0627\u0621 \u0627\u0644\u0639\u0631\u0627\u0642\u064a\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u0628\u0627\u062a \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a"},"content":{"rendered":"

\"\"<\/strong><\/span><\/h4>\n

\u0646\u0627\u0642\u0634 \u0642\u0633\u0645 \u0639\u0644\u0648\u0645 \u0627\u0644\u062d\u064a\u0627\u0629 \u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u062a\u0631\u0628\u064a\u0629 \u0644\u0644\u0639\u0644\u0648\u0645 \u0627\u0644\u0635\u0631\u0641\u0629 (\u0627\u0628\u0646 \u0627\u0644\u0647\u064a\u062b\u0645) \u0623\u0637\u0631\u0648\u062d\u0629 \u0627\u0644\u062f\u0643\u062a\u0648\u0631\u0627\u0647 \u0627\u0644\u0645\u0648\u0633\u0648\u0645\u0629 (\u062f\u0631\u0627\u0633\u0629 \u0648\u0631\u0627\u062b\u064a\u0629 \u0645\u0646\u0627\u0639\u064a\u0629 \u0644\u062c\u064a\u0646\u0627\u062a \u0639\u0627\u0645\u0644 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0645\u062d\u0648\u0651\u0644 – \u0628\u064a\u062a\u0627 <\/strong>TGF-\u03b2<\/em><\/strong> \u0648\u0645\u0633\u062a\u0642\u0628\u0644\u0647 \u0627\u0644\u062b\u0627\u0644\u062b <\/strong>TGF-\u03b2RIII<\/em><\/strong> \u0648\u0645\u0633\u062a\u0636\u062f \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062f\u0645 \u0627\u0644\u0628\u064a\u0636 \u0627\u0644\u0628\u0634\u0631\u064a\u0629 \u0646\u0648\u0639 -\u062c – <\/strong>HLA-G<\/em><\/strong> \u0641\u064a \u0627\u0644\u0646\u0633\u0627\u0621 \u0627\u0644\u0639\u0631\u0627\u0642\u064a\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u0628\u0627\u062a \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a) \u0644\u0644\u0637\u0627\u0644\u0628 (\u0639\u0627\u062f\u0644 \u0639\u0645\u0631\u0627\u0646 \u0633\u0644\u0645\u0627\u0646) \u0627\u0644\u062a\u064a \u0627\u0646\u062c\u0632\u0647\u0627 \u062a\u062d\u062a \u0627\u0634\u0631\u0627\u0641 \u062a\u062f\u0631\u064a\u0633\u064a\u062a\u0627 \u0627\u0644\u0642\u0633\u0645 (\u0623.\u0645.\u062f. \u062d\u0627\u0632\u0645\u0629 \u0645\u0648\u0633\u0649 \u062e\u0644\u064a\u0644 ) \u0648\u00a0 (\u0623.\u0645.\u062f. \u0648\u0641\u0627\u0621 \u0635\u0628\u0631\u064a \u0645\u0627\u0647\u0648\u062f) \u0648\u0646\u0648\u0642\u0634\u062a \u0645\u0646 \u0642\u0628\u0644 \u0623\u0639\u0636\u0627\u0621 \u0644\u062c\u0646\u0629 \u0627\u0644\u0645\u0646\u0627\u0642\u0634\u0629 \u0627\u0644\u0645\u0628\u064a\u0646\u0629 \u0623\u0633\u0645\u0627\u0626\u0647\u0645 \u0641\u064a\u0645\u0627 \u064a\u0623\u062a\u064a :<\/strong><\/span><\/h4>\n
    \n
  1. \n

    \u0627.\u062f. \u0627\u062d\u0633\u0627\u0646 \u0639\u0631\u0641\u0627\u0646 \u062d\u0633\u064a\u0646 (\u0631\u0626\u064a\u0633\u0627\u064b)<\/strong><\/span><\/h4>\n<\/li>\n
  2. \n

    \u0627.\u062f. \u062d\u0645\u064a\u062f \u0645\u062c\u064a\u062f \u062c\u0627\u0633\u0645 (\u0639\u0636\u0648\u0627\u064b)<\/strong><\/span><\/h4>\n<\/li>\n
  3. \n

    \u0627.\u062f. \u0627\u0628\u0631\u0627\u0647\u064a\u0645 \u0647\u0627\u062f\u064a \u0645\u062d\u0645\u062f (\u0639\u0636\u0648\u0627\u064b)<\/strong><\/span><\/h4>\n<\/li>\n
  4. \n

    \u0627.\u0645.\u062f. \u0632\u0647\u0631\u0627\u0621 \u062d\u0633\u064a\u0646 \u0645\u062d\u0645\u062f \u0642\u062f\u0631\u064a (\u0639\u0636\u0648\u0627\u064b) <\/strong><\/span><\/h4>\n<\/li>\n
  5. \n

    \u0627.\u0645.\u062f. \u0647\u0646\u0627\u062f\u064a \u0633\u0627\u0644\u0645 \u0639\u0628\u062f \u0627\u0644\u0635\u0627\u062d\u0628 (\u0639\u0636\u0648\u0627\u064b)<\/strong><\/span><\/h4>\n<\/li>\n
  6. \n

    \u0623.\u0645.\u062f. \u062d\u0627\u0632\u0645\u0629 \u0645\u0648\u0633\u0649 \u062e\u0644\u064a\u0644 (\u0639\u0636\u0648\u0627 \u0648 \u0645\u0634\u0631\u0641\u0627)<\/strong><\/span><\/h4>\n<\/li>\n
  7. \n

    \u0623.\u0645.\u062f. \u0648\u0641\u0627\u0621 \u0635\u0628\u0631\u064a \u0645\u0627\u0647\u0648\u062f (\u0639\u0636\u0648\u0627 \u0648\u0645\u0634\u0631\u0641\u0627)<\/strong><\/span><\/h4>\n<\/li>\n<\/ol>\n

    \"\"<\/h4>\n

    \u0647\u062f\u0641\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0625\u0644\u0649 \u062a\u0642\u064a\u064a\u0645 \u062f\u0648\u0631 \u0627\u0644\u062c\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u062b\u0628\u0637\u0629 \u0648\u0627\u0644\u062a\u0646\u0638\u064a\u0645\u064a\u0629 \u0644\u0643\u0644 \u0645\u0646 (\u0639\u0627\u0645\u0644 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0645\u062d\u0648\u0651\u0644 \u2013 \u0628\u064a\u062a\u0627 TGF-\u03b2\u00a0 \u0648\u0645\u0633\u062a\u0642\u0628\u0644\u0647 \u0627\u0644\u062b\u0627\u0644\u062b \u00a0TGF-\u03b2RIII \u0648\u0645\u0633\u062a\u0636\u062f \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062f\u0645 \u0627\u0644\u0628\u064a\u0636 \u0627\u0644\u0628\u0634\u0631\u064a\u0629 \u0646\u0648\u0639 -\u062c- \u00a0HLA-G) \u0641\u064a \u0627\u0644\u062a\u0633\u0628\u0628 \u0641\u064a \u0627\u0644\u0625\u0635\u0627\u0628\u0629 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a \u0644\u062f\u0649 \u0628\u0639\u0636 \u0627\u0644\u0646\u0633\u0627\u0621 \u0627\u0644\u0639\u0631\u0627\u0642\u064a\u0627\u062a \u060c \u0648\u0644\u062a\u062d\u0642\u064a\u0642 \u0630\u0644\u0643 \u062a\u0645 \u0627\u0639\u062a\u0645\u0627\u062f \u0627\u0644\u0639\u062f\u064a\u062f \u0645\u0646 \u0627\u0644\u062e\u0637\u0648\u0627\u062a \u0627\u0644\u0627\u062a\u064a\u0629 :<\/span><\/h4>\n
      \n
    1. \n

      \u062a\u062d\u062f\u064a\u062f \u0627\u0644\u0642\u064a\u0627\u0633\u0627\u062a \u0627\u0648 \u0627\u0644\u062e\u0635\u0627\u0626\u0635 \u0627\u0644\u0628\u0634\u0631\u064a\u0629 \u0648\u0627\u0644\u0645\u0631\u0636\u064a\u0629 \u0644\u062c\u0645\u064a\u0639 \u0627\u0644\u0645\u0631\u0636\u0649 \u0648\u062a\u0642\u064a\u064a\u0645 \u0627\u0631\u062a\u0628\u0627\u0637 \u0628\u0639\u0636 \u0627\u0644\u062e\u0635\u0627\u0626\u0635 \u0628\u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a Gene expression \u0648\u0628\u062a\u0639\u062f\u062f \u0627\u0644\u0623\u0634\u0643\u0627\u0644 \u0627\u0644\u062c\u064a\u0646\u064a Gene polymorphism \u0644\u0644\u062c\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u062b\u0628\u0637\u0629 \u0648\u0627\u0644\u062a\u0646\u0638\u064a\u0645\u064a\u0629 \u0627\u0644\u062a\u0627\u0644\u064a\u0629 HLA-G , TGF-\u03b21 , TGF-\u03b23 \u0648 TGF-\u03b2RIII.<\/span><\/h4>\n<\/li>\n
    2. \n

      \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u0646\u0645\u064a\u0637 \u0627\u0644\u062c\u064a\u0646\u064a Genotyping \u0644\u062a\u0639\u062f\u062f \u0627\u0644\u0623\u0634\u0643\u0627\u0644 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0644\u062c\u064a\u0646\u0627\u062a HLA-G \u060c TGF-\u03b21 \u0648 TGF-\u03b2RIII \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0627\u0646\u0629 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a Conventional PCR \u0648\u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062a\u062a\u0627\u0628\u0639\u064a \u00a0\u0644\u0644\u062d\u0645\u0636 \u0627\u0644\u0646\u0648\u0648\u064a DNA sequencing.<\/span><\/h4>\n<\/li>\n
    3. \n

      \u062a\u062d\u062f\u064a\u062f \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0648\u062a\u0642\u064a\u064a\u0645\u0647 \u0644\u0644\u062c\u064a\u0646\u0627\u062a HLA-G\u060c\u00a0 TGF-\u03b23 \u0648 TGF-\u03b2RIII \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0646\u064a\u0629 \u0627\u0644\u0648\u0642\u062a \u0627\u0644\u062d\u0642\u064a\u0642\u064a \u0644\u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0643\u0645\u064a (RT-qPCR).<\/span><\/h4>\n<\/li>\n<\/ol>\n

      \u00a0<\/strong><\/span><\/h4>\n

      \u00a0<\/strong><\/span><\/h4>\n

      \u062c\u0645\u064a\u0639 \u0639\u064a\u0646\u0627\u062a \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u062a\u0645 \u062c\u0645\u0639\u0647\u0627 \u0645\u0646 \u0627\u0644\u0646\u0633\u0627\u0621 \u0627\u0644\u0639\u0631\u0628\u064a\u0627\u062a \u0627\u0644\u0639\u0631\u0627\u0642\u064a\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u0628\u0627\u062a \u062d\u062f\u064a\u062b\u0627\u064b \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a \u0628\u0645\u062a\u0648\u0633\u0637 \u0639\u0645\u0631 (51.18 \u00b1 10.64) \u0628\u0627\u0644\u0625\u0636\u0627\u0641\u0629 \u0625\u0644\u0649 40 \u0639\u064a\u0646\u0629 \u062f\u0645 \u0645\u0646 \u0627\u0644\u0646\u0633\u0627\u0621 \u0627\u0644\u0623\u0635\u062d\u0627\u0621 \u0643\u0645\u062a\u0637\u0648\u0639\u0627\u062a (\u0633\u064a\u0637\u0631\u0629) . \u062a\u0645\u062a \u0645\u0642\u0627\u0628\u0644\u0629 \u0627\u0644\u0646\u0633\u0627\u0621 \u0627\u0644\u0623\u0635\u062d\u0627\u0621 \u0648\u0627\u0644\u0645\u0631\u064a\u0636\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u0628\u0627\u062a\u00a0 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a \u0648\u0627\u0633\u062a\u062c\u0648\u0627\u0628\u0647\u0646 \u0648\u0641\u0642 \u0627\u0633\u062a\u0645\u0627\u0631\u0629 \u062e\u0627\u0635\u0629 \u0648\u0627\u0644\u0645\u062f\u0631\u062c\u0629 \u0641\u064a \u0627\u0644\u0645\u0644\u0627\u062d\u0642 \u060c \u0644\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0645\u0639\u0644\u0648\u0645\u0627\u062a \u0627\u0644\u0642\u064a\u0627\u0633\u0627\u062a \u0627\u0644\u0628\u0634\u0631\u064a\u0629 , \u0628\u064a\u0646\u0645\u0627 \u062a\u0645 \u0627\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0627\u0644\u062e\u0635\u0627\u0626\u0635 \u0627\u0644\u0633\u0631\u064a\u0631\u064a\u0629 \u0648\u0627\u0644\u0646\u0633\u064a\u062c\u064a\u0629 \u0644\u0623\u0648\u0631\u0627\u0645 \u0627\u0644\u0645\u0631\u0636\u0649 \u0645\u0646 \u0633\u062c\u0644\u0627\u062a\u0647\u0645 \u0627\u0644\u0637\u0628\u064a\u0629 \u060c \u0628\u0639\u062f \u0627\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0627\u0644\u0645\u0648\u0627\u0641\u0642\u0629 \u0627\u0644\u0623\u062e\u0644\u0627\u0642\u064a\u0629.<\/span><\/h4>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u062a\u0636\u0645\u0646\u062a \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u062c\u0632\u0623\u064a\u0646 \u0645\u0646 \u062f\u0631\u0627\u0633\u0627\u062a \u0627\u0644\u0648\u0631\u0627\u062b\u0629 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 , \u0634\u0645\u0644 \u0627\u0644\u0627\u0648\u0644 \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u0646\u0645\u064a\u0637 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u062c\u064a\u0646\u0627\u062a \u0645\u0633\u062a\u0636\u062f \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062f\u0645 \u0627\u0644\u0628\u064a\u0636 \u0627\u0644\u0628\u0634\u0631\u064a\u0629 \u0646\u0648\u0639-\u062c- HLA-G \u00a0, \u0639\u0627\u0645\u0644 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0645\u062d\u0648\u0651\u0644 \u2013 \u0628\u064a\u062a\u06271 TGF-\u03b21 \u0648\u0645\u0633\u062a\u0642\u0628\u0644\u0647 \u0627\u0644\u062b\u0627\u0644\u062b \u00a0TGF-\u03b2RIII\u0648\u0627\u0644\u062a\u064a \u0627\u062c\u0631\u064a\u062a \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0627\u0646\u0629 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a (PCR) \u0648\u062a\u0645 \u0627\u0644\u062a\u062d\u0642\u0642 \u0645\u0646 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0628\u0623\u0633\u062a\u0639\u0645\u0627\u0644 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062a\u062a\u0627\u0628\u0639\u064a Sequencing\u00a0 . \u0648\u0627\u0644\u062b\u0627\u0646\u064a \u0634\u0645\u0644 \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0648\u062a\u0642\u064a\u064a\u0645\u0647 \u0628\u0623\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0627\u0646\u0629 \u0627\u0644\u0648\u0642\u062a \u0627\u0644\u062d\u0642\u064a\u0642\u064a\u00a0 \u0644\u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0643\u0645\u064a (RT-qPCR) \u00a0\u0644\u062c\u064a\u0646\u0627\u062a HLA-G , TGF-\u03b23 \u0648 TGF-\u03b2RIII. \u0644\u062a\u062d\u0644\u064a\u0644 \u062c\u0632\u064a\u0626\u0627\u062a\u0647\u0627 \u0627\u0644\u0645\u062b\u0628\u0637\u0629 \u0648\u0627\u0644\u062a\u0646\u0638\u064a\u0645\u064a\u0629 \u0648\u062a\u0642\u064a\u064a\u0645 \u062f\u0648\u0631\u0647\u0627 \u0641\u064a \u0627\u0644\u062a\u0633\u0628\u0628 \u0641\u064a \u0627\u0644\u0625\u0635\u0627\u0628\u0629 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a \u0644\u062f\u0649 \u0628\u0639\u0636 \u0627\u0644\u0625\u0646\u0627\u062b \u0627\u0644\u0639\u0631\u0628\u064a\u0627\u062a \u0627\u0644\u0639\u0631\u0627\u0642\u064a\u0627\u062a.<\/span><\/h4>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u062a\u0636\u0645\u0646\u062a \u062f\u0631\u0627\u0633\u0629 \u062a\u0639\u062f\u062f \u0627\u0644\u0623\u0634\u0643\u0627\u0644 \u0627\u0644\u062c\u064a\u0646\u064a \u0627\u0644\u0645\u0638\u0647\u0631\u064a \u0644\u0643\u0644 \u0645\u0646 \u0627\u0644\u062c\u064a\u0646\u0627\u062a \u0627\u0644\u062a\u0627\u0644\u064a\u0629 :\u00a0 \u0645\u0633\u062a\u0636\u062f \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062f\u0645 \u0627\u0644\u0628\u064a\u0636 \u0627\u0644\u0628\u0634\u0631\u064a\u0629 \u0646\u0648\u0639 \u2013\u062c\u00a0 HLA-G \u00a0\u0648\u0627\u0645\u0643\u0627\u0646\u064a\u0629 \u062a\u062d\u062f\u064a\u062f \u0627\u0644\u0639\u0644\u0627\u0642\u0629 \u0627\u0644\u0645\u0631\u0627\u0641\u0642\u0629 \u0628\u064a\u0646 \u062a\u0643\u0631\u0627\u0631 \u062a\u0639\u062f\u062f \u0627\u0644\u0623\u0634\u0643\u0627\u0644 (14 \u0632\u0648\u062c \u0642\u0627\u0639\u062f\u0629) \u00a0.14-bp insertion\/deletion \u00a0\u0641\u064a \u0627\u0644\u0645\u0646\u0637\u0642\u0629 \u0627\u0644\u0645\u0634\u0641\u0631\u0629 8 \u0644\u0644\u0645\u0646\u0637\u0642\u0629 \u063a\u064a\u0631 \u0627\u0644\u0645\u062a\u0631\u062c\u0645\u0629 3` \u0645\u0639 \u062e\u0637\u0631 \u0627\u0644\u0623\u0635\u0627\u0628\u0629 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a \u060c \u0639\u0627\u0645\u0644 \u0627\u0644\u0646\u0645\u0648\u0627\u0644\u0645\u062d\u0648\u0651\u0644- \u0628\u064a\u062a\u0627 1 ( (TGF-\u03b21 \u0648\u0627\u0644\u0645\u0633\u062a\u0642\u0628\u0644 \u0627\u0644\u062b\u0627\u0644\u062b \u0644\u0639\u0627\u0645\u0644 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0645\u062d\u0648\u0651\u0644-\u0628\u064a\u062a\u0627\u00a0 (TGF-\u03b2RIII) \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0627\u0646\u0629 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a (PCR) \u00a0. \u0648\u062a\u0645 \u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062a\u062a\u0627\u0628\u0639\u064a\u064a \u00a0Sequencing \u0644\u0644\u062d\u0627\u0645\u0636 \u0627\u0644\u0646\u0648\u0648\u064a DNA \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0627\u0646\u0629 \u0633\u0627\u0646\u062c\u0631 \u00a0Sanger \u0644\u0645\u0639\u0631\u0641\u0629 \u0627\u0644\u062a\u0646\u0648\u0639 \u0627\u0644\u062c\u064a\u0646\u064a \u0641\u064a \u0645\u0646\u0637\u0642\u0629 \u0627\u0644\u0645\u062d\u0641\u0632 Promoter region \u00a0\u0644\u0643\u0644 \u0645\u0646 \u062c\u064a\u0646\u064a\u00a0 TGF-\u03b21 \u00a0\u0648\u0645\u0633\u062a\u0642\u0628\u0644\u0647 \u0627\u0644\u062b\u0627\u0644\u062b TGF-\u03b2RIII.<\/span><\/h4>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0643\u0645\u0627 \u062a\u0645 \u00a0\u062a\u062d\u062f\u064a\u062f \u0645\u0633\u062a\u0648\u0649 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0643\u0644 \u0645\u0646 \u00a0\u0627\u0644\u062c\u064a\u0646\u0627\u062a \u0627\u0644\u062a\u0627\u0644\u064a\u0629: \u00a0HLA-G , TGF-\u03b23 \u0648 TGF-\u03b2RIII \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0643\u0645\u064a \u0641\u064a \u0627\u0644\u0648\u0642\u062a \u0627\u0644\u062d\u0642\u064a\u0642\u064a (RT-qPCR) \u0648\u062a\u062d\u062f\u064a\u062f \u0627\u0644\u062e\u0635\u0627\u0626\u0635 \u0644\u0644\u0642\u064a\u0627\u0633\u0627\u062a \u0627\u0644\u0628\u0634\u0631\u064a\u0629 \u0648\u0627\u0644\u0645\u0631\u0636\u064a\u0629 \u0644\u062c\u0645\u064a\u0639 \u0627\u0644\u0645\u0631\u0636\u0649 \u0648\u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u0627\u0631\u062a\u0628\u0627\u0637 \u0628\u064a\u0646 \u0647\u0630\u0647 \u0627\u0644\u0645\u0633\u062a\u0648\u064a\u0627\u062a \u0645\u0646 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0648\u062a\u0639\u062f\u062f \u0627\u0644\u0623\u0634\u0643\u0627\u0644 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0644\u062c\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u062b\u0628\u0637\u0629 \u0648\u0627\u0644\u062a\u0646\u0638\u064a\u0645\u064a\u0629 HLA-G , TGF-\u03b21 , TGF- \u03b23 \u0648 TGF-\u03b2RIII.<\/span><\/h4>\n

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      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0627\u0633\u062a\u062e\u062f\u0645\u062a \u062a\u0642\u0627\u0646\u0629 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a \u0644\u062a\u0643\u062b\u064a\u0631 \u0627\u0644\u062c\u064a\u0646 \u0648\u0627\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0647\u0630\u0627 \u0627\u0644\u062a\u0642\u0627\u0646\u0629 \u0627\u062b\u0646\u064a\u0646 \u0645\u0646 \u0627\u0644\u062d\u0632\u0645 ( \u0627\u062f\u062e\u0627\u0644 +14\u00a0 \u0627\u0648 \u062d\u0630\u0641 -14\u00a0 ) \u0644\u0644\u0642\u0637\u0639\u0629 – 14\u0632\u0648\u062c \u0642\u0627\u0639\u062f\u0629 \u0644\u0644\u062c\u064a\u0646 HLA-G \u0648\u0627\u0644\u062a\u064a \u0643\u0627\u0646\u062a 210 \u0632\u0648\u062c \u0642\u0627\u0639\u062f\u0629 \u0627\u0648224 \u0632\u0648\u062c \u0642\u0627\u0639\u062f\u0629 \u00a0\u0628\u0627\u0644\u062a\u0639\u0627\u0642\u0628 \u0648\u0627\u0644\u062a\u064a \u0645\u064a\u0632\u062a \u0628\u0648\u0627\u0633\u0637\u0629 \u0627\u0644\u062a\u0631\u062d\u064a\u0644 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0639\u0644\u0649 \u0647\u0644\u0627\u0645 \u0627\u0644\u0623\u0643\u0631\u0648\u0632 2%.<\/span><\/h4>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0 \u0643\u0634\u0641\u062a \u0646\u062a\u0627\u0626\u062c \u062a\u0639\u062f\u062f \u0627\u0644\u0623\u0634\u0643\u0627\u0644 \u0627\u0644\u0648\u0631\u0627\u062b\u064a \u0644\u062c\u064a\u0646 HLA-G \u0639\u0646 \u062b\u0644\u0627\u062b\u0629 \u0623\u0646\u0645\u0627\u0637 \u0648\u0631\u0627\u062b\u064a\u0629: \u0645\u062a\u0645\u0627\u062b\u0644 \u0627\u0644\u062c\u064a\u0646\u0627\u062a +14bp\/+14bp (\u0627\u062f\u062e\u0627\u0644 \/ \u0627\u062f\u062e\u0627\u0644) \u060c \u0645\u062a\u0645\u0627\u062b\u0644 \u0627\u0644\u062c\u064a\u0646\u0627\u062a -14bp\/-14bp ( \u062d\u0630\u0641 \/ \u062d\u0630\u0641) \u0648\u0645\u062a\u063a\u0627\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u0627\u062a \u00a0+14bp\/-14bp( \u0627\u062f\u062e\u0627\u0644 \/ \u062d\u0630\u0641 ) \u060c \u0643\u0634\u0641\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0623\u0646 \u0627\u0644\u062a\u0643\u0631\u0627\u0631\u0627\u062a \u0627\u0644\u0645\u0642\u062f\u0631\u0629 \u0644\u0644\u0637\u0631\u0627\u0632 \u0627\u0644\u0648\u0631\u0627\u062b\u064a \u0645\u062a\u0645\u0627\u062b\u0644 \u0627\u0644\u062c\u064a\u0646\u0627\u062a) +14bp\/+14bp \u0627\u062f\u062e\u0627\u0644\/ \u0627\u062f\u062e\u0627\u0644 ) \u0643\u0627\u0646\u062a \u0623\u0639\u0644\u0649 \u0628\u064a\u0646 \u0627\u0644\u0645\u0631\u0636\u0649 (26.7%) \u0645\u0642\u0627\u0628\u0644 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u00a0(12.5%) \u00a0(OR=2.55, 95%CI=0.86-7.54 , P=0.088) \u00a0. \u0648\u0638\u0647\u0631 \u0647\u0630\u0627 \u0627\u0644\u0646\u0645\u0637 \u0643\u0637\u0631\u0627\u0632 \u0648\u0631\u0627\u062b\u064a \u0645\u0633\u0628\u0628 \u0648\u0645\u0631\u062a\u0628\u0637 \u0628\u0627\u0644\u0645\u0631\u0636 Etiological fraction(EF) \u0628\u0644\u063a\u062a \u0642\u064a\u0645\u062a\u0647 0.16 , \u0643\u0645\u0627 \u0643\u0627\u0646\u062a \u0642\u064a\u0645\u0629 \u0627\u0644\u0646\u0633\u0628\u0629 \u0627\u0644\u062d\u0631\u062c\u0629 OR \u062a\u0633\u0627\u0648\u064a 2.55 \u0648\u0645\u062f\u0629 \u0627\u0644\u062b\u0642\u0629 CI \u0643\u0627\u0646\u062a \u0642\u064a\u0645\u062a\u0647\u0627 \u0628\u064a\u0646 0.86-7.54 \u00a0\u00a0\u00a0\u0648\u0628\u0627\u0644\u0645\u062b\u0644 \u060c \u0643\u0627\u0646 \u062a\u0631\u062f\u062f \u0623\u0644\u064a\u0644 \u0627\u0644\u0625\u062f\u062e\u0627\u0644 (ins ) HLA-G 14-bp \u0623\u0639\u0644\u0649 \u0641\u064a \u0627\u0644\u0645\u0631\u0636\u0649 (51.7\u066a) \u0645\u0642\u0627\u0628\u0644 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 (40\u066a) ( OR=1.60, 95%CI=0.91-2.83 , P=0.105 ) \u060c \u0648\u0638\u0647\u0631 \u0627\u0644\u064a\u0644 \u0627\u0644\u0623\u062f\u062e\u0627\u0644 ins \u0643\u0627\u0644\u064a\u0644 \u0645\u0633\u0628\u0628 Etiological fraction(EF) \u0628\u0644\u063a\u062a \u0642\u064a\u0645\u062a\u0647 0.19 \u0648\u0645\u0631\u062a\u0628\u0637 \u0645\u0639 \u0627\u0644\u0645\u0631\u0636, \u0627\u0630 \u0643\u0627\u0646\u062a \u0642\u064a\u0645\u0629 \u0627\u0644\u0646\u0633\u0628\u0629 \u0627\u0644\u062d\u0631\u062c\u0629 OR \u062a\u0633\u0627\u0648\u064a 1.60 \u0648\u0645\u062f\u0629 \u0627\u0644\u062b\u0642\u0629 CI \u0643\u0627\u0646\u062a \u0642\u064a\u0645\u062a\u0647\u0627 \u0628\u064a\u0646 0.91-2.83 \u00a0\u00a0,\u00a0 \u0645\u0639 \u0639\u062f\u0645 \u0648\u062c\u0648\u062f \u0641\u0631\u0648\u0642 \u0630\u0627\u062a \u062f\u0644\u0627\u0644\u0629 \u0625\u062d\u0635\u0627\u0626\u064a\u0629 \u0641\u064a \u0645\u0633\u062a\u0648\u064a\u0627\u062a \u0627\u0644\u0646\u0645\u0637 \u0627\u0644\u062c\u064a\u0646\u064a \u0648\u0627\u0644\u0623\u0644\u064a\u0644\u064a \u0628\u064a\u0646 \u0627\u0644\u0645\u0631\u0636\u0649 \u0648\u0645\u062c\u0645\u0648\u0639\u0627\u062a \u0627\u0644\u0633\u064a\u0637\u0631\u0629 \u0639\u0646\u062f \u0645\u0633\u062a\u0648\u0649 \u0627\u062d\u062a\u0645\u0627\u0644\u064a\u0629 p\u22640.05 \u0648\u0639\u0646\u062f \u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0627\u062e\u062a\u0628\u0627\u0631 \u0641\u064a\u0634\u0631\u060c \u0648\u062a\u0634\u064a\u0631 \u0647\u0630\u0647 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u0625\u0644\u0649 \u0648\u062c\u0648\u062f \u0627\u0631\u062a\u0628\u0627\u0637 \u0628\u064a\u0646 \u0627\u0644\u0642\u0627\u0628\u0644\u064a\u0629 \u0644\u0644\u0625\u0635\u0627\u0628\u0629 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a \u0648\u0627\u0644\u0646\u0645\u0637 \u0627\u0644\u0648\u0631\u0627\u062b\u064a\u00a0 \u00a0\u0627\u062f\u062e\u0627\u0644 \/ \u0627\u062f\u062e\u0627\u0644 \u0648\u0627\u0644\u064a\u0644\u0647 \u0644\u0644\u062c\u064a\u0646 HLA-G 14-bp \u060c \u064a\u0645\u0643\u0646 \u0627\u0644\u0646\u0638\u0631 \u0641\u064a\u0647 \u0643\u0639\u0627\u0645\u0644 \u062e\u0637\u0631 \u0648\u0631\u0627\u062b\u064a \u0645\u062d\u062a\u0645\u0644 \u0641\u064a \u0628\u062f\u0621 \u0648\u062a\u0637\u0648\u0631 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a.<\/span><\/h4>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0643\u0634\u0641\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062a\u062a\u0627\u0628\u0639\u064a Sequencing \u0648\u062a\u0639\u062f\u062f \u0627\u0644\u0623\u0634\u0643\u0627\u0644 \u0627\u0644\u0648\u0631\u0627\u062b\u064a \u0644\u0644\u062c\u064a\u0646 \u00a0TGF-\u03b21 509T\/C\u00a0\u00a0 (rs1800469) \u0639\u0646 \u062b\u0644\u0627\u062b\u0629 \u0637\u0631\u0632 \u0648\u0631\u0627\u062b\u064a\u0629 (TT \u060c \u00a0TC \u0648 CC). \u0648\u0648\u062c\u062f \u0623\u0646 \u0627\u0644\u0646\u0645\u0637 \u0627\u0644\u0648\u0631\u0627\u062b\u064a \u0645\u062a\u063a\u0627\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u0627\u062a (TC) \u0633\u062c\u0644 \u062a\u0631\u062f\u062f\u064b\u0627 \u0639\u0627\u0644\u064a\u064b\u0627 \u0641\u064a \u0627\u0644\u0645\u0631\u0636\u0649 32\/60 (53.3%) \u0628\u0627\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 19\/40(47.5%) \u060c \u0648\u0643\u0627\u0646 OR=1.26, 95%CI=0.57-2.79 , P=0.568)) , \u0648\u0643\u0630\u0644\u0643 \u0643\u0627\u0646 \u062a\u0631\u062f\u062f \u0627\u0644\u0646\u0645\u0637 \u0627\u0644\u0648\u0631\u0627\u062b\u064a \u0645\u062a\u0645\u0627\u062b\u0644 \u0627\u0644\u062c\u064a\u0646\u0627\u062a (TT)\u00a0 \u0647\u0648 \u0627\u0644\u0623\u062e\u0631 \u0623\u0639\u0644\u0649 \u0642\u0644\u064a\u0644\u0627\u064b \u0641\u064a \u0627\u0644\u0645\u0631\u0636\u0649 11\/60 (18.3%) \u0645\u0646 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 7\/40(17.5%) OR=1.06, 95%CI=0.38-2.98 , P=0.915))\u00a0 . \u0648\u0638\u0647\u0631 \u0647\u0630\u0627\u0646 \u0627\u0644\u0646\u0645\u0637\u0627\u0646 \u0627\u0644\u0648\u0631\u0627\u062b\u064a\u0627\u0646 TT \u0648 TC \u0643\u0646\u0645\u0637\u064a\u0646 \u0648\u0631\u0627\u062b\u064a\u064a\u0646 \u0645\u0633\u0628\u0628\u064a\u0646 \u0648\u0645\u0631\u062a\u0628\u0637\u064a\u0646 \u0645\u0639 \u062e\u0637\u0631 \u0627\u0644\u0623\u0635\u0627\u0628\u0629 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a Etiological fraction(EF) \u0628\u0644\u063a\u062a \u0642\u064a\u0645\u062a\u0647 0.01 \u0648 0.11 , \u0639\u0644\u0649 \u0627\u0644\u062a\u0648\u0627\u0644\u064a. \u0648\u0628\u0627\u0644\u0645\u062b\u0644 \u060c \u0643\u0627\u0646 \u062a\u0648\u0627\u062a\u0631 \u0627\u0644\u0623\u0644\u064a\u0644 T \u0623\u0639\u0644\u0649 \u0641\u064a \u0627\u0644\u0645\u0631\u0636\u0649 (45\u066a) \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0627\u0644\u0636\u0648\u0627\u0628\u0637 (41.3\u066a) (OR = 1.17 , 95% CI=0.66-2.06 , P = 0.6) \u060c \u0648\u0638\u0647\u0631 \u0643\u0627\u0644\u064a\u0644 \u0645\u0633\u0628\u0628 Etiological fraction(EF) \u0628\u0644\u063a\u062a \u0642\u064a\u0645\u062a\u0647 0.06 \u0648\u0645\u0631\u062a\u0628\u0637 \u0645\u0639 \u0627\u0644\u0645\u0631\u0636 \u0641\u064a \u0639\u064a\u0646\u0629 \u0627\u0644\u0645\u0635\u0627\u0628\u064a\u0646 , \u0628\u064a\u0646\u0645\u0627 \u0638\u0647\u0631 \u0627\u0644\u0623\u0644\u064a\u0644 C \u0643\u0623\u0644\u064a\u0644 \u0648\u0642\u0627\u0626\u064a \u0645\u0646 \u0627\u0644\u0645\u0631\u0636 Preventive fraction (PF) \u0648\u0628\u0644\u063a\u062a \u0642\u064a\u0645\u062a\u0647 \u00a00.08\u060c \u0645\u0639 \u0639\u062f\u0645 \u0648\u062c\u0648\u062f \u0641\u0631\u0648\u0642 \u0630\u0627\u062a \u062f\u0644\u0627\u0644\u0629 \u0625\u062d\u0635\u0627\u0626\u064a\u0629 \u0628\u064a\u0646 \u0627\u0644\u0645\u0631\u0636\u0649 \u0648\u0627\u0644\u0636\u0648\u0627\u0628\u0637 \u0639\u0646\u062f \u0645\u0633\u062a\u0648\u0649 \u0627\u062d\u062a\u0645\u0627\u0644\u064a\u0629 ( p\u22640.05) , \u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u0648\u062c\u0648\u062f \u0627\u0631\u062a\u0628\u0627\u0637 \u0628\u064a\u0646 \u0627\u0644\u0642\u0627\u0628\u0644\u064a\u0629 \u0644\u0644\u0625\u0635\u0627\u0628\u0629 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a \u0648\u0627\u0644\u0646\u0645\u0637\u064a\u0646 \u0627\u0644\u062c\u064a\u0646\u064a\u064a\u0646 TC \u0648 TGF-\u03b21 (rs 1800469) TT \u0643\u0639\u0627\u0645\u0644 \u062e\u0637\u0631 \u0648\u0631\u0627\u062b\u064a \u0645\u062d\u062a\u0645\u0644 \u0641\u064a \u062a\u0637\u0648\u0631 \u0627\u0644\u0645\u0631\u0636 \u0625\u0630\u0627 \u062a\u0645 \u062a\u062d\u0631\u064a\u0636\u0647 \u0628\u0648\u0627\u0633\u0637\u0629 \u0627\u0644\u0639\u0648\u0627\u0645\u0644 \u0627\u0644\u0628\u064a\u0626\u064a\u0629 \u0627\u0644\u062e\u0627\u0631\u062c\u064a\u0629 \u0641\u064a \u0627\u0644\u0623\u0641\u0631\u0627\u062f \u0627\u0644\u0645\u062c\u0647\u0632\u064a\u0646 \u0648\u0631\u0627\u062b\u064a\u0627 .<\/span><\/h4>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0623\u0638\u0647\u0631\u062a \u0646\u062a\u064a\u062c\u0629 \u0627\u0644\u062a\u0636\u062e\u064a\u0645 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0644\u062c\u064a\u0646 TGF-\u03b2RIII \u0646\u0637\u0627\u0642\u064b\u0627 \u0648\u0627\u062d\u062f\u064b\u0627 \u0644\u0643\u0644 \u0639\u064a\u0646\u0629 \u062c\u0632\u064a\u0626\u064a\u0629 (457 \u0646\u0642\u0637\u0629 \u0623\u0633\u0627\u0633) \u0648\u0646\u062a\u0627\u0626\u062c \u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062d\u0645\u0636 \u0627\u0644\u0646\u0648\u0648\u064a \u0648\u062a\u0639\u062f\u062f \u0627\u0644\u0623\u0634\u0643\u0627\u0644 \u0627\u0644\u0648\u0631\u0627\u062b\u064a \u0623\u0638\u0647\u0631\u062a \u062b\u0644\u0627\u062b\u0629 \u0637\u0631\u0632 \u0648\u0631\u0627\u062b\u064a\u0629 (GG \u060c \u00a0GC \u0648 CC). \u0623\u0638\u0647\u0631\u062a \u0647\u0630\u0647 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u0627\u0631\u062a\u0628\u0627\u0637\u064b\u0627 \u0628\u064a\u0646 \u0642\u0627\u0628\u0644\u064a\u0629 \u0627\u0644\u0625\u0635\u0627\u0628\u0629 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u0648\u0627\u0644\u0637\u0631\u0627\u0632 \u0627\u0644\u0645\u0638\u0647\u0631\u064a TGF-\u03b2RIII 457-bp \u00a0CC \u0648 \u0627\u0644\u064a\u0644\u0647 C \u0643\u0639\u0627\u0645\u0644 \u062e\u0637\u0631 \u0648\u0631\u0627\u062b\u064a \u0645\u062d\u062a\u0645\u0644 \u0641\u064a \u062a\u0637\u0648\u0631 \u0627\u0644\u0645\u0631\u0636.<\/span><\/h4>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0638\u0647\u0631 \u0627\u0644\u0623\u0644\u064a\u0644 C \u0643\u0627\u0644\u064a\u0644 \u0645\u0633\u0628\u0628 Etiological fraction(EF) \u0628\u0644\u063a\u062a \u0642\u064a\u0645\u062a\u0647 0.04 \u0648\u0645\u0631\u062a\u0628\u0637 \u0645\u0639 \u0627\u0644\u0645\u0631\u0636 \u0641\u064a \u0639\u064a\u0646\u0629 \u0627\u0644\u0645\u0635\u0627\u0628\u064a\u0646 , \u0628\u064a\u0646\u0645\u0627 \u0638\u0647\u0631 \u0627\u0644\u0623\u0644\u064a\u0644 G \u0643\u0623\u0644\u064a\u0644 \u0648\u0642\u0627\u0626\u064a \u0645\u0646 \u0627\u0644\u0645\u0631\u0636 Preventive fraction (PF) . \u0627\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0623\u062d\u0635\u0627\u0626\u064a \u0644\u0644\u0646\u0645\u0637 \u0627\u0644\u0648\u0631\u0627\u062b\u064a CC \u0646\u0633\u0628\u0629 \u0627\u0639\u0644\u0649 \u0639\u0646\u062f \u0627\u0644\u0645\u0631\u0636\u0649 3.3% \u00a0\u0628\u0627\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 \u0627\u0644\u0633\u064a\u0637\u0631\u0629 0.0% \u0648\u0638\u0647\u0631 \u0647\u0630\u0627 \u0627\u0644\u0646\u0645\u0637 \u0643\u0646\u0645\u0637 \u0648\u0631\u0627\u062b\u064a \u0645\u0631\u062a\u0628\u0637 \u0645\u0639 \u062e\u0637\u0631 \u0627\u0644\u0623\u0635\u0627\u0628\u0629 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a.<\/span><\/h4>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062c\u0632\u064a\u0626\u064a\u0629 (\u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a) \u0632\u064a\u0627\u062f\u0629 \u0645\u0639\u0646\u0648\u064a\u0629 \u0639\u0627\u0644\u064a\u0629 \u0641\u064a \u0645\u0633\u062a\u0648\u0649 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u062f\u0649 \u0627\u0644\u0645\u0631\u0636\u0649 0.54 \u00b1 0.62 \u0648 4.57 \u00b1 5.50 \u0644\u062c\u064a\u0646\u0627\u062a HLA-G \u0648 TGF-\u03b23 \u0639\u0644\u0649 \u0627\u0644\u062a\u0648\u0627\u0644\u064a \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u062a\u062d\u0643\u0645 (0.28 \u00b1 0.23) \u0648 2.64 \u00b1 2.49) \u0644\u062c\u064a\u0646\u0627\u062a HLA-G \u0648 TGF\u03b23 \u00a0, \u0639\u0644\u0649 \u0627\u0644\u062a\u0648\u0627\u0644\u064a. \u0628\u064a\u0646\u0645\u0627 \u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u0623\u0646 \u0637\u064a\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 TGF-\u03b2RIII \u0642\u062f \u0627\u0646\u062e\u0641\u0636\u062a \u0641\u064a \u0645\u0631\u0636\u0649 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a (1.457 \u00b1 2.10) \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0627\u0644\u0636\u0648\u0627\u0628\u0637 (2.10 \u00b1 2.63) \u0645\u0639 \u0639\u062f\u0645 \u0648\u062c\u0648\u062f \u0641\u0631\u0642 \u0645\u0639\u0646\u0648\u064a \u0639\u0646\u062f P\u22640.05.<\/span><\/h4>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0 \u0648\u0641\u064a \u0627\u0644\u062e\u062a\u0627\u0645 \u060c \u062a\u0634\u064a\u0631 \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062d\u0627\u0644\u064a\u0629 \u0625\u0644\u0649 \u0623\u0646 \u062a\u0639\u062f\u062f \u0627\u0644\u0623\u0634\u0643\u0627\u0644 \u0627\u0644\u0623\u0643\u062b\u0631\u062a\u0643\u0631\u0627\u0631\u0627\u064b \u0647\u0648 \u0627\u0644\u0646\u0645\u0637 \u0627\u0644\u062c\u064a\u0646\u064a \u0627\u0644\u0645\u062a\u0645\u0627\u062b\u0644 \u0644\u0644\u0625\u062f\u062e\u0627\u0644 insertion\/insertion \u0648\u0623\u0644\u064a\u0644\u0647 insertion \u00a0\u0644\u0644\u062c\u064a\u0646 \u0640 HLA-G 14-bp \u0641\u064a \u0627\u0644\u0646\u0633\u0627\u0621 \u0627\u0644\u0639\u0631\u0627\u0642\u064a\u0627\u062a \u0627\u0644\u0645\u0635\u0627\u0628\u0627\u062a \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a \u060c \u0648\u064a\u0645\u0643\u0646 \u0627\u0639\u062a\u0628\u0627\u0631\u0647 \u0639\u0627\u0645\u0644 \u062e\u0637\u0631 \u0648\u0631\u0627\u062b\u064a \u0645\u062d\u062a\u0645\u0644 \u0641\u064a \u0628\u062f\u0621 \u0648 \u062a\u0637\u0648\u0631 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a . \u0641\u064a \u062d\u064a\u0646 \u0623\u0646 \u0627\u0644\u0646\u0645\u0637\u064a\u0646 \u0627\u0644\u062c\u064a\u0646\u064a\u064a\u0646 TT \u0648 TC \u0644\u062a\u0639\u062f\u062f \u0627\u0644\u0623\u0634\u0643\u0627\u0644 \u0644\u0644\u062c\u064a\u0646 TGF-\u03b21 \u00a0\u0648\u0627\u0644\u0646\u0645\u0637 \u0627\u0644\u062c\u064a\u0646\u064aCC \u00a0\u0644\u0644\u062c\u064a\u0646 TGF\u03b2RIII \u00a0\u0648\u0623\u0644\u064a\u0644\u0647 C \u0642\u062f \u064a\u0639\u0632\u0632\u0627\u0646 \u0642\u0627\u0628\u0644\u064a\u0629 \u0627\u0644\u0625\u0635\u0627\u0628\u0629 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a , \u0643\u0645\u0627 \u0633\u062c\u0644\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0632\u064a\u0627\u062f\u0629 \u0645\u0639\u0646\u0648\u064a\u0629 \u0639\u0627\u0644\u064a\u0629 \u0641\u064a \u0645\u0633\u062a\u0648\u0649 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0644\u0643\u0644 \u0645\u0646 \u062c\u064a\u0646 HLA-G \u0648\u062c\u064a\u0646 TGF-\u03b23 \u0644\u062f\u0649 \u0645\u0631\u0636\u0649 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a, \u0648\u0623\u062e\u064a\u0631\u064b\u0627 \u0648\u062c\u062f\u062a \u0623\u0646 \u0637\u064a\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u062c\u064a\u0646 TGF-\u03b2RIII \u0642\u062f \u0627\u0646\u062e\u0641\u0636\u062a \u0641\u064a \u0645\u0631\u0636\u0649 \u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u062b\u062f\u064a \u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0628\u0627\u0644\u0636\u0648\u0627\u0628\u0637.<\/span><\/h4>\n
      \n

      Immunogenetic Study on TGF-\u03b2, TGF-\u03b2RIII and HLA-G Genes among\u00a0<\/span><\/h3>\n

      \u00a0Iraqi Women wih Breast Cancer<\/span><\/h3>\n

      By Adel Omran Salman Hasan<\/span><\/h3>\n

      Supervised by Asst.prof.Hazima Mossa \u00a0and Asst.prof.Waffa’a Sabri Mahmood<\/span><\/h3>\n

      Aims of the Study\u00a0<\/span><\/h3>\n

      \u00a0\u00a0\u00a0\u00a0 This investigation aimed to evaluate the role of inhibitory and regulatory genes HLA-G, TGF-\u03b21, TGF-\u03b23 and TGF-\u03b2RIII in the pathogenesis of breast cancer in some Iraqi females, so to achieve this goals \u00a0,many objectives were adopted<\/span><\/h3>\n

      Determine the Anthropometric and pathological characteristics for all patients and asses the correlation of some properties with the gene expression and gene polymorphism for the inhibitory and regulatory genes HLA-G, TGF-\u03b21, TGF-\u03b23 and TGF-\u03b2RIII<\/span><\/h3>\n

      Geno typing the HLA-G, TGF-\u03b21and TGF-\u03b2RIII genes polymorphism by using the conventional PCR technique and DNA sequencing<\/span><\/h3>\n

      Determine the gene expression of HLA-G, TGF-\u03b23 and TGF-\u03b2RIII genes by using Real time quantitative PCR technique (RT-qPCR)<\/span><\/h3>\n

      Abstract<\/span><\/h3>\n

      The study was aimed to investigate the role of inhibitory and regulatory genes in the pathogenesis of breast carcinoma. This study was included 60 Peripheral blood samples which withdrawn from early diagnosed breast cancer patients which attended to Oncology Teaching Hospital of the Medical City in Baghdad at the period from May 2019 until February 2020. All collected samples from Iraqi women suffer from breast cancer with average age mean 51.18 \u00b1 10.64 in addition to 40 blood samples from apparently healthy women as volunteers. The healthy women and patients with cancer were interviewed and underwent to a questionnaire according to a special form which listed in appendices to get the anthropometric information, however, the clinical and histological properties about the patients tumors were obtained from their medical report, in addition ethical approval.<\/span><\/h3>\n

      This study was implied two-parts of immunogenetic studies, the first is the genotyping of human leukocyte antigen \u2013G (HLA-G), transforming growth factor-beta1 (TGF-\u03b21) and transforming growth factor beta receptor III (TGF-\u03b2RIII) genes were performed by conventional polymerase chain reaction (PCR). The results were verified by sequencing technique. The second is the gene expression, which was evaluated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) technique for HLA-G, TGF-\u03b23 and TGF-\u03b2RIII genes, for analyzing their inhibitory, regulatory genes and assessing their role in the pathogenesis of breast cancer in some Iraqi females.<\/span><\/h3>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0 In regard to HLA-G, it has been investigated the possible etiological role of 14-bp insertion\/deletion polymorphism in exon 8 of 3` untranslated region (3`UTR) with the risk and predisposition of breast cancer, while TGF-\u03b21 and TGF-\u03b2RIII were investigated by DNA sequencing using automated Sanger technique to determine the genetic variation in the promoter region of both TGF-\u03b21 and TGF-\u03b2RIIIgenes. In addition to determine the level of gene expression for HLA-G, TGF-\u03b23and TGF-\u03b2RIII genes by using RT-qPCR. The investigation of the association of some anthropometric and pathological characteristics with gene polymorphism and gene expression for all of the inhibitory and regulatory genes (i.e. HLA-G, TGF-\u03b21, TGF- \u03b23 and TGF-\u03b2RIII).<\/span><\/h3>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 The results of this study showed high significant differences between the most demographic and clinico pathological characteristics of breast cancer women. The highest incidence of breast cancer noted among women in their fifth and sixth decades of life, and the most breast cancer women in current study were Estrogen receptor (ER),Progesterone receptor (PR) positive and human epidermal growth factor receptor-2 (HER-2\/neu) negative. The majority of breast cancer in Iraqi women was invasive breast carcinoma (100%) in which invasive ductal carcinoma constitutes 91.7%, and the most aggressive molecular sub type was luminal like A carcinoma. Most histological tumor grades were 76.7% for moderately differentiated (GII) and Stage II were 43.3%. The results of tumor size showed that 46 \/60 (76.7%) of patients were with more than 2 cm tumor size and 37\/60(61.7%) of patients had lymph node metastasis.<\/span><\/h3>\n

      PCR amplification was used for detection of insertion or deletion of the 14bp sequence in HLA-G generated PCR products of length 210bp or 224bp, respectively, and distinguished by 2% agarose gel electrophoresis.<\/span><\/h3>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Genetic polymorphism results of HLA-G gene were observed with three genotypes: homozygous for the +14bp\/+14bp (ins\/ins), homozygous -14bp\/-14bp (del\/del), and heterozygous +14bp\/-14bp (ins \/del).The results revealed that estimated frequencies of the homozygous +14bp\/+14bp (ins\/ins) genotype was higher among patients 16\/60 (26.7%) versus controls 5\/40(12.5%) (OR = 2.55, 95% CI= 0.86-7.54, P=0.088), this genotype revealed as Etiological fraction (EF) and correlated with risk by having this disease. Similarly, the HLA-G 14-bp insertion allele (ins) frequency was higher in patients (51.7%) versus controls (40.0%) (OR=1.60, 95% CI=0.91-2.83, P=0.105), the (ins) allele revealed as Etiological fraction (EF) and correlated with the disease in patients sample, With no significant difference found at allelic and geno type levels between the case and control groups at P\u22640.05, and according to Fishers exact probability.\u00a0 These present results suggested an association between breast cancer susceptibility and HLA-G 14-bp ins\/ins and its allele (ins), it may be consider as a potential genetic risk factor in the initiation and progression of the breast cancer.<\/span><\/h3>\n

      \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 The results of DNA sequencing and genetic polymorphism of TGF-\u03b21 gene 509 T\/C (rs1800469) showed three genotypes TT, TC and CC. The heterozygous genotype (TC) was recorded high frequency in patients 32\/60 (53.3%) as compared with controls 19\/40 (47.5%), and the OR was 1.26 (95% CI = (0.57-2.79, P=0.568). The homozygous genotype (TT) frequency was slightly higher in patients 11\/60 (18.3%) than controls 7\/40 (17.5%) (OR= 1.06, 95% CI = 0.38-2.98, P= 0.915). The TT and TC genotypes revealed as etiological fraction (EF) with risk by having this disease. Similarly the frequency of the T allele was higher in patients (45%) versus controls (41.3%) (OR= 1.17, 95% CI = 0.66-2.06, P= 0.6), and revealed as Etiological fraction (EF) and correlated with the disease. Whereas the C allele revealed as Preventive fraction (PF). With no significant differences recorded between patients and controls at (P\u22640.05) and according to Fishers exact probability. Present results showed an association between breast cancer susceptibility and TGF-\u03b21(rs 1800469). This study demonstrates that the T-509 C SNP (TT and TC)geno types are significant clinical risk factor in the progression of the disease.<\/span><\/h3>\n

      The result of TGF-\u03b2RIII gene amplification showed a single band for each sample with 457 bp molecular and the results of DNA sequencing and polymorphism showed three genotypes GG, GC and CC, also the results showed an association between breast cancer susceptibility and TGF-\u03b2RIII 457-bp (CC and C) as a potential genetic risk factor in the initiation progression of the disease. The C allele revealed as (EF) and correlated with the disease in patient’s sample, whereas the G allele revealed as Preventive fraction (PF). The homozygous genotype (CC) frequency was higher in patients (3.3%) versus controls (0.0%) and this genotype revealed as (EF) correlated with breast cancer.<\/span><\/h3>\n

      Gene expression results showed high significant increasing in level of gene expression in patients 0.54 \u00b1 0.62 and 4.57 \u00b1 5.50 for HLA-G and TGF-\u03b23 genes, respectively, as compared to control group 0.28 \u00b1 0.23 and 2.64 \u00b1 2.49. While, the present results showed that expression fold of TGF-\u03b2RIIIgene was decreased in breast cancer patients (1.457 \u00b1 2.10) as compared to controls (2.10 \u00b1 2.63) with no significant difference was found. A significant difference was noted between expression fold of HLA-G gene and genotype frequency for TGF-\u03b2RIII in breast cancer patients P=0.013. Also, a significant association was noted between expression fold of TGF-\u03b2RIII gene with genotype frequency for TGF\u03b21 in breast cancer patients P=0.001. A higher level of expression of the TGF-\u03b23 gene correlated with patients at categories of 40-49 years of age (P=0.005).<\/span><\/h3>\n

      In conclusion, the results of the current study demonstrated, that the most frequencies polymorphism is the homozygous insertion genotype of HLA-G 14-bp and its allele in Iraqi Arabian breast cancer women, and may consider a potential genetic risk factor in the initiation and progression of the breast cancer. While, TT and TC genotype for TGF-\u03b21polymorphism and TGF-\u03b2RIII CC genotype and its allele C may enhance the susceptibility of breast cancer. Also, the results was recorded that high significant increasing of HLA-G and TGF-\u03b23 gene expression level in breast cancer patients, and finally found that expression fold of TGF-\u03b2RIII gene was decreased in breast cancer patients as compared to controls.<\/span><\/h3>\n

       <\/p>\n

       <\/p>\n

       <\/p>\n

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